FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1545076822> ?p ?o ?g. }

• W1545076822 endingPage "3603" @default.
• W1545076822 startingPage "3603" @default.
• W1545076822 abstract "To investigate the potential effects of chelidonine, the main alkaloid of Chelidonium majus, on telomerase activity and its regulation in HepG2 cells.Cytotoxicity of chelidonine for HepG2 cells was determined by neutral red assay. A modified polymerase chain reaction (PCR)-based telomerase repeat amplification protocol was used to estimate relative telomerase activity in chelidonine-treated cells in comparison with the untreated control cells. Relative expression level of the catalytic subunit of telomerase (hTERT) gene and P-glycoprotein (pgp) were estimated using semi-quantitative real-time reverse transcription-PCR (RT-PCR). Cell senescence in treated cells was demonstrated using a beta-galactosidase test.Cytotoxicity of chelidonine in HepG2 cells was not dose-dependent and tended to reach plateau immediately after the living cells were reduced in number to slightly higher than 50%. However, 12 micromol/L concentration of chelidonine was considered as LD(50), where the maximal attainable effects were realized. Real-time RT-PCR data showed that the expression of pgp increased three-fold in chelidonine treated HepG2 cells in comparison with the untreated controls. Morphologically, treated HepG2 cells showed apoptotic features after 24 h and a small fraction of cells appeared with single blister cell death. The relative expression level of Bcl-2 dropped to less than 50% of control cells at a sub-apoptotic concentration of chelidonine and subsequently increased to higher than 120% at LD(50). Telomerase activity was reduced considerably after administration of very low doses of chelidonine, whereas higher concentrations of chelidonine did not remarkably enhance the effect. Real-time RT-PCR experiments indicated a drastic decrease in expression level of hTERT subunit of telomerase under treatment with chelidonine. Repeated treatment of cells with very low doses of chelidonine caused a decline in growth rate by 4 wk and many of the cells appeared to be aged with large volume and dark staining in the beta-galactosidase assay.Chelidonine reduces telomerase activity through down-regulation of hTERT expression. Senescence induction might not be directly caused by reducing telomerase activity as it occurs after a few population doublings." @default.
• W1545076822 created "2016-06-24" @default.
• W1545076822 creator A5034011528 @default.
• W1545076822 creator A5066578159 @default.
• W1545076822 date "2009-01-01" @default.
• W1545076822 modified "2023-09-30" @default.
• W1545076822 title "Transcriptional down regulation of hTERT and senescence induction in HepG2 cells by chelidonine" @default.
• W1545076822 cites W1496772673 @default.
• W1545076822 cites W1496886519 @default.
• W1545076822 cites W1959324886 @default.
• W1545076822 cites W1965695542 @default.
• W1545076822 cites W1971819079 @default.
• W1545076822 cites W1977324053 @default.
• W1545076822 cites W1998792806 @default.
• W1545076822 cites W2008568206 @default.
• W1545076822 cites W2010955901 @default.
• W1545076822 cites W2014519057 @default.
• W1545076822 cites W2017752436 @default.
• W1545076822 cites W2036478856 @default.
• W1545076822 cites W2042371945 @default.
• W1545076822 cites W2042377388 @default.
• W1545076822 cites W2052080135 @default.
• W1545076822 cites W2058114042 @default.
• W1545076822 cites W2062776530 @default.
• W1545076822 cites W2081821799 @default.
• W1545076822 cites W2086200562 @default.
• W1545076822 cites W2086654944 @default.
• W1545076822 cites W2093529099 @default.
• W1545076822 cites W2104908670 @default.
• W1545076822 cites W2141381598 @default.
• W1545076822 cites W2145344158 @default.
• W1545076822 cites W2147319330 @default.
• W1545076822 cites W2155043438 @default.
• W1545076822 cites W2164023792 @default.
• W1545076822 cites W2315523562 @default.
• W1545076822 cites W4293247451 @default.
• W1545076822 doi "https://doi.org/10.3748/wjg.15.3603" @default.
• W1545076822 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2721233" @default.
• W1545076822 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19653337" @default.
• W1545076822 hasPublicationYear "2009" @default.
• W1545076822 type Work @default.
• W1545076822 sameAs 1545076822 @default.
• W1545076822 citedByCount "42" @default.
• W1545076822 countsByYear W15450768222012 @default.
• W1545076822 countsByYear W15450768222013 @default.
• W1545076822 countsByYear W15450768222014 @default.
• W1545076822 countsByYear W15450768222015 @default.
• W1545076822 countsByYear W15450768222016 @default.
• W1545076822 countsByYear W15450768222017 @default.
• W1545076822 countsByYear W15450768222018 @default.
• W1545076822 countsByYear W15450768222019 @default.
• W1545076822 countsByYear W15450768222020 @default.
• W1545076822 countsByYear W15450768222021 @default.
• W1545076822 countsByYear W15450768222022 @default.
• W1545076822 crossrefType "journal-article" @default.
• W1545076822 hasAuthorship W1545076822A5034011528 @default.
• W1545076822 hasAuthorship W1545076822A5066578159 @default.
• W1545076822 hasBestOaLocation W15450768221 @default.
• W1545076822 hasConcept C104317684 @default.
• W1545076822 hasConcept C109316439 @default.
• W1545076822 hasConcept C125593758 @default.
• W1545076822 hasConcept C150194340 @default.
• W1545076822 hasConcept C153911025 @default.
• W1545076822 hasConcept C190283241 @default.
• W1545076822 hasConcept C202751555 @default.
• W1545076822 hasConcept C4692481 @default.
• W1545076822 hasConcept C55493867 @default.
• W1545076822 hasConcept C86803240 @default.
• W1545076822 hasConcept C94581717 @default.
• W1545076822 hasConceptScore W1545076822C104317684 @default.
• W1545076822 hasConceptScore W1545076822C109316439 @default.
• W1545076822 hasConceptScore W1545076822C125593758 @default.
• W1545076822 hasConceptScore W1545076822C150194340 @default.
• W1545076822 hasConceptScore W1545076822C153911025 @default.
• W1545076822 hasConceptScore W1545076822C190283241 @default.
• W1545076822 hasConceptScore W1545076822C202751555 @default.
• W1545076822 hasConceptScore W1545076822C4692481 @default.
• W1545076822 hasConceptScore W1545076822C55493867 @default.
• W1545076822 hasConceptScore W1545076822C86803240 @default.
• W1545076822 hasConceptScore W1545076822C94581717 @default.
• W1545076822 hasIssue "29" @default.
• W1545076822 hasLocation W15450768221 @default.
• W1545076822 hasLocation W15450768222 @default.
• W1545076822 hasLocation W15450768223 @default.
• W1545076822 hasLocation W15450768224 @default.
• W1545076822 hasOpenAccess W1545076822 @default.
• W1545076822 hasPrimaryLocation W15450768221 @default.
• W1545076822 hasRelatedWork W2029627918 @default.
• W1545076822 hasRelatedWork W2264187340 @default.
• W1545076822 hasRelatedWork W2351347946 @default.
• W1545076822 hasRelatedWork W2355838360 @default.
• W1545076822 hasRelatedWork W2360988011 @default.
• W1545076822 hasRelatedWork W2367964859 @default.
• W1545076822 hasRelatedWork W2373738424 @default.
• W1545076822 hasRelatedWork W2375240213 @default.
• W1545076822 hasRelatedWork W2384283330 @default.
• W1545076822 hasRelatedWork W2397306319 @default.
• W1545076822 hasVolume "15" @default.

• next