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- W1548169964 abstract "A multiplex PCR assay was developed using two primer sets for the identification and differentiation ofCampylobacter coliandCampylobacter jejuni. Primer Set I amplifies a 460-bp fragment present inC. coliandC. jejuni. Set II amplifies a 160-bp target unique toC. jejuni. When the assay was performed on reference strains, amplification ofC. coliyielded only the 460-bp fragment. Amplification ofC. jejunigenerated both the 160- and 460-bp fragments.Campylobacterfield strains (n=85) isolated from raw poultry were identified by PCR and by conventional biochemical methods. Species determination by the two methods agreed for 83 of the 85 isolates examined. By PCR, 23 were identified asC. coliand 62 asC. jejuni. One isolate was unidentifiable by biochemical testing. The PCR assay identified this isolate asC. coli. In addition, one strain which was identified asC. coliby biochemical testing was determined to beC. jejuniby PCR. The PCR assay offers an alternative to traditional biochemical typing methods for the identification and differentiation ofC. coliandC. jejuniisolated from poultry. It is accurate, simple to perform, and can be completed within 8 h." @default.
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- W1548169964 date "1997-06-01" @default.
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- W1548169964 title "Differentiation ofCampylobacter jejuniandCampylobacter coliby polymerase chain reaction" @default.
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- W1548169964 doi "https://doi.org/10.1006/mcpr.1997.0104" @default.
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