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- W1545496749 abstract "Abstract N esidiocoris tenuis ( H eteroptera: Miridae) is a predator of some major pests of S olanaceae crops, yet it is scarcely used in biological control because it also feeds on plants and may damage crops. The study of N . tenuis biology may promote the ability to use it as a biological control agent. Because N . tenuis , like some other insect taxa, oviposits into plant tissues, its eggs are hard to detect. This limits our ability to study N . tenuis – plant interactions. We therefore looked for a staining method for plant‐embedded eggs, which will allow their detection within tomato plants, a common host of N . tenuis . We first used lactophenol solution with acid fuchsin to stain eggs inside tomato foliage. Because of the high toxicity of lactophenol, we later substituted lactophenol with a lactoglycerol solution, which was found to be similarly efficient. Five minutes immersion in the staining solution at 80°C followed by a two‐minute soak in hot water made the eggs stain deep red, while the foliage became transparent and was stained weak red. Eggs within leaves were easily visible under 10–30× magnification with sub‐stage lighting; top‐lighting was needed for the detection of eggs embedded in less‐transparent tissues such as stems. This rapid staining method improves the ability to study some important biological aspects of N . tenuis , such as its fecundity. Also, the elimination of phenol made the method cheaper and safer to use. Finally, this method may be adapted for other arthropod–plant systems." @default.
- W1545496749 created "2016-06-24" @default.
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- W1545496749 date "2015-02-02" @default.
- W1545496749 modified "2023-10-05" @default.
- W1545496749 title "Visualizing eggs of<i>Nesidiocoris tenuis</i>(Heteroptera: Miridae) embedded in tomato plant tissues" @default.
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- W1545496749 doi "https://doi.org/10.1111/ens.12125" @default.
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