FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1544947105> ?p ?o ?g. }

• W1544947105 abstract "We have successfully developed a nucleic acid extraction system based on a microacoustic lysis array coupled to an integrated nucleic acid extraction system all on a single cartridge. The microacoustic lysing array is based on 36{sup o} Y cut lithium niobate, which couples bulk acoustic waves (BAW) into the microchannels. The microchannels were fabricated using Mylar laminates and fused silica to form acoustic-fluidic interface cartridges. The transducer array consists of four active elements directed for cell lysis and one optional BAW element for mixing on the cartridge. The lysis system was modeled using one dimensional (1D) transmission line and two dimensional (2D) FEM models. For input powers required to lyse cells, the flow rate dictated the temperature change across the lysing region. From the computational models, a flow rate of 10 {micro}L/min produced a temperature rise of 23.2 C and only 6.7 C when flowing at 60 {micro}L/min. The measured temperature changes were 5 C less than the model. The computational models also permitted optimization of the acoustic coupling to the microchannel region and revealed the potential impact of thermal effects if not controlled. Using E. coli, we achieved a lysing efficacy of 49.9 {+-} 29.92 % based on amore » cell viability assay with a 757.2 % increase in ATP release within 20 seconds of acoustic exposure. A bench-top lysing system required 15-20 minutes operating up to 58 Watts to achieve the same level of cell lysis. We demonstrate that active mixing on the cartridge was critical to maximize binding and release of nucleic acid to the magnetic beads. Using a sol-gel silica bead matrix filled microchannel the extraction efficacy was 40%. The cartridge based magnetic bead system had an extraction efficiency of 19.2%. For an electric field based method that used Nafion films, a nucleic acid extraction efficiency of 66.3 % was achieved at 6 volts DC. For the flow rates we tested (10-50 {micro}L/min), the nucleic acid extraction time was 5-10 minutes for a volume of 50 {micro}L. Moreover, a unique feature of this technology is the ability to replace the cartridges for subsequent nucleic acid extractions.« less" @default.
• W1544947105 created "2016-06-24" @default.
• W1544947105 creator A5016516903 @default.
• W1544947105 creator A5017356564 @default.
• W1544947105 creator A5030734212 @default.
• W1544947105 creator A5035616320 @default.
• W1544947105 creator A5044929617 @default.
• W1544947105 creator A5063028481 @default.
• W1544947105 creator A5064685466 @default.
• W1544947105 creator A5071658484 @default.
• W1544947105 creator A5073430053 @default.
• W1544947105 creator A5080457574 @default.
• W1544947105 creator A5081129732 @default.
• W1544947105 date "2009-09-01" @default.
• W1544947105 modified "2023-09-24" @default.
• W1544947105 title "Intelligent front-end sample preparation tool using acoustic streaming." @default.
• W1544947105 cites W1588533534 @default.
• W1544947105 cites W1652103818 @default.
• W1544947105 cites W1998095407 @default.
• W1544947105 cites W1999407915 @default.
• W1544947105 cites W2000683678 @default.
• W1544947105 cites W2003278970 @default.
• W1544947105 cites W2021627394 @default.
• W1544947105 cites W2040979877 @default.
• W1544947105 cites W2067912898 @default.
• W1544947105 cites W2083767189 @default.
• W1544947105 cites W2107246074 @default.
• W1544947105 cites W2150704355 @default.
• W1544947105 cites W2157532773 @default.
• W1544947105 cites W2159794613 @default.
• W1544947105 cites W2163550415 @default.
• W1544947105 cites W2166229810 @default.
• W1544947105 cites W2468878123 @default.
• W1544947105 doi "https://doi.org/10.2172/993903" @default.
• W1544947105 hasPublicationYear "2009" @default.
• W1544947105 type Work @default.
• W1544947105 sameAs 1544947105 @default.
• W1544947105 citedByCount "0" @default.
• W1544947105 crossrefType "report" @default.
• W1544947105 hasAuthorship W1544947105A5016516903 @default.
• W1544947105 hasAuthorship W1544947105A5017356564 @default.
• W1544947105 hasAuthorship W1544947105A5030734212 @default.
• W1544947105 hasAuthorship W1544947105A5035616320 @default.
• W1544947105 hasAuthorship W1544947105A5044929617 @default.
• W1544947105 hasAuthorship W1544947105A5063028481 @default.
• W1544947105 hasAuthorship W1544947105A5064685466 @default.
• W1544947105 hasAuthorship W1544947105A5071658484 @default.
• W1544947105 hasAuthorship W1544947105A5073430053 @default.
• W1544947105 hasAuthorship W1544947105A5080457574 @default.
• W1544947105 hasAuthorship W1544947105A5081129732 @default.
• W1544947105 hasBestOaLocation W15449471052 @default.
• W1544947105 hasConcept C111919701 @default.
• W1544947105 hasConcept C121332964 @default.
• W1544947105 hasConcept C127413603 @default.
• W1544947105 hasConcept C185592680 @default.
• W1544947105 hasConcept C198531522 @default.
• W1544947105 hasConcept C24890656 @default.
• W1544947105 hasConcept C2777551076 @default.
• W1544947105 hasConcept C2779019082 @default.
• W1544947105 hasConcept C41008148 @default.
• W1544947105 hasConcept C43617362 @default.
• W1544947105 hasConcept C53016008 @default.
• W1544947105 hasConcept C78519656 @default.
• W1544947105 hasConcept C81288441 @default.
• W1544947105 hasConceptScore W1544947105C111919701 @default.
• W1544947105 hasConceptScore W1544947105C121332964 @default.
• W1544947105 hasConceptScore W1544947105C127413603 @default.
• W1544947105 hasConceptScore W1544947105C185592680 @default.
• W1544947105 hasConceptScore W1544947105C198531522 @default.
• W1544947105 hasConceptScore W1544947105C24890656 @default.
• W1544947105 hasConceptScore W1544947105C2777551076 @default.
• W1544947105 hasConceptScore W1544947105C2779019082 @default.
• W1544947105 hasConceptScore W1544947105C41008148 @default.
• W1544947105 hasConceptScore W1544947105C43617362 @default.
• W1544947105 hasConceptScore W1544947105C53016008 @default.
• W1544947105 hasConceptScore W1544947105C78519656 @default.
• W1544947105 hasConceptScore W1544947105C81288441 @default.
• W1544947105 hasLocation W15449471051 @default.
• W1544947105 hasLocation W15449471052 @default.
• W1544947105 hasLocation W15449471053 @default.
• W1544947105 hasOpenAccess W1544947105 @default.
• W1544947105 hasPrimaryLocation W15449471051 @default.
• W1544947105 hasRelatedWork W1992246567 @default.
• W1544947105 hasRelatedWork W2045775227 @default.
• W1544947105 hasRelatedWork W2364010155 @default.
• W1544947105 hasRelatedWork W2365342065 @default.
• W1544947105 hasRelatedWork W3109484932 @default.
• W1544947105 hasRelatedWork W3153291440 @default.
• W1544947105 hasRelatedWork W3158173382 @default.
• W1544947105 hasRelatedWork W571985673 @default.
• W1544947105 hasRelatedWork W622130946 @default.
• W1544947105 hasRelatedWork W1602178951 @default.
• W1544947105 isParatext "false" @default.
• W1544947105 isRetracted "false" @default.
• W1544947105 magId "1544947105" @default.
• W1544947105 workType "report" @default.