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- W1539989113 abstract "Publisher Summary Chondroitin/dermatan sulfate (CS/DS) is a glycosaminoglycan (GAG) found in extracellular matrix (ECM) and cell surface and participates in various ECM and cell–ECM interactions. It is made from the alternate addition of glucuronic acid (GlcA) and N‐acetylgalactosamine (GalNAc). However, during biosynthesis, CS/DS is sulfated on C‐4 and/or C‐6 of GalNAc and on C‐2 of GlcA and a part of GlcA is epimerized on C‐5 to obtain iduronic acid (IdoA), all steps depend on the tissue or cell status, which makes the molecule more complex. Because of the complexity of CS/DS, its analysis is useful to characterize the molecules present in a sample and identify alterations with respect to their amounts and fine structural features in various pathological conditions. This chapter presents the various techniques developed to analyze and characterize CS/DS. The analysis of CS/DS is used for either quantitative purposes or its characterization. Very simple assays of high efficiency are developed for analytical determinations, with or without prior separation or degradation of the other GAGs, such as chromogenic, electrophoretic, and solid phase assays. In addition, sophisticated techniques are proposed in the chapter for CS/DS structural characterization in highly purified samples, such as high performance liquid chromatography, capillary electrophoresis (CE), and hyphenated CE." @default.
- W1539989113 created "2016-06-24" @default.
- W1539989113 creator A5035139391 @default.
- W1539989113 creator A5087331521 @default.
- W1539989113 creator A5090015352 @default.
- W1539989113 date "2006-01-01" @default.
- W1539989113 modified "2023-10-15" @default.
- W1539989113 title "Advances in the Analysis of Chondroitin/Dermatan Sulfate" @default.
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