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- W1541651640 abstract "The aim of the research was to identify the polymorphisms of HSP-70 gene of kampong, Arabic, and commercial chickens. The study used whole blood samples taken from the branchial vein at the age of 12 weeks from a group of chickens that were kept since DOC. Kampong chickens DOC were obtained by hatching the eggs that were collected from lowlands, medium and high altitude areas in Lombok island, while the Arabic chickens DOC were obtained from farmer. DOC of commercial chickens were purchased at the poultry shop. The genotyping results using PCR-SSCP analysis showed that kampong and arabic chickens were polimorphic, while commercial chickens were classified as monomorphic. Kampong chickens had seven genotypes of HSP-70, namely AA, AB, AC, CC, AD, DD, and BC. The AD genotypes had the highest frequency, whereas in arabic chickens had six genotypes, namely AA, AB, AC, CC, AD and BC, which AC genotype had the highest frequencies. However, the commercial chickens contain only one genotype which was DD. There were three polymorphic sites found in amplificated area, namely A G mutation at site 617, A G mutation at site 628, G C mutation at site 646, C T mutation at site 661, A G mutation at site 699, G C mutation at site 754, and A G mutation at site 792. Mutations at sites 628, 646 and 661 were considered as silent mutation. HSP-70 gene in kampung and arabic chicken population in this study was in equilibrium. The value of Ho, He, and PIC of kampung and arabic chickens were not different." @default.
- W1541651640 created "2016-06-24" @default.
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- W1541651640 date "2013-11-25" @default.
- W1541651640 modified "2023-09-23" @default.
- W1541651640 title "Keragaman Gen Heat Shock Protein 70 pada Ayam Kampung, Ayam Arab, dan Ayam Ras (POLYMORPHISMS OF THE HEAT SHOCK PROTEIN 70 GENE IN KAMPONG, ARABIC, AND COMMERCIAL CHICKENS)" @default.
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