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- W1543251339 abstract "This chapter describes simplifying the fragment condensation semisynthesis of protein analogs. The goal of the chapter is to establish a set of principles to govern the choice of secondary and tertiary structural locales for the introduction of novel cleavage and religation sites. The chapter discusses the ability to predict and create sites for facile peptide condensation by the autocatalytic fragment religation method. This permits the engineering by semisynthesis of yeast cytochrome c and, potentially, many other proteins. Site-directed mutagenesis can be used to create mutants with shuffled methionine residues. Despite the wide variety of secondary structural locations chosen, mutations at the selected sites caused no major disruptions of protein structure or function. Differences in susceptibility to cyanogen bromide (CNBr) cleavage of some sites are apparent. Autocatalytic relegation can occur at many types of site, giving functional products. However, efficiency is greater when the peptide bond to be formed is surface-exposed than when it is buried." @default.
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- W1543251339 date "1996-01-01" @default.
- W1543251339 modified "2023-10-15" @default.
- W1543251339 title "Simplifying the fragment condensation semisynthesis of protein analogs" @default.
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- W1543251339 doi "https://doi.org/10.1016/s1080-8914(96)80053-6" @default.
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