Matches in SemOpenAlex for { <https://semopenalex.org/work/W1540607130> ?p ?o ?g. }
- W1540607130 abstract "Brucella microti was first isolated from common vole (Microtus arvalis) in the Czech Republic in Central Europe in 2007. As B. microti is the only Brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. The present paper is the first to report the isolation of B. microti from a wild boar (Sus scrofa), which is also the first isolation of this bacterial species in Hungary.The B. microti isolate was cultured, after enrichment in Brucella-selective broth, from the submandibular lymph node of a female wild boar that was taken by hunters in Hungary near the Austrian border in September 2014. Histological and immunohistological examinations of the lymph node sections with B. abortus-, B. suis- and B. canis-specific sera gave negative results. The isolate did not require CO2 for growth, was oxidase, catalase, and urease positive, H2S negative, grew well in the presence of 20 μg/ml basic fuchsin and thionin, and had brownish pigmentation after three days of incubation. It gave strong positive agglutination with anti-A and anti-M but had a negative reaction with anti-R monospecific sera. The API 20 NE test identified it as Ochrobactrum anthropi with 99.9% identity, and it showed B. microti-specific banding pattern in the Bruce- and Suis-ladder multiplex PCR systems. Whole genome re-sequencing identified 30 SNPs in orthologous loci when compared to the B. microti reference genome available in GenBank, and the MLVA analysis yielded a unique profile.Given that the female wild boar did not develop any clinical disease, we hypothesize that this host species only harboured the bacterium, serving as a possible reservoir capable of maintaining and spreading this pathogen. The infectious source could have been either a rodent, a carcass that had been eaten or infection occurred via the boar rooting in soil. The low number of discovered SNPs suggests an unexpectedly high level of genetic homogeneity in this Brucella species." @default.
- W1540607130 created "2016-06-24" @default.
- W1540607130 creator A5014486771 @default.
- W1540607130 creator A5025604246 @default.
- W1540607130 creator A5048580851 @default.
- W1540607130 creator A5052291920 @default.
- W1540607130 creator A5066895711 @default.
- W1540607130 creator A5073944050 @default.
- W1540607130 creator A5074156203 @default.
- W1540607130 creator A5074401641 @default.
- W1540607130 creator A5081213334 @default.
- W1540607130 creator A5089779047 @default.
- W1540607130 date "2015-07-11" @default.
- W1540607130 modified "2023-10-18" @default.
- W1540607130 title "First isolation and characterization of Brucella microti from wild boar" @default.
- W1540607130 cites W1488262302 @default.
- W1540607130 cites W1978441703 @default.
- W1540607130 cites W1987298030 @default.
- W1540607130 cites W1989171740 @default.
- W1540607130 cites W1999823269 @default.
- W1540607130 cites W2020974424 @default.
- W1540607130 cites W2047389850 @default.
- W1540607130 cites W2054658353 @default.
- W1540607130 cites W2067867661 @default.
- W1540607130 cites W2092625885 @default.
- W1540607130 cites W2102780033 @default.
- W1540607130 cites W2121948244 @default.
- W1540607130 cites W2126638331 @default.
- W1540607130 cites W2127157018 @default.
- W1540607130 cites W2129767391 @default.
- W1540607130 cites W2132984763 @default.
- W1540607130 cites W2133981823 @default.
- W1540607130 cites W2136882867 @default.
- W1540607130 cites W2152895327 @default.
- W1540607130 cites W2164922079 @default.
- W1540607130 doi "https://doi.org/10.1186/s12917-015-0456-z" @default.
- W1540607130 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4499207" @default.
- W1540607130 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26163135" @default.
- W1540607130 hasPublicationYear "2015" @default.
- W1540607130 type Work @default.
- W1540607130 sameAs 1540607130 @default.
- W1540607130 citedByCount "35" @default.
- W1540607130 countsByYear W15406071302016 @default.
- W1540607130 countsByYear W15406071302017 @default.
- W1540607130 countsByYear W15406071302018 @default.
- W1540607130 countsByYear W15406071302019 @default.
- W1540607130 countsByYear W15406071302020 @default.
- W1540607130 countsByYear W15406071302021 @default.
- W1540607130 countsByYear W15406071302022 @default.
- W1540607130 countsByYear W15406071302023 @default.
- W1540607130 crossrefType "journal-article" @default.
- W1540607130 hasAuthorship W1540607130A5014486771 @default.
- W1540607130 hasAuthorship W1540607130A5025604246 @default.
- W1540607130 hasAuthorship W1540607130A5048580851 @default.
- W1540607130 hasAuthorship W1540607130A5052291920 @default.
- W1540607130 hasAuthorship W1540607130A5066895711 @default.
- W1540607130 hasAuthorship W1540607130A5073944050 @default.
- W1540607130 hasAuthorship W1540607130A5074156203 @default.
- W1540607130 hasAuthorship W1540607130A5074401641 @default.
- W1540607130 hasAuthorship W1540607130A5081213334 @default.
- W1540607130 hasAuthorship W1540607130A5089779047 @default.
- W1540607130 hasBestOaLocation W15406071301 @default.
- W1540607130 hasConcept C159047783 @default.
- W1540607130 hasConcept C18903297 @default.
- W1540607130 hasConcept C2776222705 @default.
- W1540607130 hasConcept C2777692091 @default.
- W1540607130 hasConcept C2780767784 @default.
- W1540607130 hasConcept C2781429187 @default.
- W1540607130 hasConcept C2909519439 @default.
- W1540607130 hasConcept C86803240 @default.
- W1540607130 hasConcept C89423630 @default.
- W1540607130 hasConceptScore W1540607130C159047783 @default.
- W1540607130 hasConceptScore W1540607130C18903297 @default.
- W1540607130 hasConceptScore W1540607130C2776222705 @default.
- W1540607130 hasConceptScore W1540607130C2777692091 @default.
- W1540607130 hasConceptScore W1540607130C2780767784 @default.
- W1540607130 hasConceptScore W1540607130C2781429187 @default.
- W1540607130 hasConceptScore W1540607130C2909519439 @default.
- W1540607130 hasConceptScore W1540607130C86803240 @default.
- W1540607130 hasConceptScore W1540607130C89423630 @default.
- W1540607130 hasIssue "1" @default.
- W1540607130 hasLocation W15406071301 @default.
- W1540607130 hasLocation W15406071302 @default.
- W1540607130 hasLocation W15406071303 @default.
- W1540607130 hasLocation W15406071304 @default.
- W1540607130 hasLocation W15406071305 @default.
- W1540607130 hasOpenAccess W1540607130 @default.
- W1540607130 hasPrimaryLocation W15406071301 @default.
- W1540607130 hasRelatedWork W174856138 @default.
- W1540607130 hasRelatedWork W1991529736 @default.
- W1540607130 hasRelatedWork W2069109401 @default.
- W1540607130 hasRelatedWork W2413484059 @default.
- W1540607130 hasRelatedWork W2415239303 @default.
- W1540607130 hasRelatedWork W3006390267 @default.
- W1540607130 hasRelatedWork W3096653437 @default.
- W1540607130 hasRelatedWork W3145785099 @default.
- W1540607130 hasRelatedWork W3148116695 @default.
- W1540607130 hasRelatedWork W3151226851 @default.
- W1540607130 hasVolume "11" @default.
- W1540607130 isParatext "false" @default.