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- W1547306214 abstract "Publisher Summary This chapter describes the detection, assay, and isolation of allene oxide synthase. The enzyme(s) now commonly referred to as allene oxide synthase (AOS), CYP74 was reported as an activity in flaxseed that metabolized linoleic acid hydroperoxide to an α-ketol derivative. Other associated products from unsaturated fatty acid hydroperoxides are γ-ketols and cyclopentenone fatty acids. These derivatives arise by hydrolysis and rearrangements of the initial enzymic product, the very unstable allene oxide. The assay for detection of allene oxide synthase, that is, UV assay, is easily carried out using the reduction in UV absorbance associated with the formation and instantaneous hydrolysis of the allene oxide mainly to non-UV-absorbing α-ketols. The purification procedure was optimized by monitoring enzymatic activity through several open column and fast protein liquid chromatography (FPLC) methods. The method may be applicable to purification of allene oxide synthase from other plant sources. In addition to the flaxseed AOS, purification of the AOS from maize was achieved and N -terminal and CNBr peptide sequences were obtained using the same chromatographic steps." @default.
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- W1547306214 date "1996-01-01" @default.
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- W1547306214 title "[28] Detection, assay, and isolation of allene oxide synthase" @default.
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- W1547306214 doi "https://doi.org/10.1016/s0076-6879(96)72030-x" @default.
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