FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1547253301> ?p ?o ?g. }

• W1547253301 endingPage "321" @default.
• W1547253301 startingPage "305" @default.
• W1547253301 abstract "Growth factor receptors are present in the plasma membrane of resting cells as monomers or (pre)dimers. Ligand binding results in higher-order oligomerization of ligand–receptor complexes. To study the regulation of receptor clustering, several experimental techniques have been developed in the last decades. However, many involve invasive approaches that are likely to disturb the integrity of the membrane, thereby affecting receptor interactions. In this chapter, we describe the use of a noninvasive approach to study receptor dimerization and oligomerization. This method is based upon the Förster energy transfer between identical adjacent fluorescent proteins (homo-FRET) and is determined by analyzing the change in fluorescence anisotropy. Homo-FRET takes place within a distance of 10 nm, making this an excellent approach for studying receptor–receptor interactions in intact cells. After excitation of monomeric GFP (mGFP) with polarized light, limiting anisotropy values (rinf) of the emitted light are determined, where proteins with known cluster sizes are used as references. Dimerization and oligomerization of the epidermal growth factor receptor (EGFR) in response to ligand binding is determined by using receptors that have been fused with mGFP at their C-terminus. In this chapter, we describe the involved technology and discuss the feasibility of homo-FRET experiments for the determination of cluster sizes of growth factor receptors like EGFR." @default.
• W1547253301 created "2016-06-24" @default.
• W1547253301 creator A5009585600 @default.
• W1547253301 creator A5013056108 @default.
• W1547253301 creator A5022149699 @default.
• W1547253301 creator A5038761596 @default.
• W1547253301 creator A5068827641 @default.
• W1547253301 date "2013-01-01" @default.
• W1547253301 modified "2023-09-26" @default.
• W1547253301 title "Analysis of EGF Receptor Oligomerization by Homo-FRET" @default.
• W1547253301 cites W1964321121 @default.
• W1547253301 cites W1976002101 @default.
• W1547253301 cites W1979518220 @default.
• W1547253301 cites W1979566484 @default.
• W1547253301 cites W1980735842 @default.
• W1547253301 cites W1990222254 @default.
• W1547253301 cites W1998547071 @default.
• W1547253301 cites W2000638372 @default.
• W1547253301 cites W2014565808 @default.
• W1547253301 cites W2019772485 @default.
• W1547253301 cites W2021628061 @default.
• W1547253301 cites W2027856540 @default.
• W1547253301 cites W2032359283 @default.
• W1547253301 cites W2043911313 @default.
• W1547253301 cites W2055864348 @default.
• W1547253301 cites W2065010237 @default.
• W1547253301 cites W2068279659 @default.
• W1547253301 cites W2081215733 @default.
• W1547253301 cites W2087323736 @default.
• W1547253301 cites W2099203391 @default.
• W1547253301 cites W2102273931 @default.
• W1547253301 cites W2112915535 @default.
• W1547253301 cites W2116202008 @default.
• W1547253301 cites W2122706875 @default.
• W1547253301 cites W2123297555 @default.
• W1547253301 cites W2135897043 @default.
• W1547253301 cites W2147493949 @default.
• W1547253301 cites W2151964889 @default.
• W1547253301 cites W2153559476 @default.
• W1547253301 cites W2169085523 @default.
• W1547253301 cites W2171895487 @default.
• W1547253301 doi "https://doi.org/10.1016/b978-0-12-408143-7.00016-5" @default.
• W1547253301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24143984" @default.
• W1547253301 hasPublicationYear "2013" @default.
• W1547253301 type Work @default.
• W1547253301 sameAs 1547253301 @default.
• W1547253301 citedByCount "10" @default.
• W1547253301 countsByYear W15472533012014 @default.
• W1547253301 countsByYear W15472533012016 @default.
• W1547253301 countsByYear W15472533012018 @default.
• W1547253301 countsByYear W15472533012019 @default.
• W1547253301 countsByYear W15472533012021 @default.
• W1547253301 crossrefType "book-chapter" @default.
• W1547253301 hasAuthorship W1547253301A5009585600 @default.
• W1547253301 hasAuthorship W1547253301A5013056108 @default.
• W1547253301 hasAuthorship W1547253301A5022149699 @default.
• W1547253301 hasAuthorship W1547253301A5038761596 @default.
• W1547253301 hasAuthorship W1547253301A5068827641 @default.
• W1547253301 hasConcept C111357860 @default.
• W1547253301 hasConcept C116569031 @default.
• W1547253301 hasConcept C121332964 @default.
• W1547253301 hasConcept C12554922 @default.
• W1547253301 hasConcept C166940927 @default.
• W1547253301 hasConcept C170493617 @default.
• W1547253301 hasConcept C178790620 @default.
• W1547253301 hasConcept C185592680 @default.
• W1547253301 hasConcept C2779438470 @default.
• W1547253301 hasConcept C41625074 @default.
• W1547253301 hasConcept C521977710 @default.
• W1547253301 hasConcept C55493867 @default.
• W1547253301 hasConcept C62520636 @default.
• W1547253301 hasConcept C86803240 @default.
• W1547253301 hasConcept C91881484 @default.
• W1547253301 hasConcept C96305047 @default.
• W1547253301 hasConceptScore W1547253301C111357860 @default.
• W1547253301 hasConceptScore W1547253301C116569031 @default.
• W1547253301 hasConceptScore W1547253301C121332964 @default.
• W1547253301 hasConceptScore W1547253301C12554922 @default.
• W1547253301 hasConceptScore W1547253301C166940927 @default.
• W1547253301 hasConceptScore W1547253301C170493617 @default.
• W1547253301 hasConceptScore W1547253301C178790620 @default.
• W1547253301 hasConceptScore W1547253301C185592680 @default.
• W1547253301 hasConceptScore W1547253301C2779438470 @default.
• W1547253301 hasConceptScore W1547253301C41625074 @default.
• W1547253301 hasConceptScore W1547253301C521977710 @default.
• W1547253301 hasConceptScore W1547253301C55493867 @default.
• W1547253301 hasConceptScore W1547253301C62520636 @default.
• W1547253301 hasConceptScore W1547253301C86803240 @default.
• W1547253301 hasConceptScore W1547253301C91881484 @default.
• W1547253301 hasConceptScore W1547253301C96305047 @default.
• W1547253301 hasLocation W15472533011 @default.
• W1547253301 hasLocation W15472533012 @default.
• W1547253301 hasOpenAccess W1547253301 @default.
• W1547253301 hasPrimaryLocation W15472533011 @default.
• W1547253301 hasRelatedWork W1547253301 @default.
• W1547253301 hasRelatedWork W1968817888 @default.
• W1547253301 hasRelatedWork W1972298453 @default.
• W1547253301 hasRelatedWork W2035164663 @default.

• next