Matches in SemOpenAlex for { <https://semopenalex.org/work/W1545707013> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1545707013 endingPage "33" @default.
- W1545707013 startingPage "29" @default.
- W1545707013 abstract "The rickettsia Anaplasma marginale is considered the main agent of bovine anaplasmosis. Due the nonspecific clinical signs of the anaplasmosis, the diagnosis of infection depends of laboratory confirmation. In recent years, molecular diagnostic methods have been used to detect A. marginale in cattle. However, the existence of a large number of assays of different sensitivity and cost makes the choice of an appropriate test difficult. In the present study, a real-time Polymerase Chain Reaction (PCR) based on the msp5 target gene was quantitatively assessed and compared to an end point PCR. Both reactions were subjected to sensitivity and specificity evaluation using plasmid DNA and samples from cattle experimentally infected with A. marginale. A comparative field trial of the tests was carried out using samples of cattle from a stable enzootic area for A. marginale. The real-time PCR showed a higher sensitivity than the end point PCR. This reaction (i.e. real-time PCR) was able to detect one copy of the msp5 gene in 100 ηg of plasmidial DNA, and more than 80% of its results were positive among experimentally infected animals seven days after infection. In addition, based on in silico analysis, the real-time PCR evaluated in the present study appears to be useful for the detection of A. ovis." @default.
- W1545707013 created "2016-06-24" @default.
- W1545707013 creator A5012235697 @default.
- W1545707013 creator A5018051435 @default.
- W1545707013 creator A5030068400 @default.
- W1545707013 date "2014-01-01" @default.
- W1545707013 modified "2023-09-23" @default.
- W1545707013 title "Molecular diagnosis of Anaplasma marginale in cattle: quantitative evaluation of a real-time PCR (Polymerase Chain Reaction) based on msp5 gene" @default.
- W1545707013 cites W1604842667 @default.
- W1545707013 cites W1966741741 @default.
- W1545707013 cites W1995383563 @default.
- W1545707013 cites W1998094280 @default.
- W1545707013 cites W2012955604 @default.
- W1545707013 cites W2014659929 @default.
- W1545707013 cites W2048256578 @default.
- W1545707013 cites W2048803559 @default.
- W1545707013 cites W2054607887 @default.
- W1545707013 cites W2097825407 @default.
- W1545707013 cites W2100620639 @default.
- W1545707013 cites W2102109116 @default.
- W1545707013 cites W2102801292 @default.
- W1545707013 cites W2105725017 @default.
- W1545707013 cites W2122367683 @default.
- W1545707013 cites W2131184698 @default.
- W1545707013 cites W2148844706 @default.
- W1545707013 cites W2160380480 @default.
- W1545707013 doi "https://doi.org/10.1590/s0100-736x2014000100005" @default.
- W1545707013 hasPublicationYear "2014" @default.
- W1545707013 type Work @default.
- W1545707013 sameAs 1545707013 @default.
- W1545707013 citedByCount "4" @default.
- W1545707013 countsByYear W15457070132016 @default.
- W1545707013 countsByYear W15457070132017 @default.
- W1545707013 countsByYear W15457070132019 @default.
- W1545707013 crossrefType "journal-article" @default.
- W1545707013 hasAuthorship W1545707013A5012235697 @default.
- W1545707013 hasAuthorship W1545707013A5018051435 @default.
- W1545707013 hasAuthorship W1545707013A5030068400 @default.
- W1545707013 hasBestOaLocation W15457070131 @default.
- W1545707013 hasConcept C104317684 @default.
- W1545707013 hasConcept C153911025 @default.
- W1545707013 hasConcept C159047783 @default.
- W1545707013 hasConcept C22744801 @default.
- W1545707013 hasConcept C2777116013 @default.
- W1545707013 hasConcept C2779620486 @default.
- W1545707013 hasConcept C2781329793 @default.
- W1545707013 hasConcept C48023723 @default.
- W1545707013 hasConcept C49105822 @default.
- W1545707013 hasConcept C54355233 @default.
- W1545707013 hasConcept C86803240 @default.
- W1545707013 hasConceptScore W1545707013C104317684 @default.
- W1545707013 hasConceptScore W1545707013C153911025 @default.
- W1545707013 hasConceptScore W1545707013C159047783 @default.
- W1545707013 hasConceptScore W1545707013C22744801 @default.
- W1545707013 hasConceptScore W1545707013C2777116013 @default.
- W1545707013 hasConceptScore W1545707013C2779620486 @default.
- W1545707013 hasConceptScore W1545707013C2781329793 @default.
- W1545707013 hasConceptScore W1545707013C48023723 @default.
- W1545707013 hasConceptScore W1545707013C49105822 @default.
- W1545707013 hasConceptScore W1545707013C54355233 @default.
- W1545707013 hasConceptScore W1545707013C86803240 @default.
- W1545707013 hasIssue "1" @default.
- W1545707013 hasLocation W15457070131 @default.
- W1545707013 hasLocation W15457070132 @default.
- W1545707013 hasLocation W15457070133 @default.
- W1545707013 hasLocation W15457070134 @default.
- W1545707013 hasOpenAccess W1545707013 @default.
- W1545707013 hasPrimaryLocation W15457070131 @default.
- W1545707013 hasRelatedWork W1545707013 @default.
- W1545707013 hasRelatedWork W1811249054 @default.
- W1545707013 hasRelatedWork W2039292541 @default.
- W1545707013 hasRelatedWork W2055795193 @default.
- W1545707013 hasRelatedWork W2114319572 @default.
- W1545707013 hasRelatedWork W2119335634 @default.
- W1545707013 hasRelatedWork W2154651631 @default.
- W1545707013 hasRelatedWork W2236397427 @default.
- W1545707013 hasRelatedWork W2383054771 @default.
- W1545707013 hasRelatedWork W282409040 @default.
- W1545707013 hasVolume "34" @default.
- W1545707013 isParatext "false" @default.
- W1545707013 isRetracted "false" @default.
- W1545707013 magId "1545707013" @default.
- W1545707013 workType "article" @default.