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- W1540070911 abstract "Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected simultaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality properties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat." @default.
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- W1540070911 date "2009-12-01" @default.
- W1540070911 modified "2023-10-17" @default.
- W1540070911 title "Molecular identification on <I>Waxy</I> genes in wheat using multiple-PCR" @default.
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- W1540070911 doi "https://doi.org/10.3724/sp.j.1005.2009.00844" @default.
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