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- W1544415700 abstract "Abstract The metabolism of [3H]estrone sulfate as well as the intranuclear binding of estrone sulfate and its metabolites, estrone and estradiol, was studied in tissue minces of anterior pituitary, hypothalamus, and cerebral cortex of female sheep. Estrone sulfate was converted to unconjugated estrogens to approximately the same degree in all three tissues. Nuclei were prepared by sucrose density gradient plus Triton X-100 treatment. Following incubation of tissue minces with [3H]estrone sulfate, no [3H]estrone sulfate was found associated with nuclei. Only in anterior pituitary was specific intranuclear association of estrone or estradiol demonstrated. Ovariectomy resulted in large increases in the intranuclear association of both these estrogens in anterior pituitary. On the basis of 0.6 m KCl extraction of purified nuclei from the three tissues and subjection of the extracts to gel filtration on Sephadex G-25, steroid-macromolecular complexes could be demonstrated only in anterior pituitary. Analysis of these complexes indicated that 70% of the radioactivity associated with the nuclear macromolecular receptor complexes was estradiol and 30% estrone." @default.
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- W1544415700 date "1973-03-01" @default.
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- W1544415700 title "Intranuclear Binding of 17β-Estradiol and Estrone in Female Ovine Pituitaries following Incubation with Estrone Sulfate" @default.
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- W1544415700 doi "https://doi.org/10.1016/s0021-9258(19)44232-4" @default.
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