FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1541065761> ?p ?o ?g. }

• W1541065761 endingPage "573" @default.
• W1541065761 startingPage "567" @default.
• W1541065761 abstract "DM43 is a circulating dimeric antitoxin isolated from Didelphis aurita , a South American marsupial naturally immune to snake envenomation. This endogenous inhibitor binds non‐covalently to jararhagin, the main hemorrhagic metalloproteinase from Bothrops jararaca snake venom, and efficiently neutralizes its toxicity. The aim of this study was to apply mass spectrometry (MS) and surface plasmon resonance (SPR) to improve the molecular characterization of this heterocomplex. The stoichiometry of the interaction was confirmed by nanoelectrospray ionization‐quadrupole‐time‐of‐flight MS; from native solution conditions, the complex showed a molecular mass of ~94 kDa, indicating that one molecule of jararhagin (50 kDa) interacts with one monomer of DM43 (43 kDa). Although readily observed in solution, the dimeric structure of the inhibitor was barely preserved in the gas phase. This result suggests that, in contrast to the toxin–antitoxin complex, hydrophobic interactions are the primary driving force for the inhibitor dimerization. For the real‐time interaction analysis, the toxin was captured on a sensor chip derivatized with the anti‐jararhagin monoclonal antibody MAJar 2. The sensorgrams obtained after successive injections of DM43 in a concentration series were globally fitted to a simple bimolecular interaction, yielding the following kinetic rates for the DM43/jararhagin interaction: k a = 3.54 ± 0.03 × 10 4 M −1 s −1 and k d = 1.16 ± 0.07 × 10 −5 s −1 , resulting in an equilibrium dissociation constant ( K D ) of 0.33 ± 0.06 nM. Taken together, MS and SPR results show that DM43 binds to its target toxin with high affinity and constitute the first accurate quantitative study on the extent of the interaction between a natural inhibitor and a metalloproteinase toxin, with unequivocal implications for the use of this kind of molecule as template for the rational development of novel antivenom therapies. Copyright © 2012 John Wiley & Sons, Ltd." @default.
• W1541065761 created "2016-06-24" @default.
• W1541065761 creator A5003402066 @default.
• W1541065761 creator A5010027263 @default.
• W1541065761 creator A5017421418 @default.
• W1541065761 creator A5019979618 @default.
• W1541065761 creator A5039755087 @default.
• W1541065761 creator A5046521830 @default.
• W1541065761 creator A5049027923 @default.
• W1541065761 creator A5050532514 @default.
• W1541065761 creator A5063740591 @default.
• W1541065761 creator A5066094184 @default.
• W1541065761 creator A5078935473 @default.
• W1541065761 creator A5081331636 @default.
• W1541065761 creator A5085434468 @default.
• W1541065761 date "2012-05-01" @default.
• W1541065761 modified "2023-10-18" @default.
• W1541065761 title "The interaction of the antitoxin DM43 with a snake venom metalloproteinase analyzed by mass spectrometry and surface plasmon resonance" @default.
• W1541065761 cites W1532085934 @default.
• W1541065761 cites W1549345436 @default.
• W1541065761 cites W1565563859 @default.
• W1541065761 cites W1569210877 @default.
• W1541065761 cites W1926568554 @default.
• W1541065761 cites W1965241501 @default.
• W1541065761 cites W1975599463 @default.
• W1541065761 cites W1984267713 @default.
• W1541065761 cites W1994859763 @default.
• W1541065761 cites W1995516187 @default.
• W1541065761 cites W1999604004 @default.
• W1541065761 cites W2000063727 @default.
• W1541065761 cites W2001827418 @default.
• W1541065761 cites W2009742493 @default.
• W1541065761 cites W2031772330 @default.
• W1541065761 cites W2033111976 @default.
• W1541065761 cites W2035546135 @default.
• W1541065761 cites W2042818895 @default.
• W1541065761 cites W2051583331 @default.
• W1541065761 cites W2068722030 @default.
• W1541065761 cites W2071884490 @default.
• W1541065761 cites W2077008508 @default.
• W1541065761 cites W2078302553 @default.
• W1541065761 cites W2078341502 @default.
• W1541065761 cites W2082305250 @default.
• W1541065761 cites W2088782278 @default.
• W1541065761 cites W2101348082 @default.
• W1541065761 cites W2101420231 @default.
• W1541065761 cites W2103787428 @default.
• W1541065761 cites W2109081265 @default.
• W1541065761 cites W2122870421 @default.
• W1541065761 cites W2129733824 @default.
• W1541065761 cites W2137836801 @default.
• W1541065761 cites W2143153462 @default.
• W1541065761 cites W2155602568 @default.
• W1541065761 cites W2160794341 @default.
• W1541065761 cites W2162298868 @default.
• W1541065761 cites W2168771275 @default.
• W1541065761 cites W23530191 @default.
• W1541065761 cites W36676292 @default.
• W1541065761 cites W4211220845 @default.
• W1541065761 doi "https://doi.org/10.1002/jms.2990" @default.
• W1541065761 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22549991" @default.
• W1541065761 hasPublicationYear "2012" @default.
• W1541065761 type Work @default.
• W1541065761 sameAs 1541065761 @default.
• W1541065761 citedByCount "11" @default.
• W1541065761 countsByYear W15410657612012 @default.
• W1541065761 countsByYear W15410657612014 @default.
• W1541065761 countsByYear W15410657612015 @default.
• W1541065761 countsByYear W15410657612016 @default.
• W1541065761 countsByYear W15410657612017 @default.
• W1541065761 countsByYear W15410657612018 @default.
• W1541065761 countsByYear W15410657612021 @default.
• W1541065761 countsByYear W15410657612022 @default.
• W1541065761 countsByYear W15410657612023 @default.
• W1541065761 crossrefType "journal-article" @default.
• W1541065761 hasAuthorship W1541065761A5003402066 @default.
• W1541065761 hasAuthorship W1541065761A5010027263 @default.
• W1541065761 hasAuthorship W1541065761A5017421418 @default.
• W1541065761 hasAuthorship W1541065761A5019979618 @default.
• W1541065761 hasAuthorship W1541065761A5039755087 @default.
• W1541065761 hasAuthorship W1541065761A5046521830 @default.
• W1541065761 hasAuthorship W1541065761A5049027923 @default.
• W1541065761 hasAuthorship W1541065761A5050532514 @default.
• W1541065761 hasAuthorship W1541065761A5063740591 @default.
• W1541065761 hasAuthorship W1541065761A5066094184 @default.
• W1541065761 hasAuthorship W1541065761A5078935473 @default.
• W1541065761 hasAuthorship W1541065761A5081331636 @default.
• W1541065761 hasAuthorship W1541065761A5085434468 @default.
• W1541065761 hasConcept C106847996 @default.
• W1541065761 hasConcept C12554922 @default.
• W1541065761 hasConcept C155672457 @default.
• W1541065761 hasConcept C162356407 @default.
• W1541065761 hasConcept C170493617 @default.
• W1541065761 hasConcept C171250308 @default.
• W1541065761 hasConcept C185592680 @default.
• W1541065761 hasConcept C192562407 @default.
• W1541065761 hasConcept C2777367657 @default.
• W1541065761 hasConcept C2778973234 @default.

• next