FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1546151101> ?p ?o ?g. }

• W1546151101 endingPage "116" @default.
• W1546151101 startingPage "100" @default.
• W1546151101 abstract "The present study tends to  test the validity of REMI-SMGT and to evaluate the efficiency of REMI-SMGT on generating other mammalian species rather than the sheep that made only by one group and to observe the possibility of doing so by using rabbits as a model for this approach and also to reduce the cost of REMI-SMGT by substituting liposomes and highly cost effective media with a high efficient, non-cost effective substitute. Direct protective relationship of liposome with DNA and seminal fluid was identified compared with DMSO.  While different treatments (linearized DNA – restriction enzyme – liposome complex, DNA – restriction enzyme – DMSO complex, DNA –DMSO complex, DNA –liposome complex, and even naked DNA) were all found to be successful to internalize inside the head of the sperm according to PCR results, only three (one by restriction enzyme – liposome treatment and two by restriction enzyme – DMSO treatment) out of fourteen new born babies were found to be transgenic by PCR. Despite the absolute ability of exogenous DNA to be internalized inside rabbit’s sperm head only few percent of transgenic babies were obtained. This may not reflect the weakness of restriction enzyme mediated transgenesis technique itself but it reflects the inability of recombinant sperm to fertilize superovulated oocyte compared with their normal counterparts. Comparable results were found between liposome and DMSO treatment which may reflect direct relationship of DMSO with the cell membrane instead of with the exogenous DNA itself as what is found with liposome. Key words : REMI-SMGT , PCR , DNA – restriction enzyme , DMSO" @default.
• W1546151101 created "2016-06-24" @default.
• W1546151101 creator A5015310539 @default.
• W1546151101 creator A5024464167 @default.
• W1546151101 creator A5030657767 @default.
• W1546151101 creator A5075192075 @default.
• W1546151101 date "2013-01-01" @default.
• W1546151101 modified "2023-09-26" @default.
• W1546151101 title "The Efficiency of Transgenesis by Restriction Enzyme Mediated Integration - Sperm Mediated Gene Transfer (REMI-SMGT)" @default.
• W1546151101 cites W1563072749 @default.
• W1546151101 cites W1572202589 @default.
• W1546151101 cites W1595002251 @default.
• W1546151101 cites W1968283357 @default.
• W1546151101 cites W1973848209 @default.
• W1546151101 cites W1973879730 @default.
• W1546151101 cites W1977107900 @default.
• W1546151101 cites W1985513108 @default.
• W1546151101 cites W1988473354 @default.
• W1546151101 cites W1989293984 @default.
• W1546151101 cites W2003918704 @default.
• W1546151101 cites W2008841569 @default.
• W1546151101 cites W2015288952 @default.
• W1546151101 cites W2020302862 @default.
• W1546151101 cites W2024522705 @default.
• W1546151101 cites W2033193915 @default.
• W1546151101 cites W2046959697 @default.
• W1546151101 cites W2050688882 @default.
• W1546151101 cites W2051038137 @default.
• W1546151101 cites W2059727398 @default.
• W1546151101 cites W2059733640 @default.
• W1546151101 cites W2061131538 @default.
• W1546151101 cites W2064444102 @default.
• W1546151101 cites W2073035527 @default.
• W1546151101 cites W2078657342 @default.
• W1546151101 cites W2080465807 @default.
• W1546151101 cites W2081326288 @default.
• W1546151101 cites W2081404678 @default.
• W1546151101 cites W2088485592 @default.
• W1546151101 cites W2101545638 @default.
• W1546151101 cites W2112265114 @default.
• W1546151101 cites W2127068865 @default.
• W1546151101 cites W2128537239 @default.
• W1546151101 cites W2136475641 @default.
• W1546151101 cites W2139193846 @default.
• W1546151101 cites W2146052752 @default.
• W1546151101 cites W2148538111 @default.
• W1546151101 cites W2160064083 @default.
• W1546151101 cites W2162400872 @default.
• W1546151101 cites W2169454898 @default.
• W1546151101 cites W2295926973 @default.
• W1546151101 cites W2300770957 @default.
• W1546151101 cites W2411988450 @default.
• W1546151101 cites W2471729359 @default.
• W1546151101 cites W3201971479 @default.
• W1546151101 cites W585622130 @default.
• W1546151101 cites W2186951943 @default.
• W1546151101 hasPublicationYear "2013" @default.
• W1546151101 type Work @default.
• W1546151101 sameAs 1546151101 @default.
• W1546151101 citedByCount "0" @default.
• W1546151101 crossrefType "journal-article" @default.
• W1546151101 hasAuthorship W1546151101A5015310539 @default.
• W1546151101 hasAuthorship W1546151101A5024464167 @default.
• W1546151101 hasAuthorship W1546151101A5030657767 @default.
• W1546151101 hasAuthorship W1546151101A5075192075 @default.
• W1546151101 hasConcept C102230213 @default.
• W1546151101 hasConcept C104317684 @default.
• W1546151101 hasConcept C128319531 @default.
• W1546151101 hasConcept C153911025 @default.
• W1546151101 hasConcept C181199279 @default.
• W1546151101 hasConcept C182420341 @default.
• W1546151101 hasConcept C185154212 @default.
• W1546151101 hasConcept C195856716 @default.
• W1546151101 hasConcept C22744801 @default.
• W1546151101 hasConcept C2778093475 @default.
• W1546151101 hasConcept C2781087480 @default.
• W1546151101 hasConcept C2994278967 @default.
• W1546151101 hasConcept C40767141 @default.
• W1546151101 hasConcept C54355233 @default.
• W1546151101 hasConcept C552990157 @default.
• W1546151101 hasConcept C55493867 @default.
• W1546151101 hasConcept C58301748 @default.
• W1546151101 hasConcept C73247094 @default.
• W1546151101 hasConcept C86803240 @default.
• W1546151101 hasConcept C87073359 @default.
• W1546151101 hasConceptScore W1546151101C102230213 @default.
• W1546151101 hasConceptScore W1546151101C104317684 @default.
• W1546151101 hasConceptScore W1546151101C128319531 @default.
• W1546151101 hasConceptScore W1546151101C153911025 @default.
• W1546151101 hasConceptScore W1546151101C181199279 @default.
• W1546151101 hasConceptScore W1546151101C182420341 @default.
• W1546151101 hasConceptScore W1546151101C185154212 @default.
• W1546151101 hasConceptScore W1546151101C195856716 @default.
• W1546151101 hasConceptScore W1546151101C22744801 @default.
• W1546151101 hasConceptScore W1546151101C2778093475 @default.
• W1546151101 hasConceptScore W1546151101C2781087480 @default.
• W1546151101 hasConceptScore W1546151101C2994278967 @default.
• W1546151101 hasConceptScore W1546151101C40767141 @default.

• next