FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1543294597> ?p ?o ?g. }

• W1543294597 endingPage "252" @default.
• W1543294597 startingPage "241" @default.
• W1543294597 abstract "Abstract Transcription of the Ig κ light chain gene is controlled in part by the 3′ κ enhancer. Two of the proteins that bind to the 3′ enhancer, PU.1 and Pip, show tissue-restricted expression and may be responsible for the tissue specificity of 3′ enhancer activity. PU.1 alone can bind to DNA; however, Pip cannot bind to its 3′ enhancer site in electrophoretic mobility shift assays, unless recruited by PU.1. Previously, we showed that the PU.1 PEST domain (rich in the amino acids proline, glutamate, serine, and threonine; sequences 118–160) is necessary for Pip recruitment to DNA. Here we used detailed mutagenic analyzes of PU.1 to more precisely identify sequences required for Pip recruitment by electrophoretic mobility shift assay. We found that mutation of three segments within the PU.1 PEST domain (118–125, 133–139, and 141–147) modulated the efficiency of Pip recruitment, while mutation of sequences between residues 88–118 and 154–168 had no effect. Interestingly, we found that the PU.1 ETS domain (residues 170 to 255) is both necessary and sufficient for Pip interaction in solution and that other ETS domain proteins can physically interact with Pip as well. Our results suggest that Pip recruitment to DNA by PU.1 occurs via a two-step mechanism. First, a physical interaction that is not sufficient to recruit Pip occurs via the PU.1 ETS domain. Second, a conformational change in the PU.1 PEST domain, apparently mediated by serine phosphorylation, induces a conformational change in Pip enabling it to bind to DNA. We also show that the PU.1 PEST domain does not target PU.1 for rapid turnover." @default.
• W1543294597 created "2016-06-24" @default.
• W1543294597 creator A5040547622 @default.
• W1543294597 creator A5074237345 @default.
• W1543294597 date "1998-01-01" @default.
• W1543294597 modified "2023-10-18" @default.
• W1543294597 title "A Two-Step Mechanism for Recruitment of Pip by PU.1" @default.
• W1543294597 cites W1479858937 @default.
• W1543294597 cites W1560430223 @default.
• W1543294597 cites W1605452274 @default.
• W1543294597 cites W1764615552 @default.
• W1543294597 cites W1871038 @default.
• W1543294597 cites W1876542488 @default.
• W1543294597 cites W1965372133 @default.
• W1543294597 cites W1974815914 @default.
• W1543294597 cites W1975584136 @default.
• W1543294597 cites W1982108913 @default.
• W1543294597 cites W1984525882 @default.
• W1543294597 cites W1993243592 @default.
• W1543294597 cites W1996251228 @default.
• W1543294597 cites W2005606581 @default.
• W1543294597 cites W2007625577 @default.
• W1543294597 cites W2009740988 @default.
• W1543294597 cites W2010601153 @default.
• W1543294597 cites W2015886184 @default.
• W1543294597 cites W2017956702 @default.
• W1543294597 cites W2022687427 @default.
• W1543294597 cites W2026663073 @default.
• W1543294597 cites W2037065330 @default.
• W1543294597 cites W2047217963 @default.
• W1543294597 cites W2050347737 @default.
• W1543294597 cites W2055192078 @default.
• W1543294597 cites W2060913542 @default.
• W1543294597 cites W2072764050 @default.
• W1543294597 cites W2088094960 @default.
• W1543294597 cites W2093339251 @default.
• W1543294597 cites W2109572507 @default.
• W1543294597 cites W2110972790 @default.
• W1543294597 cites W2116815924 @default.
• W1543294597 cites W2118636735 @default.
• W1543294597 cites W2123480703 @default.
• W1543294597 cites W2146310502 @default.
• W1543294597 cites W2155724804 @default.
• W1543294597 cites W2161743101 @default.
• W1543294597 cites W2183043361 @default.
• W1543294597 cites W2221403975 @default.
• W1543294597 cites W4238785539 @default.
• W1543294597 cites W4251890041 @default.
• W1543294597 doi "https://doi.org/10.4049/jimmunol.160.1.241" @default.
• W1543294597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9551977" @default.
• W1543294597 hasPublicationYear "1998" @default.
• W1543294597 type Work @default.
• W1543294597 sameAs 1543294597 @default.
• W1543294597 citedByCount "58" @default.
• W1543294597 countsByYear W15432945972012 @default.
• W1543294597 countsByYear W15432945972013 @default.
• W1543294597 countsByYear W15432945972015 @default.
• W1543294597 countsByYear W15432945972016 @default.
• W1543294597 countsByYear W15432945972017 @default.
• W1543294597 countsByYear W15432945972018 @default.
• W1543294597 countsByYear W15432945972019 @default.
• W1543294597 countsByYear W15432945972020 @default.
• W1543294597 countsByYear W15432945972022 @default.
• W1543294597 crossrefType "journal-article" @default.
• W1543294597 hasAuthorship W1543294597A5040547622 @default.
• W1543294597 hasAuthorship W1543294597A5074237345 @default.
• W1543294597 hasBestOaLocation W15432945971 @default.
• W1543294597 hasConcept C104317684 @default.
• W1543294597 hasConcept C111936080 @default.
• W1543294597 hasConcept C11960822 @default.
• W1543294597 hasConcept C153911025 @default.
• W1543294597 hasConcept C183978625 @default.
• W1543294597 hasConcept C185592680 @default.
• W1543294597 hasConcept C2775880066 @default.
• W1543294597 hasConcept C2776414213 @default.
• W1543294597 hasConcept C501734568 @default.
• W1543294597 hasConcept C552990157 @default.
• W1543294597 hasConcept C55493867 @default.
• W1543294597 hasConcept C86339819 @default.
• W1543294597 hasConcept C86803240 @default.
• W1543294597 hasConcept C95444343 @default.
• W1543294597 hasConceptScore W1543294597C104317684 @default.
• W1543294597 hasConceptScore W1543294597C111936080 @default.
• W1543294597 hasConceptScore W1543294597C11960822 @default.
• W1543294597 hasConceptScore W1543294597C153911025 @default.
• W1543294597 hasConceptScore W1543294597C183978625 @default.
• W1543294597 hasConceptScore W1543294597C185592680 @default.
• W1543294597 hasConceptScore W1543294597C2775880066 @default.
• W1543294597 hasConceptScore W1543294597C2776414213 @default.
• W1543294597 hasConceptScore W1543294597C501734568 @default.
• W1543294597 hasConceptScore W1543294597C552990157 @default.
• W1543294597 hasConceptScore W1543294597C55493867 @default.
• W1543294597 hasConceptScore W1543294597C86339819 @default.
• W1543294597 hasConceptScore W1543294597C86803240 @default.
• W1543294597 hasConceptScore W1543294597C95444343 @default.
• W1543294597 hasIssue "1" @default.
• W1543294597 hasLocation W15432945971 @default.
• W1543294597 hasLocation W15432945972 @default.

• next