Matches in SemOpenAlex for { <https://semopenalex.org/work/W1547738863> ?p ?o ?g. }
- W1547738863 endingPage "2122" @default.
- W1547738863 startingPage "2113" @default.
- W1547738863 abstract "Abstract Arp2/3 complex is believed to induce de novo nucleation of actin filaments at the edge of motile cells downstream of WASp family proteins. In this study, the signaling pathways leading to Arp2/3 complex activation, actin assembly, and shape change were investigated in platelets isolated from patients with Wiskott-Aldrich Syndrome (WAS), that is, who lack WASp, and in WASp-deficient mouse platelets. WASp-deficient human and mouse platelets elaborate filopodia, spread lamellae, and assemble actin, identical to control WASp-expressing platelets. Human platelets contain 2 μM Arp2/3 complex, or 8600 molecules/cell. Arp2/3 complex redistributes to the edge of the lamellae and to the Triton X-100–insoluble actin cytoskeleton of activated WASp-deficient platelets. Furthermore, the C-terminal CA domain of N-WASp, which sequesters Arp2/3 complex, inhibits by half the actin nucleation capacity of octylglucoside-permeabilized and activated WAS platelets, similar to its effect in WASp-expressing cells. Along with WASp, platelets express WAVE-2 as a physiologic activator of Arp2/3 complex and a small amount of N-WASp. Taken together, our findings show that platelets activate Arp2/3 complex, assemble actin, and change shape in the absence of WASp, indicating a more specialized role for WASp in these cells." @default.
- W1547738863 created "2016-06-24" @default.
- W1547738863 creator A5008837698 @default.
- W1547738863 creator A5019757442 @default.
- W1547738863 creator A5023521073 @default.
- W1547738863 creator A5071191923 @default.
- W1547738863 date "2002-09-15" @default.
- W1547738863 modified "2023-10-03" @default.
- W1547738863 title "Normal Arp2/3 complex activation in platelets lacking WASp" @default.
- W1547738863 cites W1498435062 @default.
- W1547738863 cites W1518379473 @default.
- W1547738863 cites W1519901358 @default.
- W1547738863 cites W1520402351 @default.
- W1547738863 cites W1524321385 @default.
- W1547738863 cites W1524475817 @default.
- W1547738863 cites W1538520630 @default.
- W1547738863 cites W1561743635 @default.
- W1547738863 cites W1567813092 @default.
- W1547738863 cites W1571919210 @default.
- W1547738863 cites W1575892864 @default.
- W1547738863 cites W1593929303 @default.
- W1547738863 cites W1601128253 @default.
- W1547738863 cites W1630317533 @default.
- W1547738863 cites W1635520329 @default.
- W1547738863 cites W1658693572 @default.
- W1547738863 cites W1965234951 @default.
- W1547738863 cites W1965964686 @default.
- W1547738863 cites W1969172568 @default.
- W1547738863 cites W1969563780 @default.
- W1547738863 cites W1971557011 @default.
- W1547738863 cites W1975497127 @default.
- W1547738863 cites W1977513008 @default.
- W1547738863 cites W1982641617 @default.
- W1547738863 cites W1995757732 @default.
- W1547738863 cites W2006996074 @default.
- W1547738863 cites W2010781630 @default.
- W1547738863 cites W2016253390 @default.
- W1547738863 cites W2018317768 @default.
- W1547738863 cites W2020422209 @default.
- W1547738863 cites W2021329171 @default.
- W1547738863 cites W2025218356 @default.
- W1547738863 cites W2027810531 @default.
- W1547738863 cites W2031183440 @default.
- W1547738863 cites W2031874417 @default.
- W1547738863 cites W2032667632 @default.
- W1547738863 cites W2033619721 @default.
- W1547738863 cites W2038088851 @default.
- W1547738863 cites W2039325880 @default.
- W1547738863 cites W2042049079 @default.
- W1547738863 cites W2043763151 @default.
- W1547738863 cites W2045829104 @default.
- W1547738863 cites W2045923054 @default.
- W1547738863 cites W2059161447 @default.
- W1547738863 cites W2061288879 @default.
- W1547738863 cites W2072837740 @default.
- W1547738863 cites W2076586496 @default.
- W1547738863 cites W2078384542 @default.
- W1547738863 cites W2079099921 @default.
- W1547738863 cites W2095393268 @default.
- W1547738863 cites W2096791952 @default.
- W1547738863 cites W2096873347 @default.
- W1547738863 cites W2100990664 @default.
- W1547738863 cites W2105789974 @default.
- W1547738863 cites W2107625881 @default.
- W1547738863 cites W2110726109 @default.
- W1547738863 cites W2114560611 @default.
- W1547738863 cites W2121889667 @default.
- W1547738863 cites W2122213969 @default.
- W1547738863 cites W2122782850 @default.
- W1547738863 cites W2136611642 @default.
- W1547738863 cites W2139094884 @default.
- W1547738863 cites W2143442358 @default.
- W1547738863 cites W2148334175 @default.
- W1547738863 cites W2153482320 @default.
- W1547738863 cites W2157815908 @default.
- W1547738863 cites W2166520458 @default.
- W1547738863 cites W2167146326 @default.
- W1547738863 cites W2168537923 @default.
- W1547738863 cites W2183670135 @default.
- W1547738863 cites W2289617846 @default.
- W1547738863 cites W4233571944 @default.
- W1547738863 cites W4237603521 @default.
- W1547738863 cites W4250305484 @default.
- W1547738863 cites W86341414 @default.
- W1547738863 doi "https://doi.org/10.1182/blood.v100.6.2113" @default.
- W1547738863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12200375" @default.
- W1547738863 hasPublicationYear "2002" @default.
- W1547738863 type Work @default.
- W1547738863 sameAs 1547738863 @default.
- W1547738863 citedByCount "65" @default.
- W1547738863 countsByYear W15477388632013 @default.
- W1547738863 countsByYear W15477388632014 @default.
- W1547738863 countsByYear W15477388632016 @default.
- W1547738863 countsByYear W15477388632017 @default.
- W1547738863 countsByYear W15477388632018 @default.
- W1547738863 countsByYear W15477388632019 @default.
- W1547738863 countsByYear W15477388632020 @default.
- W1547738863 countsByYear W15477388632021 @default.