FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1542487231> ?p ?o ?g. }

• W1542487231 endingPage "3230" @default.
• W1542487231 startingPage "3215" @default.
• W1542487231 abstract "Key points A planar nanocrystalline diamond array with nine ultra‐microelectrodes (9‐Ch NCD‐UMEA) has been designed for high spatial resolution of amperometric recordings in single chromaffin cells. The 9‐Ch NCD‐UMEA operates in voltammetric and amperometric mode to reveal low doses of adrenaline, dopamine and serotonin. The lowest detectable concentration of adrenaline is ∼5 μ m . Using mouse and bovine chromaffin cells, single quantal exocytotic events are recorded from nine microareas of 12–27 μm 2 . We found an excellent correspondence with recordings from the cell apex using carbon fibre electrodes. In the bovine, secretion is heterogeneous. There are areas of high and medium activity covering 54% of the cell surface and areas of low and no activity covering the remainder. The ‘non‐active zones’ (silent) cover 24% of the cell surface and persist for minutes as the ‘active zones’. The 9‐Ch NCD‐UMEA brings new insights into the spatial mapping of secretory sites in chromaffin cells. Abstract Here we describe the ability of a high‐density diamond microelectrode array targeted to resolve multi‐site detection of fast exocytotic events from single cells. The array consists of nine boron‐doped nanocrystalline diamond ultra‐microelectrodes (9‐Ch NCD‐UMEA) radially distributed within a circular area of the dimensions of a single cell. The device can be operated in voltammetric or chronoamperometric configuration. Sensitivity to catecholamines, tested by dose–response calibrations, set the lowest detectable concentration of adrenaline to ∼5 μ m . Catecholamine release from bovine or mouse chromaffin cells could be triggered by electrical stimulation or external KCl‐enriched solutions. Spikes detected from the cell apex using carbon fibre microelectrodes showed an excellent correspondence with events measured at the bottom of the cell by the 9‐Ch NCD‐UMEA, confirming the ability of the array to resolve single quantal secretory events. Subcellular localization of exocytosis was provided by assigning each quantal event to one of the nine channels based on its location. The resulting mapping highlights the heterogeneous distribution of secretory activity in cell microdomains of 12–27 μm 2 . In bovine chromaffin cells, secretion was highly heterogeneous with zones of high and medium activity in 54% of the cell surface and zones of low or no activity in the remainder. The ‘non‐active’ (‘silent’) zones covered 24% of the total and persisted for 6–8 min, indicating stable location. The 9‐Ch NCD‐UMEA therefore appears suitable for investigating the microdomain organization of neurosecretion with high spatial resolution." @default.
• W1542487231 created "2016-06-24" @default.
• W1542487231 creator A5000101332 @default.
• W1542487231 creator A5005213954 @default.
• W1542487231 creator A5017792406 @default.
• W1542487231 creator A5031941235 @default.
• W1542487231 creator A5059811108 @default.
• W1542487231 creator A5060746359 @default.
• W1542487231 creator A5067920458 @default.
• W1542487231 date "2014-06-27" @default.
• W1542487231 modified "2023-09-30" @default.
• W1542487231 title "Heterogeneous distribution of exocytotic microdomains in adrenal chromaffin cells resolved by high-density diamond ultra-microelectrode arrays" @default.
• W1542487231 cites W1492572803 @default.
• W1542487231 cites W1531962736 @default.
• W1542487231 cites W1873000357 @default.
• W1542487231 cites W1968392714 @default.
• W1542487231 cites W1970200112 @default.
• W1542487231 cites W1970801309 @default.
• W1542487231 cites W1972637332 @default.
• W1542487231 cites W1975246201 @default.
• W1542487231 cites W1984737463 @default.
• W1542487231 cites W1987239452 @default.
• W1542487231 cites W1987368066 @default.
• W1542487231 cites W1988712581 @default.
• W1542487231 cites W1993723732 @default.
• W1542487231 cites W1994731139 @default.
• W1542487231 cites W1994883195 @default.
• W1542487231 cites W2006239536 @default.
• W1542487231 cites W2006695726 @default.
• W1542487231 cites W2007533004 @default.
• W1542487231 cites W2013345595 @default.
• W1542487231 cites W2017757859 @default.
• W1542487231 cites W2017847991 @default.
• W1542487231 cites W2020002854 @default.
• W1542487231 cites W2021633739 @default.
• W1542487231 cites W2025795325 @default.
• W1542487231 cites W2025961643 @default.
• W1542487231 cites W2029261483 @default.
• W1542487231 cites W2031698980 @default.
• W1542487231 cites W2032237431 @default.
• W1542487231 cites W2033067408 @default.
• W1542487231 cites W2038299843 @default.
• W1542487231 cites W2038665347 @default.
• W1542487231 cites W2046108016 @default.
• W1542487231 cites W2049154146 @default.
• W1542487231 cites W2067970581 @default.
• W1542487231 cites W2071848758 @default.
• W1542487231 cites W2072514286 @default.
• W1542487231 cites W2075551783 @default.
• W1542487231 cites W2080954023 @default.
• W1542487231 cites W2083810544 @default.
• W1542487231 cites W2095095727 @default.
• W1542487231 cites W2098390025 @default.
• W1542487231 cites W2106326175 @default.
• W1542487231 cites W2113154080 @default.
• W1542487231 cites W2137229430 @default.
• W1542487231 cites W2137639709 @default.
• W1542487231 cites W2160921509 @default.
• W1542487231 cites W2167589820 @default.
• W1542487231 cites W4244054492 @default.
• W1542487231 doi "https://doi.org/10.1113/jphysiol.2014.274951" @default.
• W1542487231 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4146371" @default.
• W1542487231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24879870" @default.
• W1542487231 hasPublicationYear "2014" @default.
• W1542487231 type Work @default.
• W1542487231 sameAs 1542487231 @default.
• W1542487231 citedByCount "26" @default.
• W1542487231 countsByYear W15424872312014 @default.
• W1542487231 countsByYear W15424872312015 @default.
• W1542487231 countsByYear W15424872312016 @default.
• W1542487231 countsByYear W15424872312017 @default.
• W1542487231 countsByYear W15424872312018 @default.
• W1542487231 countsByYear W15424872312019 @default.
• W1542487231 countsByYear W15424872312020 @default.
• W1542487231 countsByYear W15424872312021 @default.
• W1542487231 countsByYear W15424872312022 @default.
• W1542487231 crossrefType "journal-article" @default.
• W1542487231 hasAuthorship W1542487231A5000101332 @default.
• W1542487231 hasAuthorship W1542487231A5005213954 @default.
• W1542487231 hasAuthorship W1542487231A5017792406 @default.
• W1542487231 hasAuthorship W1542487231A5031941235 @default.
• W1542487231 hasAuthorship W1542487231A5059811108 @default.
• W1542487231 hasAuthorship W1542487231A5060746359 @default.
• W1542487231 hasAuthorship W1542487231A5067920458 @default.
• W1542487231 hasBestOaLocation W15424872311 @default.
• W1542487231 hasConcept C111670793 @default.
• W1542487231 hasConcept C12554922 @default.
• W1542487231 hasConcept C134018914 @default.
• W1542487231 hasConcept C139369640 @default.
• W1542487231 hasConcept C147789679 @default.
• W1542487231 hasConcept C17525397 @default.
• W1542487231 hasConcept C178790620 @default.
• W1542487231 hasConcept C185592680 @default.
• W1542487231 hasConcept C2775894120 @default.
• W1542487231 hasConcept C2776921476 @default.
• W1542487231 hasConcept C52859227 @default.
• W1542487231 hasConcept C86803240 @default.
• W1542487231 hasConcept C95967509 @default.

• next