Matches in SemOpenAlex for { <https://semopenalex.org/work/W1540618931> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1540618931 abstract "A2 Introduction: Temozolomide (TMZ) is an effective agent in the treatment of metastatic melanoma whose in-vivo anti-tumor activity correlates directly with O6-methylguanine-DNA methyltransferase (MGMT) activity. In this study, we examined whether MGMT RNA expression could serve as a surrogate marker for MGMT activity. The use of a PCR-based assay could facilitate clinical trials involving TMZ, particularly when only small tumor samples are available.Methods: The in-vitro sensitivities of 40 melanoma cell lines to TMZ was determined using a high-throughput, cytotoxic assay (Promega CellTiter Blue assay). Resistance to TMZ was defined as ≥55% sensitivity index across 0-3mM. MGMT RNA expression and activity were measured using quantitative PCR and HLPC, respectively. High MGMT RNA level was defined as ≥0.15 relative to MGMT expression in HT29 cell line. High MGMT activity was defined as ≥150 fmol/mg. Correlation analyses were performed where Pearson9s correlation coefficients were determined for MGMT expression and activity against TMZ sensitivities. Qualitative analyses were also performed using the criteria outlined above. Mismatch repair status of the cell lines were determined by testing for microsatellite instability using PCR and ABI Genescan 3100 analyzer (Applied Biosystem) with five mononucleotide markers (BAT-25, BAT-26, NR-21, NR-22, and NR-25).Results: There was a strong correlation between MGMT RNA expression and MGMT activity (correlation coefficient 0.84, p-value" @default.
- W1540618931 created "2016-06-24" @default.
- W1540618931 creator A5004785534 @default.
- W1540618931 creator A5008586187 @default.
- W1540618931 creator A5015827608 @default.
- W1540618931 creator A5016994371 @default.
- W1540618931 creator A5017076359 @default.
- W1540618931 creator A5031189107 @default.
- W1540618931 creator A5040746764 @default.
- W1540618931 creator A5064513935 @default.
- W1540618931 date "2006-10-01" @default.
- W1540618931 modified "2023-09-25" @default.
- W1540618931 title "Predicting temozolomide resistance with MGMT RNA expression in metastatic melanoma" @default.
- W1540618931 hasPublicationYear "2006" @default.
- W1540618931 type Work @default.
- W1540618931 sameAs 1540618931 @default.
- W1540618931 citedByCount "0" @default.
- W1540618931 crossrefType "journal-article" @default.
- W1540618931 hasAuthorship W1540618931A5004785534 @default.
- W1540618931 hasAuthorship W1540618931A5008586187 @default.
- W1540618931 hasAuthorship W1540618931A5015827608 @default.
- W1540618931 hasAuthorship W1540618931A5016994371 @default.
- W1540618931 hasAuthorship W1540618931A5017076359 @default.
- W1540618931 hasAuthorship W1540618931A5031189107 @default.
- W1540618931 hasAuthorship W1540618931A5040746764 @default.
- W1540618931 hasAuthorship W1540618931A5064513935 @default.
- W1540618931 hasConcept C104317684 @default.
- W1540618931 hasConcept C180754005 @default.
- W1540618931 hasConcept C207001950 @default.
- W1540618931 hasConcept C2776194525 @default.
- W1540618931 hasConcept C2777389519 @default.
- W1540618931 hasConcept C2777658100 @default.
- W1540618931 hasConcept C2779767149 @default.
- W1540618931 hasConcept C33288867 @default.
- W1540618931 hasConcept C502942594 @default.
- W1540618931 hasConcept C54355233 @default.
- W1540618931 hasConcept C552990157 @default.
- W1540618931 hasConcept C61320498 @default.
- W1540618931 hasConcept C71924100 @default.
- W1540618931 hasConcept C86803240 @default.
- W1540618931 hasConcept C91965660 @default.
- W1540618931 hasConceptScore W1540618931C104317684 @default.
- W1540618931 hasConceptScore W1540618931C180754005 @default.
- W1540618931 hasConceptScore W1540618931C207001950 @default.
- W1540618931 hasConceptScore W1540618931C2776194525 @default.
- W1540618931 hasConceptScore W1540618931C2777389519 @default.
- W1540618931 hasConceptScore W1540618931C2777658100 @default.
- W1540618931 hasConceptScore W1540618931C2779767149 @default.
- W1540618931 hasConceptScore W1540618931C33288867 @default.
- W1540618931 hasConceptScore W1540618931C502942594 @default.
- W1540618931 hasConceptScore W1540618931C54355233 @default.
- W1540618931 hasConceptScore W1540618931C552990157 @default.
- W1540618931 hasConceptScore W1540618931C61320498 @default.
- W1540618931 hasConceptScore W1540618931C71924100 @default.
- W1540618931 hasConceptScore W1540618931C86803240 @default.
- W1540618931 hasConceptScore W1540618931C91965660 @default.
- W1540618931 hasLocation W15406189311 @default.
- W1540618931 hasOpenAccess W1540618931 @default.
- W1540618931 hasPrimaryLocation W15406189311 @default.
- W1540618931 hasRelatedWork W1511534472 @default.
- W1540618931 hasRelatedWork W1995010744 @default.
- W1540618931 hasRelatedWork W2025848793 @default.
- W1540618931 hasRelatedWork W2074095225 @default.
- W1540618931 hasRelatedWork W2134527842 @default.
- W1540618931 hasRelatedWork W2154853819 @default.
- W1540618931 hasRelatedWork W2262739684 @default.
- W1540618931 hasRelatedWork W2418495356 @default.
- W1540618931 hasRelatedWork W2565301280 @default.
- W1540618931 hasRelatedWork W2726026710 @default.
- W1540618931 hasRelatedWork W2741717377 @default.
- W1540618931 hasRelatedWork W2743802042 @default.
- W1540618931 hasRelatedWork W2776822368 @default.
- W1540618931 hasRelatedWork W2948734763 @default.
- W1540618931 hasRelatedWork W2966418733 @default.
- W1540618931 hasRelatedWork W2983348681 @default.
- W1540618931 hasRelatedWork W3031860757 @default.
- W1540618931 hasRelatedWork W3083188116 @default.
- W1540618931 hasRelatedWork W3094852234 @default.
- W1540618931 hasRelatedWork W3137332054 @default.
- W1540618931 hasVolume "12" @default.
- W1540618931 isParatext "false" @default.
- W1540618931 isRetracted "false" @default.
- W1540618931 magId "1540618931" @default.
- W1540618931 workType "article" @default.