Matches in SemOpenAlex for { <https://semopenalex.org/work/W1543451412> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W1543451412 endingPage "43.1" @default.
- W1543451412 startingPage "43.1" @default.
- W1543451412 abstract "Abstract We examined B1 and B2 B-cell repertoires in Cryptococcus neoformans infection in mice. Flow-cytometry based antigen-specific staining for B-cell populations generated in response to pulmonary infection was performed using labeled un-encapsulated and encapsulated C. neoformans strains. FACS sorting and single-cell nested PCR was employed to characterize the IgH repertoire. An increase in total peritoneal B1a, B1b and B2 cell numbers was observed as early as day 3 post-infection. Frequencies of both acapsular and capsular antigen-specific cells were increased in the B1a subpopulation (18%, 5%) in infected compared to naïve mice (11%, 2%). The heavy chains of antigen-specific B2 and B1b cells recognizing acapsular and capsular cryptococcus belonged predominantly to the VH1 family. Interestingly, increased VH11 (22%) and VH12 (33%) usage was observed in antigen-specific B1a cells recognizing acapsular and encapsulated cryptococcus, respectively, compared to the conventional B1a population. Although all acapsular (VH11+) and capsular (VH12+) B1a cells used the same VH11.2.53 and VH12.1.78 genes, 60% and 80% rearrangements were unique, respectively. VH genes in both cases were essentially germline, however 40% of acapsular specific B1a cells had 4 or more N-nucleotide additions compared to capsular specific B1a cells (20%). These results indicate that naturally occurring phosphatidyl choline reactive B1a cells have broad specificities and can bind to encapsulated cryptococcus." @default.
- W1543451412 created "2016-06-24" @default.
- W1543451412 creator A5002812281 @default.
- W1543451412 creator A5064008115 @default.
- W1543451412 date "2012-05-01" @default.
- W1543451412 modified "2023-10-18" @default.
- W1543451412 title "Molecular characterization of antigen-specific B1 and B2 B-cells populations in <i>Cryptococcus neoformans</i> infection in mice (43.1)" @default.
- W1543451412 doi "https://doi.org/10.4049/jimmunol.188.supp.43.1" @default.
- W1543451412 hasPublicationYear "2012" @default.
- W1543451412 type Work @default.
- W1543451412 sameAs 1543451412 @default.
- W1543451412 citedByCount "0" @default.
- W1543451412 crossrefType "journal-article" @default.
- W1543451412 hasAuthorship W1543451412A5002812281 @default.
- W1543451412 hasAuthorship W1543451412A5064008115 @default.
- W1543451412 hasConcept C147483822 @default.
- W1543451412 hasConcept C153911025 @default.
- W1543451412 hasConcept C203014093 @default.
- W1543451412 hasConcept C2777893358 @default.
- W1543451412 hasConcept C2778630268 @default.
- W1543451412 hasConcept C2908647359 @default.
- W1543451412 hasConcept C553184892 @default.
- W1543451412 hasConcept C71924100 @default.
- W1543451412 hasConcept C86803240 @default.
- W1543451412 hasConcept C89423630 @default.
- W1543451412 hasConcept C99454951 @default.
- W1543451412 hasConceptScore W1543451412C147483822 @default.
- W1543451412 hasConceptScore W1543451412C153911025 @default.
- W1543451412 hasConceptScore W1543451412C203014093 @default.
- W1543451412 hasConceptScore W1543451412C2777893358 @default.
- W1543451412 hasConceptScore W1543451412C2778630268 @default.
- W1543451412 hasConceptScore W1543451412C2908647359 @default.
- W1543451412 hasConceptScore W1543451412C553184892 @default.
- W1543451412 hasConceptScore W1543451412C71924100 @default.
- W1543451412 hasConceptScore W1543451412C86803240 @default.
- W1543451412 hasConceptScore W1543451412C89423630 @default.
- W1543451412 hasConceptScore W1543451412C99454951 @default.
- W1543451412 hasIssue "1_Supplement" @default.
- W1543451412 hasLocation W15434514121 @default.
- W1543451412 hasOpenAccess W1543451412 @default.
- W1543451412 hasPrimaryLocation W15434514121 @default.
- W1543451412 hasRelatedWork W1973038690 @default.
- W1543451412 hasRelatedWork W2005800004 @default.
- W1543451412 hasRelatedWork W2102679273 @default.
- W1543451412 hasRelatedWork W2124593326 @default.
- W1543451412 hasRelatedWork W2169420175 @default.
- W1543451412 hasRelatedWork W2322049042 @default.
- W1543451412 hasRelatedWork W2355593916 @default.
- W1543451412 hasRelatedWork W2609326071 @default.
- W1543451412 hasRelatedWork W2912010737 @default.
- W1543451412 hasRelatedWork W3009677009 @default.
- W1543451412 hasVolume "188" @default.
- W1543451412 isParatext "false" @default.
- W1543451412 isRetracted "false" @default.
- W1543451412 magId "1543451412" @default.
- W1543451412 workType "article" @default.