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- W1546608122 abstract "Using a common temperate herbaceous terrestrial orchid from Australia (Caladenia latifolia) this study investigated 19 asymbiotic media variations comprising four commonly used basal media [half-strength Murashige and Skoog (½MS), Knudson C (KC), Pa5, and Vacin and Went (VW), with combinations of the plant growth regulators 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) or coconut water (CW) and compared their performance with germination on a standard symbiotic germination medium, oatmeal agar (OMA). Percentage germination of seeds every 2 weeks for a total of 8 weeks (five replicates per treatment), time to germination, and growth and development phases in seedlings were recorded. ½MS with 5% (v/v) fresh CW delivered 93% germination, with seedling vigour and development indistinguishable from OMA (95% germination). The same protocol was applied to a further ten species (including the endangered Caladenia huegelii), demonstrating high asymbiotic germination performance (60–93%) across a wide phylogenetic range of terrestrial orchid species." @default.
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- W1546608122 date "2014-11-12" @default.
- W1546608122 modified "2023-10-16" @default.
- W1546608122 title "<i>In vitro</i>propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination" @default.
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- W1546608122 doi "https://doi.org/10.1111/boj.12216" @default.
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