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• W1539926464 abstract "Successful penetration and colonization of plant tissues by most fungal pathogens requires differentiation of specialised cell types or infection structures, e.g. germ tubes, appressoria, penetration hyphae, infection hyphae and haustoria. Each cell type is adapted to a particular role in the infection process, e.g. adhesion, contact-sensing, penetration and nutrient uptake [22,32]. Molecular genetic techniques, such as differential or subtractive hybridization and mutational analysis, are being used to identify genes involved in the morphogenesis and function of these infection structures. For example, many genes that are specifically expressed or up-regulated during the formation of appressoria by Colletotrichum, Magnaporthe and rust fungi have now been cloned [7,8,23,24,27,29,55,69,76]. Some of these genes have been sequenced and disrupted to determine their role in the infection process [24,69]. These approaches have so far been restricted to infection structures that can be obtained in vitro, such as appressoria. Identification of genes expressed by infection structures formed following host penetration is more difficult due to contamination with host mRNAs, although recent advances in the isolation of such structures from infected tissue may alleviate this problem [19,30,50]." @default.
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• W1539926464 date "1996-01-01" @default.
• W1539926464 modified "2023-09-27" @default.
• W1539926464 title "Use of Monoclonal Antibodies to Study Differentiation of Colletotrichum Infection Structures" @default.
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• W1539926464 doi "https://doi.org/10.1007/978-94-009-0189-6_5" @default.
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