FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1543574734> ?p ?o ?g. }

• W1543574734 endingPage "168" @default.
• W1543574734 startingPage "159" @default.
• W1543574734 abstract "Abstract Importance of microparticles (MPs), also regarded as extracellular vesicles, in many physiological processes and clinical conditions motivates one to use the most informative and precise methods for their characterization. Methods based on individual particle analysis provide statistically reliable distributions of MP population over characteristics. Although flow cytometry is one of the most powerful technologies of this type, the standard forward‐versus‐side‐scattering plots of MPs and platelets (PLTs) overlap considerably because of similarity of their morphological characteristics. Moreover, ordinary flow cytometry is not capable of measurement of size and refractive index (RI) of MPs. In this study, we 1) employed the potential of the scanning flow cytometer (SFC) for identification and characterization of MPs from light scattering; 2) suggested the reference method to characterize MP morphology (size and RI) with high precision; and 3) determined the lowest size of a MP that can be characterized from light scattering with the SFC. We equipped the SFC with 405 and 488 nm lasers to measure the light‐scattering profiles and side scattering from MPs, respectively. The developed two‐stage method allowed accurate separation of PLTs and MPs in platelet‐rich plasma. We used two optical models for MPs, a sphere and a bisphere, in the solution of the inverse light‐scattering problem. This solution provides unprecedented precision in determination of size and RI of individual spherical MPs—median uncertainties (standard deviations) were 6 nm and 0.003, respectively. The developed method provides instrument‐independent quantitative information on MPs, which can be used in studies of various factors affecting MP population. © 2015 International Society for Advancement of Cytometry" @default.
• W1543574734 created "2016-06-24" @default.
• W1543574734 creator A5011186655 @default.
• W1543574734 creator A5012445431 @default.
• W1543574734 creator A5019042579 @default.
• W1543574734 creator A5019144370 @default.
• W1543574734 creator A5037442751 @default.
• W1543574734 creator A5058470812 @default.
• W1543574734 creator A5079938369 @default.
• W1543574734 date "2015-03-21" @default.
• W1543574734 modified "2023-10-16" @default.
• W1543574734 title "Super-resolved calibration-free flow cytometric characterization of platelets and cell-derived microparticles in platelet-rich plasma" @default.
• W1543574734 cites W1493050817 @default.
• W1543574734 cites W1562385120 @default.
• W1543574734 cites W1693284829 @default.
• W1543574734 cites W1880254070 @default.
• W1543574734 cites W1959180550 @default.
• W1543574734 cites W1968118776 @default.
• W1543574734 cites W1974310585 @default.
• W1543574734 cites W1975775601 @default.
• W1543574734 cites W2003909308 @default.
• W1543574734 cites W2019072696 @default.
• W1543574734 cites W2020106202 @default.
• W1543574734 cites W2025740589 @default.
• W1543574734 cites W2031336341 @default.
• W1543574734 cites W2034717642 @default.
• W1543574734 cites W2044638539 @default.
• W1543574734 cites W2049802698 @default.
• W1543574734 cites W2052334426 @default.
• W1543574734 cites W2062155172 @default.
• W1543574734 cites W2071578270 @default.
• W1543574734 cites W2081069642 @default.
• W1543574734 cites W2086275775 @default.
• W1543574734 cites W2088137738 @default.
• W1543574734 cites W2089851362 @default.
• W1543574734 cites W2089943045 @default.
• W1543574734 cites W2100927341 @default.
• W1543574734 cites W2102666544 @default.
• W1543574734 cites W2112995502 @default.
• W1543574734 cites W2113741278 @default.
• W1543574734 cites W2114495365 @default.
• W1543574734 cites W2117890823 @default.
• W1543574734 cites W2122409409 @default.
• W1543574734 cites W2123030744 @default.
• W1543574734 cites W2124242076 @default.
• W1543574734 cites W2125087964 @default.
• W1543574734 cites W2125542949 @default.
• W1543574734 cites W2137958295 @default.
• W1543574734 cites W2139406636 @default.
• W1543574734 cites W2140527652 @default.
• W1543574734 cites W2142512073 @default.
• W1543574734 cites W2146920208 @default.
• W1543574734 cites W2157324002 @default.
• W1543574734 cites W2168175751 @default.
• W1543574734 cites W2335196959 @default.
• W1543574734 cites W2476651486 @default.
• W1543574734 cites W4243563432 @default.
• W1543574734 cites W77107658 @default.
• W1543574734 doi "https://doi.org/10.1002/cyto.a.22621" @default.
• W1543574734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25808430" @default.
• W1543574734 hasPublicationYear "2015" @default.
• W1543574734 type Work @default.
• W1543574734 sameAs 1543574734 @default.
• W1543574734 citedByCount "27" @default.
• W1543574734 countsByYear W15435747342015 @default.
• W1543574734 countsByYear W15435747342016 @default.
• W1543574734 countsByYear W15435747342017 @default.
• W1543574734 countsByYear W15435747342018 @default.
• W1543574734 countsByYear W15435747342019 @default.
• W1543574734 countsByYear W15435747342020 @default.
• W1543574734 countsByYear W15435747342021 @default.
• W1543574734 countsByYear W15435747342022 @default.
• W1543574734 countsByYear W15435747342023 @default.
• W1543574734 crossrefType "journal-article" @default.
• W1543574734 hasAuthorship W1543574734A5011186655 @default.
• W1543574734 hasAuthorship W1543574734A5012445431 @default.
• W1543574734 hasAuthorship W1543574734A5019042579 @default.
• W1543574734 hasAuthorship W1543574734A5019144370 @default.
• W1543574734 hasAuthorship W1543574734A5037442751 @default.
• W1543574734 hasAuthorship W1543574734A5058470812 @default.
• W1543574734 hasAuthorship W1543574734A5079938369 @default.
• W1543574734 hasConcept C105795698 @default.
• W1543574734 hasConcept C120456961 @default.
• W1543574734 hasConcept C120665830 @default.
• W1543574734 hasConcept C121332964 @default.
• W1543574734 hasConcept C144024400 @default.
• W1543574734 hasConcept C149923435 @default.
• W1543574734 hasConcept C165838908 @default.
• W1543574734 hasConcept C185592680 @default.
• W1543574734 hasConcept C186060115 @default.
• W1543574734 hasConcept C191486275 @default.
• W1543574734 hasConcept C192562407 @default.
• W1543574734 hasConcept C203014093 @default.
• W1543574734 hasConcept C2780339063 @default.
• W1543574734 hasConcept C2780841128 @default.
• W1543574734 hasConcept C2908647359 @default.
• W1543574734 hasConcept C33923547 @default.
• W1543574734 hasConcept C54355233 @default.

• next