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- W1541782648 abstract "Reovirus RNA is a very effective template in RNA synthesis catalyzed by RNA polymerase from Escherichia coli B. With all four triphosphates present in the reaction mixture, there is synthesis not only of an RNA product with a base composition similar to that of the primer RNA, but also of polyadenylate and polyuridylate. Pancreatic ribonuclease rendered acid-soluble 50% of the [14C]CMP-labeled, reovirus RNA-primed product obtained after 4 minutes of synthesis. Reovirus RNA-primed incorporation of cytidine monophosphate into acid-insoluble product was inhibited 70% by 2 μg/ml. of actinomycin D. Reovirus RNA caused only a minimal depression in the absorption maximum of actinomycin D at 440 mμ, without a shift of the maximum to longer wavelengths." @default.
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- W1541782648 date "1964-07-01" @default.
- W1541782648 modified "2023-09-26" @default.
- W1541782648 title "Enzymic synthesis of RNA with reovirus RNA as template" @default.
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- W1541782648 doi "https://doi.org/10.1016/s0022-2836(64)80100-5" @default.
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