Matches in SemOpenAlex for { <https://semopenalex.org/work/W1543636223> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W1543636223 endingPage "10" @default.
- W1543636223 startingPage "1" @default.
- W1543636223 abstract "The nucleocytoplasmic hybrid combination performed by nuclear graft between B. bufo nucleus and B. calamita egg is 95% lethal during gastrulation mostly at the young gastrula stage (75%) and to a lesser extent at the yolk plug stage (20%). The heterologous B. calamita cytoplasm was treated or fractioned by different techniques including centrifugation, nucleic acid extraction, ammonium sulfate treatment and heating. The treated cytoplasmic fractions were injected in B. bufo fertilized eggs in order to check their influence on morphogenetic capacities of the B. bufo nucleus. Injection of hyaline cytoplasmic fraction obtained from B. calamita centrifuged eggs blocks the B. Bufo fertilized egg gastrulation in similar rates as in nucleocytoplasmic association performed by interspecific nuclear grafting. Gastrulation, however, is not affected by injection of total nucleic acids extracted from this hyaline fraction. The soluble cytoplasmic fraction obtained after 60% ammonium sulfate precipitation blocks the development at the beginning of gastrulation, this property being inactivated by heating for 5 min at 60°C. Supernatant from 70% ammonium sulfate precipitation blocks the development at the yolk plug stage and is inactivated by heating for 5 min at 80°C. These experiments suggest that two distinct factors in B. calamita eggs are involved in developmental arrest of injected B. bufo fertilized eggs, these two factors respectively acting on the beginning and on the end of gastrulation." @default.
- W1543636223 created "2016-06-24" @default.
- W1543636223 creator A5070832975 @default.
- W1543636223 creator A5078085008 @default.
- W1543636223 date "1984-02-01" @default.
- W1543636223 modified "2023-09-27" @default.
- W1543636223 title "Implication of Cytoplasmic Factors in the Lethality of Bufonidae Nucleocytoplasmic Hybrids. (cytoplasmic factors/nucleocytoplasmic hybrids/bufonidae)" @default.
- W1543636223 cites W1973433627 @default.
- W1543636223 cites W1985757571 @default.
- W1543636223 cites W1989702632 @default.
- W1543636223 cites W1991276711 @default.
- W1543636223 cites W1997998593 @default.
- W1543636223 cites W2015517539 @default.
- W1543636223 cites W2018074127 @default.
- W1543636223 cites W2029248285 @default.
- W1543636223 cites W2035330819 @default.
- W1543636223 cites W2043778312 @default.
- W1543636223 cites W2053195914 @default.
- W1543636223 cites W2063917737 @default.
- W1543636223 cites W2066384411 @default.
- W1543636223 cites W2071707765 @default.
- W1543636223 cites W2085884505 @default.
- W1543636223 cites W2107332774 @default.
- W1543636223 cites W2130805712 @default.
- W1543636223 cites W2415125036 @default.
- W1543636223 doi "https://doi.org/10.1111/j.1440-169x.1984.00001.x" @default.
- W1543636223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37282041" @default.
- W1543636223 hasPublicationYear "1984" @default.
- W1543636223 type Work @default.
- W1543636223 sameAs 1543636223 @default.
- W1543636223 citedByCount "2" @default.
- W1543636223 crossrefType "journal-article" @default.
- W1543636223 hasAuthorship W1543636223A5070832975 @default.
- W1543636223 hasAuthorship W1543636223A5078085008 @default.
- W1543636223 hasConcept C129536746 @default.
- W1543636223 hasConcept C153911025 @default.
- W1543636223 hasConcept C18903297 @default.
- W1543636223 hasConcept C190062978 @default.
- W1543636223 hasConcept C196843134 @default.
- W1543636223 hasConcept C2777836633 @default.
- W1543636223 hasConcept C2778166480 @default.
- W1543636223 hasConcept C2780723820 @default.
- W1543636223 hasConcept C2781321622 @default.
- W1543636223 hasConcept C59822182 @default.
- W1543636223 hasConcept C86803240 @default.
- W1543636223 hasConcept C87073359 @default.
- W1543636223 hasConcept C95444343 @default.
- W1543636223 hasConceptScore W1543636223C129536746 @default.
- W1543636223 hasConceptScore W1543636223C153911025 @default.
- W1543636223 hasConceptScore W1543636223C18903297 @default.
- W1543636223 hasConceptScore W1543636223C190062978 @default.
- W1543636223 hasConceptScore W1543636223C196843134 @default.
- W1543636223 hasConceptScore W1543636223C2777836633 @default.
- W1543636223 hasConceptScore W1543636223C2778166480 @default.
- W1543636223 hasConceptScore W1543636223C2780723820 @default.
- W1543636223 hasConceptScore W1543636223C2781321622 @default.
- W1543636223 hasConceptScore W1543636223C59822182 @default.
- W1543636223 hasConceptScore W1543636223C86803240 @default.
- W1543636223 hasConceptScore W1543636223C87073359 @default.
- W1543636223 hasConceptScore W1543636223C95444343 @default.
- W1543636223 hasIssue "1" @default.
- W1543636223 hasLocation W15436362231 @default.
- W1543636223 hasLocation W15436362232 @default.
- W1543636223 hasOpenAccess W1543636223 @default.
- W1543636223 hasPrimaryLocation W15436362231 @default.
- W1543636223 hasRelatedWork W2054487448 @default.
- W1543636223 hasRelatedWork W2092123140 @default.
- W1543636223 hasRelatedWork W2108915127 @default.
- W1543636223 hasRelatedWork W2111354472 @default.
- W1543636223 hasRelatedWork W2169340257 @default.
- W1543636223 hasRelatedWork W2182072250 @default.
- W1543636223 hasRelatedWork W2185042658 @default.
- W1543636223 hasRelatedWork W2398315340 @default.
- W1543636223 hasRelatedWork W2988958840 @default.
- W1543636223 hasRelatedWork W3178209818 @default.
- W1543636223 hasVolume "26" @default.
- W1543636223 isParatext "false" @default.
- W1543636223 isRetracted "false" @default.
- W1543636223 magId "1543636223" @default.
- W1543636223 workType "article" @default.