FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1542486621> ?p ?o ?g. }

• W1542486621 abstract "Phosphorylation of phospholipase C-delta(1) (PLC-delta(1)) in vitro and in vivo was investigated. Of the serine/threonine kinases tested, protein kinase C (PKC) phosphorylated the serine residue(s) of bacterially expressed PLC-delta(1) most potently. It was also demonstrated that PLC-delta(1) directly bound PKC-alpha via its pleckstrin homology (PH) domain. Using deletion mutants of PLC-delta(1) and synthetic peptides, Ser35 in the PH domain was defined as the PKC mediated in vitro phosphorylation site of PLC-delta(1). In vitro phosphorylation of PLC-delta(1) by PKC stimulated [(3)H]PtdIns(4,5)P(2) hydrolyzing activity and [(3)H]Ins(1,4,5)P(3)-binding of the PLC-delta(1). On the other hand, endogenous PLC-delta(1) was constitutively phosphorylated and phosphoamino acid analysis revealed that major phosphorylation sites were threonine residues in quiescent cells. The phosphorylation level and the species of phosphoamino acid were not changed by various stimuli such as PMA, EGF, NGF, and forskolin. Using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, we determined that Thr209 of PLC-delta(1) is one of the constitutively phosphorylated sites in quiescent cells. The PLC activity was potentiated when constitutively phosphorylated PLC-delta(1) was dephosphorylated by endogenous phosphatase(s) in vitro. Additionally, coexpression with PKC-alpha reduced serine phosphorylation of PLC-delta(1) detected by an anti-phosphoserine antibody and PLC-delta(1)-dependent basal production of inositol phosphates in NIH-3T3 cells, suggesting PKC-alpha activates phosphatase or inactivates another kinase involved in PLC-delta(1) serine phosphorylation to modulate the PLC-delta(1) activity in vivo. Taken together, these results suggest that PLC-delta(1) has multiple phosphorylation sites and phosphorylation status of PLC-delta(1) regulates its activity positively or negatively depends on the phosphorylation sites." @default.
• W1542486621 created "2016-06-24" @default.
• W1542486621 creator A5016261214 @default.
• W1542486621 creator A5016285662 @default.
• W1542486621 creator A5032289839 @default.
• W1542486621 creator A5063560544 @default.
• W1542486621 creator A5068359295 @default.
• W1542486621 creator A5072018457 @default.
• W1542486621 creator A5086899824 @default.
• W1542486621 date "2009-10-15" @default.
• W1542486621 modified "2023-09-27" @default.
• W1542486621 title "Phosphorylation of Phospholipase C-δ1Regulates its Enzymatic Activity" @default.
• W1542486621 cites W10026949 @default.
• W1542486621 cites W1869540660 @default.
• W1542486621 cites W1967202041 @default.
• W1542486621 cites W1969893193 @default.
• W1542486621 cites W1970692622 @default.
• W1542486621 cites W1977239487 @default.
• W1542486621 cites W1979817897 @default.
• W1542486621 cites W1980166477 @default.
• W1542486621 cites W1981182586 @default.
• W1542486621 cites W1985916633 @default.
• W1542486621 cites W1986861517 @default.
• W1542486621 cites W1991062453 @default.
• W1542486621 cites W2003190738 @default.
• W1542486621 cites W2006416359 @default.
• W1542486621 cites W2011915496 @default.
• W1542486621 cites W2016441216 @default.
• W1542486621 cites W2030579890 @default.
• W1542486621 cites W2030856691 @default.
• W1542486621 cites W2032786799 @default.
• W1542486621 cites W2037434894 @default.
• W1542486621 cites W2038612592 @default.
• W1542486621 cites W2041121472 @default.
• W1542486621 cites W2045291272 @default.
• W1542486621 cites W2056797323 @default.
• W1542486621 cites W2057227495 @default.
• W1542486621 cites W2060444233 @default.
• W1542486621 cites W2069699841 @default.
• W1542486621 cites W2080115585 @default.
• W1542486621 cites W2083705213 @default.
• W1542486621 cites W2084009771 @default.
• W1542486621 cites W2086524338 @default.
• W1542486621 cites W2088347772 @default.
• W1542486621 cites W2092249847 @default.
• W1542486621 cites W2096549360 @default.
• W1542486621 cites W2100117790 @default.
• W1542486621 cites W2101187809 @default.
• W1542486621 cites W2103609214 @default.
• W1542486621 cites W2104982236 @default.
• W1542486621 cites W2109252978 @default.
• W1542486621 cites W2116903886 @default.
• W1542486621 cites W2121808401 @default.
• W1542486621 cites W2129366439 @default.
• W1542486621 cites W2166856535 @default.
• W1542486621 cites W2171733163 @default.
• W1542486621 cites W2189033227 @default.
• W1542486621 cites W2400113837 @default.
• W1542486621 cites W986378079 @default.
• W1542486621 doi "https://doi.org/10.1002/jcb.22297" @default.
• W1542486621 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19681039" @default.
• W1542486621 hasPublicationYear "2009" @default.
• W1542486621 type Work @default.
• W1542486621 sameAs 1542486621 @default.
• W1542486621 citedByCount "11" @default.
• W1542486621 countsByYear W15424866212012 @default.
• W1542486621 countsByYear W15424866212013 @default.
• W1542486621 countsByYear W15424866212014 @default.
• W1542486621 countsByYear W15424866212015 @default.
• W1542486621 countsByYear W15424866212019 @default.
• W1542486621 countsByYear W15424866212021 @default.
• W1542486621 countsByYear W15424866212022 @default.
• W1542486621 crossrefType "journal-article" @default.
• W1542486621 hasAuthorship W1542486621A5016261214 @default.
• W1542486621 hasAuthorship W1542486621A5016285662 @default.
• W1542486621 hasAuthorship W1542486621A5032289839 @default.
• W1542486621 hasAuthorship W1542486621A5063560544 @default.
• W1542486621 hasAuthorship W1542486621A5068359295 @default.
• W1542486621 hasAuthorship W1542486621A5072018457 @default.
• W1542486621 hasAuthorship W1542486621A5086899824 @default.
• W1542486621 hasConcept C11960822 @default.
• W1542486621 hasConcept C153911025 @default.
• W1542486621 hasConcept C178666793 @default.
• W1542486621 hasConcept C181912034 @default.
• W1542486621 hasConcept C184235292 @default.
• W1542486621 hasConcept C185592680 @default.
• W1542486621 hasConcept C195794163 @default.
• W1542486621 hasConcept C2775880066 @default.
• W1542486621 hasConcept C2776414213 @default.
• W1542486621 hasConcept C2778597717 @default.
• W1542486621 hasConcept C2779933727 @default.
• W1542486621 hasConcept C2780307904 @default.
• W1542486621 hasConcept C49658373 @default.
• W1542486621 hasConcept C55493867 @default.
• W1542486621 hasConcept C62478195 @default.
• W1542486621 hasConcept C86803240 @default.
• W1542486621 hasConceptScore W1542486621C11960822 @default.
• W1542486621 hasConceptScore W1542486621C153911025 @default.
• W1542486621 hasConceptScore W1542486621C178666793 @default.
• W1542486621 hasConceptScore W1542486621C181912034 @default.

• next