FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1541408984> ?p ?o ?g. }

• W1541408984 endingPage "7982" @default.
• W1541408984 startingPage "7975" @default.
• W1541408984 abstract "Adipocytes were incubated with [32P]phosphate to achieve steady state labeling of glycogen synthase. The enzyme was then rapidly immunoprecipitated and subjected to electrophoresis on polyacrylamide slab gels in the presence of sodium dodecyl sulfate. The 32P-labeled glycogen synthase had an apparent molecular weight ( Mapp ) equal to 90,000. All of the [32P]phosphate could be recovered in two cyanogen bromide fragments. The larger fragment, CB-2 ( Mapp = 28,000), contained about five times more [32P]phosphate than the smaller fragment, CB-1 ( Mapp = 15,500). Insulin increased the activity ratio (-glucose-6-P/+glucose-6-P) of glycogen synthase from 0.12 to 0.26, but did not decrease the amount of [32P]phosphate in the enzyme. However, insulin promoted the formation of species of CB-2 of lower Mapp , suggesting dephosphorylation of sites that affected the electrophoretic mobility of the fragment. Glucose did not affect the mobility of CB-2, but slightly increased the activity ratio and decreased the [32P] phosphate by approximately 20%. With insulin plus glucose, the increase in activity ratio was much greater than the additive effects of either agent alone. The combination decreased the [32P]phosphate in each cyanogen bromide fragment by approximately 60%, indicating that the synergistic activation was due to enhanced dephosphorylation of multiple sites. 2-Deoxyglucose also promoted dephosphorylation of glycogen synthase, decreasing the 32P content of CB-1 and CB-2 by approximately 40% each. 3-O-Methylglucose was without effect. The results presented suggest that the activation of glycogen synthase by insulin via a glucose transport-dependent pathway may involve increased intracellular glucose-6-P which promotes dephosphorylation of sites in both CB-1 and CB-2. Activation by a glucose transport-independent pathway appears to be confined to sites located in CB-2." @default.
• W1541408984 created "2016-06-24" @default.
• W1541408984 creator A5022178753 @default.
• W1541408984 creator A5027573323 @default.
• W1541408984 date "1984-06-01" @default.
• W1541408984 modified "2023-09-30" @default.
• W1541408984 title "Activation of glycogen synthase by insulin in rat adipocytes. Evidence of hormonal stimulation of multisite dephosphorylation by glucose transport-dependent and -independent pathways." @default.
• W1541408984 cites W1485191693 @default.
• W1541408984 cites W1486325186 @default.
• W1541408984 cites W1492556959 @default.
• W1541408984 cites W1497588826 @default.
• W1541408984 cites W1498907057 @default.
• W1541408984 cites W1505976463 @default.
• W1541408984 cites W1508552403 @default.
• W1541408984 cites W1515345850 @default.
• W1541408984 cites W1516572497 @default.
• W1541408984 cites W1528048308 @default.
• W1541408984 cites W1534015211 @default.
• W1541408984 cites W1535002454 @default.
• W1541408984 cites W1535112631 @default.
• W1541408984 cites W1537611567 @default.
• W1541408984 cites W1558592232 @default.
• W1541408984 cites W1558665550 @default.
• W1541408984 cites W1561878114 @default.
• W1541408984 cites W1562094939 @default.
• W1541408984 cites W1562140236 @default.
• W1541408984 cites W1563594465 @default.
• W1541408984 cites W1570881500 @default.
• W1541408984 cites W1572145449 @default.
• W1541408984 cites W1573034460 @default.
• W1541408984 cites W1578220881 @default.
• W1541408984 cites W1589137921 @default.
• W1541408984 cites W1590947913 @default.
• W1541408984 cites W1597813488 @default.
• W1541408984 cites W1599961357 @default.
• W1541408984 cites W1600791799 @default.
• W1541408984 cites W1608447403 @default.
• W1541408984 cites W1625660792 @default.
• W1541408984 cites W164548000 @default.
• W1541408984 cites W1664108429 @default.
• W1541408984 cites W1742701470 @default.
• W1541408984 cites W1895996242 @default.
• W1541408984 cites W1965371391 @default.
• W1541408984 cites W1969637582 @default.
• W1541408984 cites W1970885444 @default.
• W1541408984 cites W1973223525 @default.
• W1541408984 cites W1973395730 @default.
• W1541408984 cites W1983854256 @default.
• W1541408984 cites W1983940749 @default.
• W1541408984 cites W1987654456 @default.
• W1541408984 cites W2001866120 @default.
• W1541408984 cites W2002744790 @default.
• W1541408984 cites W2012282437 @default.
• W1541408984 cites W2013624469 @default.
• W1541408984 cites W2016133866 @default.
• W1541408984 cites W2018275786 @default.
• W1541408984 cites W2018709107 @default.
• W1541408984 cites W2025634505 @default.
• W1541408984 cites W2025758232 @default.
• W1541408984 cites W2031550040 @default.
• W1541408984 cites W2040770143 @default.
• W1541408984 cites W2042365395 @default.
• W1541408984 cites W2062392784 @default.
• W1541408984 cites W2071618910 @default.
• W1541408984 cites W2085119576 @default.
• W1541408984 cites W2085839645 @default.
• W1541408984 cites W2087461901 @default.
• W1541408984 cites W2091337057 @default.
• W1541408984 cites W2094170912 @default.
• W1541408984 cites W2100837269 @default.
• W1541408984 cites W2105573423 @default.
• W1541408984 cites W2143873575 @default.
• W1541408984 cites W2170694448 @default.
• W1541408984 cites W2181515955 @default.
• W1541408984 cites W2301599923 @default.
• W1541408984 cites W2396020013 @default.
• W1541408984 cites W2809381039 @default.
• W1541408984 cites W3111249283 @default.
• W1541408984 cites W4255234850 @default.
• W1541408984 cites W4300044664 @default.
• W1541408984 cites W4323913119 @default.
• W1541408984 cites W69093701 @default.
• W1541408984 doi "https://doi.org/10.1016/s0021-9258(17)42888-2" @default.
• W1541408984 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6429136" @default.
• W1541408984 hasPublicationYear "1984" @default.
• W1541408984 type Work @default.
• W1541408984 sameAs 1541408984 @default.
• W1541408984 citedByCount "37" @default.
• W1541408984 crossrefType "journal-article" @default.
• W1541408984 hasAuthorship W1541408984A5022178753 @default.
• W1541408984 hasAuthorship W1541408984A5027573323 @default.
• W1541408984 hasBestOaLocation W15414089841 @default.
• W1541408984 hasConcept C109384507 @default.
• W1541408984 hasConcept C112243037 @default.
• W1541408984 hasConcept C11960822 @default.
• W1541408984 hasConcept C126322002 @default.
• W1541408984 hasConcept C134018914 @default.
• W1541408984 hasConcept C178666793 @default.

• next