Matches in SemOpenAlex for { <https://semopenalex.org/work/W1544386985> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W1544386985 abstract "G protein-coupled receptor kinase 5 (GRK5) is one of the seven GRK family members whose primary function is to desensitize G protein-coupled receptors (GPCRs). In recently, GRK5 deficiency has been linked to early Alzheimer disease (AD), but the mechanism by which GRK5 deficiency may accelerate to AD pathogenesis remains elusive. The GRK5 mRNA is expressed widely in brain and peripheral tissues, with highest expression evident heart, lung, and placenta. In cellular model systems, GRK5 can phosphorylate several neuronal GPCRs including ß2 adrenergic, M2-muscarinic, secretin, angiotensin AT1, and thyroid stimulating hormone receptors. Transcription activator-like effector nucleases (TALENs) are programmable nucleases that join FokI endonucleases with the modular DNA-binding domain of TALEs and highly effective in inducing mutations at specific genome loci. TALEN-mediated mutagenesis in zygotes is a potential alternative to conventional gene targeting in mice. In the presented study, we report the generation of mice with genetic knockout of the GRK gene using TALENs. We designed TALEN vectors for exon 1, 3 and 5 of mouse GRK5 gene and tested their ability to alter the each surrogate vector in 293T cells. We prepared of mRNAs for the linearized TALEN using the mMessage mMachine T7 Ultra kit. mRNAs (4ng/μl) was injected into cytoplasm of 180 one-cell embryos. After incubation for 24 hours, the selected two-cell embryos transferred into the oviduct of seven pseudopregnant C57BL/6 mice. We confirmed the genotype of Fo mice by sequencing and T7E1 assay. Grant Funding Source: Supported by BK 21 plus program, the Ministry of Education, Science and Technology, Korea" @default.
- W1544386985 created "2016-06-24" @default.
- W1544386985 creator A5008362503 @default.
- W1544386985 creator A5015407605 @default.
- W1544386985 creator A5018742534 @default.
- W1544386985 creator A5019124360 @default.
- W1544386985 creator A5089513813 @default.
- W1544386985 date "2014-04-01" @default.
- W1544386985 modified "2023-09-22" @default.
- W1544386985 title "GRK5 knockout mice created by TALEN‐mediated gene targeting (576.2)" @default.
- W1544386985 doi "https://doi.org/10.1096/fasebj.28.1_supplement.576.2" @default.
- W1544386985 hasPublicationYear "2014" @default.
- W1544386985 type Work @default.
- W1544386985 sameAs 1544386985 @default.
- W1544386985 citedByCount "0" @default.
- W1544386985 crossrefType "journal-article" @default.
- W1544386985 hasAuthorship W1544386985A5008362503 @default.
- W1544386985 hasAuthorship W1544386985A5015407605 @default.
- W1544386985 hasAuthorship W1544386985A5018742534 @default.
- W1544386985 hasAuthorship W1544386985A5019124360 @default.
- W1544386985 hasAuthorship W1544386985A5089513813 @default.
- W1544386985 hasConcept C104317684 @default.
- W1544386985 hasConcept C111829913 @default.
- W1544386985 hasConcept C144501496 @default.
- W1544386985 hasConcept C153911025 @default.
- W1544386985 hasConcept C31909778 @default.
- W1544386985 hasConcept C54355233 @default.
- W1544386985 hasConcept C77957584 @default.
- W1544386985 hasConcept C86803240 @default.
- W1544386985 hasConcept C95444343 @default.
- W1544386985 hasConcept C98108389 @default.
- W1544386985 hasConceptScore W1544386985C104317684 @default.
- W1544386985 hasConceptScore W1544386985C111829913 @default.
- W1544386985 hasConceptScore W1544386985C144501496 @default.
- W1544386985 hasConceptScore W1544386985C153911025 @default.
- W1544386985 hasConceptScore W1544386985C31909778 @default.
- W1544386985 hasConceptScore W1544386985C54355233 @default.
- W1544386985 hasConceptScore W1544386985C77957584 @default.
- W1544386985 hasConceptScore W1544386985C86803240 @default.
- W1544386985 hasConceptScore W1544386985C95444343 @default.
- W1544386985 hasConceptScore W1544386985C98108389 @default.
- W1544386985 hasIssue "S1" @default.
- W1544386985 hasLocation W15443869851 @default.
- W1544386985 hasOpenAccess W1544386985 @default.
- W1544386985 hasPrimaryLocation W15443869851 @default.
- W1544386985 hasRelatedWork W1992096920 @default.
- W1544386985 hasRelatedWork W2008039196 @default.
- W1544386985 hasRelatedWork W2052982315 @default.
- W1544386985 hasRelatedWork W2059149167 @default.
- W1544386985 hasRelatedWork W2072816026 @default.
- W1544386985 hasRelatedWork W2073000562 @default.
- W1544386985 hasRelatedWork W2144178569 @default.
- W1544386985 hasRelatedWork W2146662935 @default.
- W1544386985 hasRelatedWork W2372139093 @default.
- W1544386985 hasRelatedWork W2604016375 @default.
- W1544386985 hasVolume "28" @default.
- W1544386985 isParatext "false" @default.
- W1544386985 isRetracted "false" @default.
- W1544386985 magId "1544386985" @default.
- W1544386985 workType "article" @default.