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- W154322678 abstract "The in vitro establishment of a rapidly growing, embryogenic and friablecallus and suspension cell lines of 27 local indica rice (Oryza sativa) cultivarswere studied. The regeneration capacity of selected cultivars also observed.Differences in culture conditions, growth rate and plant hormone applicationswere examined to determine the optimal responses. Rice callus was induced fromimmature seeds on basal MS solid medium supplemented with 10 µM 2,4-D andfinely dispersed cell suspension cultures were initiated from the callus using B5basal liquid medium consisted of 10 µM 2,4-D. These callus and suspensionswere maintained in similar medium, respectively.Basal MS solid medium supplemented with 25 µM BA and 5 µM lAAwas most effective for obtaining regenerated plantlets from callus. For cellsuspension, regeneration was obtained by using basal MS solid medium with 25µM BA and 2.5 µM NAA. Out of 27 cultivars investigated, callus of 16 cultivars and cell suspensions of 11 cultivars were suscessfully regenerated. Cultivar PutehPerak exhibited good response throughout the experiments.Radioactivity study was carried out as a confirmation of2,4-D taken upby the callus and suspension cells. During the stressing and selecting stages,2,4-D was observed to be taken up by the rice callus and suspension cells.In the selection and toxicity studies, 2,4-D-tolerant callus cultivar PutehPerak was selected in MS solid media at 400, 600 and 800 µM 2,4-Dconcentration while tolerant cell-suspension in basal B5 liquid media containing200 and 400 µM 2,4-D. Both instantaneous and gradual stressing method werecarried out. The tolerant callus and cell-suspension were isolated andmaintained." @default.
- W154322678 created "2016-06-24" @default.
- W154322678 creator A5018000338 @default.
- W154322678 date "1998-09-01" @default.
- W154322678 modified "2023-09-26" @default.
- W154322678 title "In Vitro Selection, Regeneration and Herbicide Tolerant Callus and Suspensions Cell Culture of Rice (Oryza Sativa L.)" @default.
- W154322678 hasPublicationYear "1998" @default.
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