FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1545358907> ?p ?o ?g. }

• W1545358907 abstract "In drug resistant, especially multi-drug resistant (MDR) tuberculosis, fluoroquinolones (FQs) are used as second line drugs. However, the incidence of FQ-resistant Mycobacterium tuberculosis is rapidly increasing which may be due to extensive use of FQs in the treatment of various other diseases. The most important known mechanism i.e., gyrA mutation in FQ resistance is not observed in a significant proportion of FQ resistant M. tuberculosis isolates suggesting that the resistance may be because of other mechanisms such as an active drug efflux pump. In this study we evaluated the role of the efflux pumps in quinolone resistance by using various inhibitors such as carbonyl cyanide m-chlorophenyl hydrazone (CCCP), 2,4-dinitrophenol (DNP) and verapamil, in clinical isolates of M. tuberculosis.A total of 55 M. tuberculosis clinical isolates [45 ofloxacin (OFL) resistant and 10 ofloxacin sensitive] were tested by Resazurin microtitre assay (REMA) to observe the changes in ofloxacin minimum inhibitory concentration (MIC) levels in presence of efflux inhibitors as compared to control (without efflux inhibitor).The MIC levels of OFL showed 2-8 folds reduction in presence of CCCP (16/45; 35.5%), verapamil (24/45; 53.3%) and DNP (21/45; 46.6%) while in case of isolates identified as OFL sensitive these did not show any effect on ofloxacin MICs. In 11 of 45 (24.5%) isolates change in MIC levels was observed with all the three inhibitors. Overall 30 (66.6%) isolates had reduction in OFL MIC after treatment with these inhibitors. A total of eight isolates were sequenced for gyrA gene, of which, seven (87.5%) showed known mutations. Of the eight sequenced isolates, seven (87.5%) showed 2 to 8 fold change in MIC in presence of efflux inhibitors.Our findings suggest the involvement of active efflux pumps of both Major Facilitator Super Family (MFS) family (inhibited by CCCP and DNP) and ATP Binding Cassette (ABC) transporters (inhibited by verapamil) in the development of OFL resistance in M. tuberculosis isolates. Epidemiological significance of these findings needs to be determined in prospective studies with appropriate number of samples/isolates." @default.
• W1545358907 created "2016-06-24" @default.
• W1545358907 creator A5002511995 @default.
• W1545358907 creator A5005098851 @default.
• W1545358907 creator A5013988963 @default.
• W1545358907 creator A5019627093 @default.
• W1545358907 creator A5046737772 @default.
• W1545358907 creator A5055812247 @default.
• W1545358907 creator A5057041932 @default.
• W1545358907 creator A5057116586 @default.
• W1545358907 creator A5058780312 @default.
• W1545358907 creator A5068850222 @default.
• W1545358907 creator A5069114772 @default.
• W1545358907 creator A5089290417 @default.
• W1545358907 date "2011-05-01" @default.
• W1545358907 modified "2023-09-23" @default.
• W1545358907 title "Effect of efflux pump inhibitors on drug susceptibility of ofloxacin resistant Mycobacterium tuberculosis isolates." @default.
• W1545358907 cites W1884882993 @default.
• W1545358907 cites W1966126698 @default.
• W1545358907 cites W1982168502 @default.
• W1545358907 cites W2008858822 @default.
• W1545358907 cites W2014144663 @default.
• W1545358907 cites W2057498351 @default.
• W1545358907 cites W2058027101 @default.
• W1545358907 cites W2100378493 @default.
• W1545358907 cites W2121649640 @default.
• W1545358907 cites W2127776703 @default.
• W1545358907 cites W2129759335 @default.
• W1545358907 cites W2132987387 @default.
• W1545358907 cites W2140509482 @default.
• W1545358907 cites W2142392294 @default.
• W1545358907 cites W2156630451 @default.
• W1545358907 cites W2170172427 @default.
• W1545358907 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3121286" @default.
• W1545358907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21623040" @default.
• W1545358907 hasPublicationYear "2011" @default.
• W1545358907 type Work @default.
• W1545358907 sameAs 1545358907 @default.
• W1545358907 citedByCount "31" @default.
• W1545358907 countsByYear W15453589072013 @default.
• W1545358907 countsByYear W15453589072014 @default.
• W1545358907 countsByYear W15453589072015 @default.
• W1545358907 countsByYear W15453589072016 @default.
• W1545358907 countsByYear W15453589072017 @default.
• W1545358907 countsByYear W15453589072019 @default.
• W1545358907 countsByYear W15453589072021 @default.
• W1545358907 countsByYear W15453589072022 @default.
• W1545358907 countsByYear W15453589072023 @default.
• W1545358907 crossrefType "journal-article" @default.
• W1545358907 hasAuthorship W1545358907A5002511995 @default.
• W1545358907 hasAuthorship W1545358907A5005098851 @default.
• W1545358907 hasAuthorship W1545358907A5013988963 @default.
• W1545358907 hasAuthorship W1545358907A5019627093 @default.
• W1545358907 hasAuthorship W1545358907A5046737772 @default.
• W1545358907 hasAuthorship W1545358907A5055812247 @default.
• W1545358907 hasAuthorship W1545358907A5057041932 @default.
• W1545358907 hasAuthorship W1545358907A5057116586 @default.
• W1545358907 hasAuthorship W1545358907A5058780312 @default.
• W1545358907 hasAuthorship W1545358907A5068850222 @default.
• W1545358907 hasAuthorship W1545358907A5069114772 @default.
• W1545358907 hasAuthorship W1545358907A5089290417 @default.
• W1545358907 hasConcept C104317684 @default.
• W1545358907 hasConcept C114851261 @default.
• W1545358907 hasConcept C142724271 @default.
• W1545358907 hasConcept C159047783 @default.
• W1545358907 hasConcept C167125095 @default.
• W1545358907 hasConcept C200082930 @default.
• W1545358907 hasConcept C2777975735 @default.
• W1545358907 hasConcept C2778049240 @default.
• W1545358907 hasConcept C2778512257 @default.
• W1545358907 hasConcept C2779922871 @default.
• W1545358907 hasConcept C2780035454 @default.
• W1545358907 hasConcept C2781069245 @default.
• W1545358907 hasConcept C501593827 @default.
• W1545358907 hasConcept C54355233 @default.
• W1545358907 hasConcept C547475151 @default.
• W1545358907 hasConcept C71924100 @default.
• W1545358907 hasConcept C86803240 @default.
• W1545358907 hasConcept C89423630 @default.
• W1545358907 hasConcept C98274493 @default.
• W1545358907 hasConceptScore W1545358907C104317684 @default.
• W1545358907 hasConceptScore W1545358907C114851261 @default.
• W1545358907 hasConceptScore W1545358907C142724271 @default.
• W1545358907 hasConceptScore W1545358907C159047783 @default.
• W1545358907 hasConceptScore W1545358907C167125095 @default.
• W1545358907 hasConceptScore W1545358907C200082930 @default.
• W1545358907 hasConceptScore W1545358907C2777975735 @default.
• W1545358907 hasConceptScore W1545358907C2778049240 @default.
• W1545358907 hasConceptScore W1545358907C2778512257 @default.
• W1545358907 hasConceptScore W1545358907C2779922871 @default.
• W1545358907 hasConceptScore W1545358907C2780035454 @default.
• W1545358907 hasConceptScore W1545358907C2781069245 @default.
• W1545358907 hasConceptScore W1545358907C501593827 @default.
• W1545358907 hasConceptScore W1545358907C54355233 @default.
• W1545358907 hasConceptScore W1545358907C547475151 @default.
• W1545358907 hasConceptScore W1545358907C71924100 @default.
• W1545358907 hasConceptScore W1545358907C86803240 @default.
• W1545358907 hasConceptScore W1545358907C89423630 @default.
• W1545358907 hasConceptScore W1545358907C98274493 @default.
• W1545358907 hasLocation W15453589071 @default.

• next