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- W1542612014 abstract "This chapter describes bicistronic mammalian expression vector, pHaMDR-DHFR that is used to study the effect of mutations introduced into the human multidrug resistant ( MDR)1 gene. Mutagenesis is one of the most common approaches employed in the structure–function study of proteins both in somatic cells 1,2 and in germ cells. To analyze the function of the mutagenized gene, it is generally subcloned into an expression vector that usually has two different promoters for the gene of interest and the selective marker. Studies of the internal ribosomal entry site (IRES) have made it possible to develop a bicistronic or polycistronic vector in which a fusion transcript can be obtained from two or more genes linked together by one or more IRES sequences. The second gene downstream from the IRES guarantees the expression of the first gene." @default.
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- W1542612014 date "1998-01-01" @default.
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- W1542612014 title "[34] pHaMDR-DHFR bicistronic expression system for mutational analysis of P-glycoprotein" @default.
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- W1542612014 doi "https://doi.org/10.1016/s0076-6879(98)92036-5" @default.
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