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- W1542881184 abstract "The mechanism of inactivation of papain (EC 3.4.4.10) by hydroxylamine was investigated. The inactivation was pH-dependent and the rate was rapid at alkaline pH. Nitrogen gas bubbling through the reaction mixture or addition of radical scavengers to the reaction mixture largely prevented the inactivation. The inactivation was accompanied by a decrease of the essential sulfhydryl group of papain. Treatment of inactivated papain with 2-mercaptoethanol led to partial reactivation. The inactivated papain was judged not to be dimeric on the basis of molecular weight determination.Based on these results, it is likely that hydroxylamine inactivates papain indirectly by reacting with O2 to produce some reactive species which modifies the essential sulfhydryl group of the enzyme." @default.
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- W1542881184 date "1990-01-01" @default.
- W1542881184 modified "2023-09-26" @default.
- W1542881184 title "On the mechanism of inactivation of papain by hydroxylamine." @default.
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- W1542881184 doi "https://doi.org/10.1248/cpb.38.546" @default.
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