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- W1543749345 abstract "Previously we expressed rat 25‐hydroxyvitamin D 3 24‐hydroxylase (CYP24) cDNA in Escherichia coli JM109 and showed that CYP24 catalyses three‐step monooxygenation towards 25‐hydroxyvitamin D 3 and 1α,25‐dihydroxyvitamin D 3 [Akiyoshi‐Shibata, M., Sakaki, T., Ohyama, Y., Noshiro, M., Okuda, K. & Yabusaki, Y. (1994) Eur. J. Biochem . 224 , 335–343]. In this study, we demonstrate further oxidation by CYP24 including four‐ and six‐step monooxygenation towards 25‐hydroxyvitamin D 3 and 1α,25‐dihydroxyvitamin D 3 , respectively. When the substrate 25‐hydroxyvitamin D 3 was added to a culture of recombinant E. coli , four metabolites, 24,25‐dihydroxyvitamin D 3 , 24‐oxo‐25‐hydroxyvitamin D 3 , 24‐oxo‐23,25‐dihydroxyvitamin D 3 and 24,25,26,27‐tetranor‐23‐hydroxyvitamin D 3 were observed. These results indicate that CYP24 catalyses at least four‐step monooxygenation toward 25‐hydroxyvitamin D 3 . Furthermore, in‐vivo and in‐vitro metabolic studies on 1α,25‐dihydroxyvitamin D 3 clearly indicated that CYP24 catalyses six‐step monooxygenation to convert 1α,25‐dihydroxyvitamin D 3 into calcitroic acid which is known as a final metabolite of 1α,25‐dihydroxyvitamin D 3 for excretion in bile. These results strongly suggest that CYP24 is largely responsible for the metabolism of both 25‐hydroxyvitamin D 3 and 1α,25‐dihydroxyvitamin D 3 ." @default.
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- W1543749345 date "1999-05-15" @default.
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- W1543749345 title "Metabolic studies using recombinant Escherichia coli cells producing rat mitochondrial CYP24 . CYP24 can convert 1alpha,25-dihydroxyvitamin D3 to calcitroic acid" @default.
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- W1543749345 doi "https://doi.org/10.1046/j.1432-1327.1999.00375.x" @default.
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