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- W1542470963 abstract "Pieces of ventral prostate from adult C3H mice were cultivated in organ culture for 3 weeks. One group served as a control; another was treated for I week with methylcholanthrene or 9,1O-dimethyl-1,2-benzanthracene and for 2 additional weeks in normal medium. The pieces were pooled and dispersed with pronase into individual cells that were plated as cell cultures. The control cultures invariably died. The treated cells formed permanent lines that, on subcutaneous injection of from 1 to 2 X 10^6 cells into adult, unconditioned, male C3H mice, produced progressively growing, transplantable tumors. The tumors were mostly sarcomas, but included two anaplastic carcinomas. Malignant transformation in vitro has thus been achieved with carcinogenic hydrocarbons in this system." @default.
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- W1542470963 date "1967-01-13" @default.
- W1542470963 modified "2023-09-26" @default.
- W1542470963 title "Malignant Transformation in vitro by Carcinogenic Hydrocarbons" @default.
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- W1542470963 doi "https://doi.org/10.1126/science.217" @default.
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