Matches in SemOpenAlex for { <https://semopenalex.org/work/W1544790717> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1544790717 endingPage "303" @default.
- W1544790717 startingPage "286" @default.
- W1544790717 abstract "Patterned antigen-binding cells (ABC) can bind two antigens and show “islands” of Ig receptor with mixed specificity. However, these cells, when unfixed, lose most of their bound fluorescent antigen within minutes upon warming above 0 °C. Residual antigen moves to one pole of the cell forming a “cap” within 5 min at room temperature. If such patterned ABC are capped with a single antigen, receptors to a second antigen can be detected on a portion of the capped cells, but only in the cap. The frequency of capped, “double” ABC approximated the frequency of patterned “double” ABC originally present. If lymphoid cells are mixed with fluorescent antigens at 0 °C and then incubated for 4 hr at 37 °, no ABC are found. When the cells are then fixed and the fluorescent antigens readded, new antigen-binding Ig receptors can be shown to have reappeared on the cell surface during the 4-hr incubation. The reappearance of antigen receptor could be inhibited by prior addition of either 10−2M sodium azide or 50 μg/ml cycloheximide, implying that the receptors were actively synthesized by the cell. These inhibitors did not prevent shedding, but azide did inhibit the capping process. Both B-cells (bone marrow or spleen cells) and T-cells (splenic T-cells or 99.5% pure cortisone-resistant T-cells) were shown to regenerate multispecific ABC to the frequency found prior to incubation." @default.
- W1544790717 created "2016-06-24" @default.
- W1544790717 creator A5077415247 @default.
- W1544790717 creator A5081362680 @default.
- W1544790717 creator A5083903886 @default.
- W1544790717 date "1975-08-01" @default.
- W1544790717 modified "2023-09-26" @default.
- W1544790717 title "Antigen binding to lymphoid cells from unimmunized mice" @default.
- W1544790717 cites W124127999 @default.
- W1544790717 cites W1482401694 @default.
- W1544790717 cites W1499774836 @default.
- W1544790717 cites W1544790717 @default.
- W1544790717 cites W1589389703 @default.
- W1544790717 cites W1663364610 @default.
- W1544790717 cites W1995111076 @default.
- W1544790717 cites W2013295182 @default.
- W1544790717 cites W2029194296 @default.
- W1544790717 cites W2035151793 @default.
- W1544790717 cites W2047363885 @default.
- W1544790717 cites W2059653141 @default.
- W1544790717 cites W2063999184 @default.
- W1544790717 cites W2069310769 @default.
- W1544790717 cites W2079757266 @default.
- W1544790717 cites W2126745783 @default.
- W1544790717 cites W2169102863 @default.
- W1544790717 cites W2171804984 @default.
- W1544790717 doi "https://doi.org/10.1016/0008-8749(75)90058-1" @default.
- W1544790717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1095215" @default.
- W1544790717 hasPublicationYear "1975" @default.
- W1544790717 type Work @default.
- W1544790717 sameAs 1544790717 @default.
- W1544790717 citedByCount "19" @default.
- W1544790717 crossrefType "journal-article" @default.
- W1544790717 hasAuthorship W1544790717A5077415247 @default.
- W1544790717 hasAuthorship W1544790717A5081362680 @default.
- W1544790717 hasAuthorship W1544790717A5083903886 @default.
- W1544790717 hasConcept C147483822 @default.
- W1544790717 hasConcept C1491633281 @default.
- W1544790717 hasConcept C153911025 @default.
- W1544790717 hasConcept C159654299 @default.
- W1544790717 hasConcept C170493617 @default.
- W1544790717 hasConcept C203014093 @default.
- W1544790717 hasConcept C25642318 @default.
- W1544790717 hasConcept C2778128677 @default.
- W1544790717 hasConcept C2780931953 @default.
- W1544790717 hasConcept C2781417787 @default.
- W1544790717 hasConcept C3675279 @default.
- W1544790717 hasConcept C55493867 @default.
- W1544790717 hasConcept C86803240 @default.
- W1544790717 hasConceptScore W1544790717C147483822 @default.
- W1544790717 hasConceptScore W1544790717C1491633281 @default.
- W1544790717 hasConceptScore W1544790717C153911025 @default.
- W1544790717 hasConceptScore W1544790717C159654299 @default.
- W1544790717 hasConceptScore W1544790717C170493617 @default.
- W1544790717 hasConceptScore W1544790717C203014093 @default.
- W1544790717 hasConceptScore W1544790717C25642318 @default.
- W1544790717 hasConceptScore W1544790717C2778128677 @default.
- W1544790717 hasConceptScore W1544790717C2780931953 @default.
- W1544790717 hasConceptScore W1544790717C2781417787 @default.
- W1544790717 hasConceptScore W1544790717C3675279 @default.
- W1544790717 hasConceptScore W1544790717C55493867 @default.
- W1544790717 hasConceptScore W1544790717C86803240 @default.
- W1544790717 hasIssue "2" @default.
- W1544790717 hasLocation W15447907171 @default.
- W1544790717 hasLocation W15447907172 @default.
- W1544790717 hasOpenAccess W1544790717 @default.
- W1544790717 hasPrimaryLocation W15447907171 @default.
- W1544790717 hasRelatedWork W1973484362 @default.
- W1544790717 hasRelatedWork W1975188916 @default.
- W1544790717 hasRelatedWork W1975839908 @default.
- W1544790717 hasRelatedWork W1980119493 @default.
- W1544790717 hasRelatedWork W2087539308 @default.
- W1544790717 hasRelatedWork W2136688122 @default.
- W1544790717 hasRelatedWork W2256645954 @default.
- W1544790717 hasRelatedWork W2321160190 @default.
- W1544790717 hasRelatedWork W2402858051 @default.
- W1544790717 hasRelatedWork W2944861833 @default.
- W1544790717 hasVolume "18" @default.
- W1544790717 isParatext "false" @default.
- W1544790717 isRetracted "false" @default.
- W1544790717 magId "1544790717" @default.
- W1544790717 workType "article" @default.