Matches in SemOpenAlex for { <https://semopenalex.org/work/W1540891207> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1540891207 endingPage "4278" @default.
- W1540891207 startingPage "4271" @default.
- W1540891207 abstract "Luo and Taylor (J. Virol. 64:4321-4328, 1990) have previously shown that when, during RNA-directed DNA synthesis, a retroviral reverse transcriptase comes to a halt at the end of an RNA template, the associated RNase H produces a specific oligonucleotide that contains the 5' end of that template; in those studies the length of the oligonucleotide was predominantly 17 nucleotides. We have now investigated variables that might affect the formation and length of such a terminal oligonucleotide. We found small but significant variations in the length could be caused by the choice of reaction conditions and also the sources of reverse transcriptase and RNA template. Nevertheless, the general finding in all these situations was that RNase H acted at or about 14 to 18 nucleotides from the 5' end, thereby supporting the interpretation that in the reverse transcriptase, the cleavage site for the RNase H is held at around this distance behind the DNA polymerase activity. In other words, it appears that for the intact protein, the RNase H and reverse transcriptase activities may work in a coupled or coordinate manner. We also found that more than 80% of the residual 5' oligonucleotides remained base paired to the RNA-directed DNA product. Furthermore, under certain conditions, these short RNAs could act as efficient primers for an associated DNA-directed DNA synthesis in the reverse direction." @default.
- W1540891207 created "2016-06-24" @default.
- W1540891207 creator A5037087546 @default.
- W1540891207 creator A5088766033 @default.
- W1540891207 date "1992-07-01" @default.
- W1540891207 modified "2023-10-14" @default.
- W1540891207 title "When retroviral reverse transcriptases reach the end of their RNA templates" @default.
- W1540891207 cites W1467797602 @default.
- W1540891207 cites W1492828286 @default.
- W1540891207 cites W1524887516 @default.
- W1540891207 cites W1555146480 @default.
- W1540891207 cites W1568040165 @default.
- W1540891207 cites W1571463280 @default.
- W1540891207 cites W1578991941 @default.
- W1540891207 cites W1580027475 @default.
- W1540891207 cites W1585835 @default.
- W1540891207 cites W1591988375 @default.
- W1540891207 cites W1594924045 @default.
- W1540891207 cites W1888137339 @default.
- W1540891207 cites W1970850106 @default.
- W1540891207 cites W1994777317 @default.
- W1540891207 cites W1999103713 @default.
- W1540891207 cites W2010284680 @default.
- W1540891207 cites W2028094900 @default.
- W1540891207 cites W2056935905 @default.
- W1540891207 cites W2074614836 @default.
- W1540891207 cites W2088196297 @default.
- W1540891207 cites W2129923672 @default.
- W1540891207 cites W2398460165 @default.
- W1540891207 cites W4850913 @default.
- W1540891207 doi "https://doi.org/10.1128/jvi.66.7.4271-4278.1992" @default.
- W1540891207 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/241232" @default.
- W1540891207 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1376369" @default.
- W1540891207 hasPublicationYear "1992" @default.
- W1540891207 type Work @default.
- W1540891207 sameAs 1540891207 @default.
- W1540891207 citedByCount "66" @default.
- W1540891207 countsByYear W15408912072013 @default.
- W1540891207 countsByYear W15408912072022 @default.
- W1540891207 crossrefType "journal-article" @default.
- W1540891207 hasAuthorship W1540891207A5037087546 @default.
- W1540891207 hasAuthorship W1540891207A5088766033 @default.
- W1540891207 hasBestOaLocation W15408912071 @default.
- W1540891207 hasConcept C104317684 @default.
- W1540891207 hasConcept C10879258 @default.
- W1540891207 hasConcept C129312508 @default.
- W1540891207 hasConcept C153911025 @default.
- W1540891207 hasConcept C156719811 @default.
- W1540891207 hasConcept C185581394 @default.
- W1540891207 hasConcept C2756471 @default.
- W1540891207 hasConcept C2910803801 @default.
- W1540891207 hasConcept C54355233 @default.
- W1540891207 hasConcept C552990157 @default.
- W1540891207 hasConcept C67705224 @default.
- W1540891207 hasConcept C69766542 @default.
- W1540891207 hasConcept C82381507 @default.
- W1540891207 hasConcept C86803240 @default.
- W1540891207 hasConceptScore W1540891207C104317684 @default.
- W1540891207 hasConceptScore W1540891207C10879258 @default.
- W1540891207 hasConceptScore W1540891207C129312508 @default.
- W1540891207 hasConceptScore W1540891207C153911025 @default.
- W1540891207 hasConceptScore W1540891207C156719811 @default.
- W1540891207 hasConceptScore W1540891207C185581394 @default.
- W1540891207 hasConceptScore W1540891207C2756471 @default.
- W1540891207 hasConceptScore W1540891207C2910803801 @default.
- W1540891207 hasConceptScore W1540891207C54355233 @default.
- W1540891207 hasConceptScore W1540891207C552990157 @default.
- W1540891207 hasConceptScore W1540891207C67705224 @default.
- W1540891207 hasConceptScore W1540891207C69766542 @default.
- W1540891207 hasConceptScore W1540891207C82381507 @default.
- W1540891207 hasConceptScore W1540891207C86803240 @default.
- W1540891207 hasIssue "7" @default.
- W1540891207 hasLocation W15408912071 @default.
- W1540891207 hasLocation W15408912072 @default.
- W1540891207 hasLocation W15408912073 @default.
- W1540891207 hasLocation W15408912074 @default.
- W1540891207 hasOpenAccess W1540891207 @default.
- W1540891207 hasPrimaryLocation W15408912071 @default.
- W1540891207 hasRelatedWork W1497781362 @default.
- W1540891207 hasRelatedWork W1540891207 @default.
- W1540891207 hasRelatedWork W1969533877 @default.
- W1540891207 hasRelatedWork W2010566717 @default.
- W1540891207 hasRelatedWork W2012370202 @default.
- W1540891207 hasRelatedWork W2018893626 @default.
- W1540891207 hasRelatedWork W2073436904 @default.
- W1540891207 hasRelatedWork W2086402826 @default.
- W1540891207 hasRelatedWork W2153193209 @default.
- W1540891207 hasRelatedWork W2578750663 @default.
- W1540891207 hasVolume "66" @default.
- W1540891207 isParatext "false" @default.
- W1540891207 isRetracted "false" @default.
- W1540891207 magId "1540891207" @default.
- W1540891207 workType "article" @default.