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- W1542794145 abstract "Publisher Summary As with any separation technique, the power of capillary electrophoresis (CE), in macromolecular characterization, often depends on the nature of the detector. This chapter discusses the instrumentation, methods, and advantages of the combination of this high resolution separation technique, with equally powerful mass spectrometric (MS) methods. This chapter discusses the methods, experimental considerations, and selected applications for CE-MS interfacing methods, based on electrospray ionization (ESI). It emphasizes CE separations, by free-solution electrophoresis— that is, capillary zone electrophoresis (CZE) and, to a lesser extent, capillary isotachophoresis (CITP). This chapter describes the explosive growth in ESI applications, the increasing availability of such instrumentation, the compatibility with MS methods, capable of providing extensive structural information, and the belief that the ESI interface is currently the method of choice for CE-MS, owing to its sensitivity, versatility, and ease of implementation. Finally, describes clearly the current status of CE-MS, a highly promising combination, but still somewhat limited, by the sensitivity and scan-speed constraints of conventional quadrupole mass spectrometers. It shows some of the steps that can be taken to mitigate the limitations of conventional mass spectrometers and describes new instrumental approaches that should allow the full potential of the CE-MS combination to be more fully realized." @default.
- W1542794145 created "2016-06-24" @default.
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- W1542794145 date "1996-01-01" @default.
- W1542794145 modified "2023-10-10" @default.
- W1542794145 title "[19] Capillary electrophoresis-mass spectrometry" @default.
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- W1542794145 doi "https://doi.org/10.1016/s0076-6879(96)71021-2" @default.
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