FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1548622561> ?p ?o ?g. }

• W1548622561 endingPage "54" @default.
• W1548622561 startingPage "47" @default.
• W1548622561 abstract "Golgi vesicles were isolated and purified from rat liver, in which the specific activities of glycosyltransferases (e.g. GM3:CMP-NeuAc sialyltransferase, GD3 synthase; GM3:UDP-GalNAc galactosaminyltransferase, GM2 synthase) were 50-60-times enriched relative to microsomes or total homogenate. Synthesis of gangliosides GM2 and GM1 in such Golgi vesicles is, in the absence of any detergents, stimulated 6-fold and 20-fold respectively by phosphatidylglycerol. Other phospholipids like phosphatidylethanolamine and phosphatidylserine are also significantly stimulatory. With 50 micrograms Golgi protein and 1 nmol UDP-GalNAc, optimal stimulation of GM2 synthase was obtained with 20 micrograms of phosphatidylglycerol and 7.5 nmol of the lipid acceptor GM3. Under the same experimental conditions this stimulation exceeds (by about 40%) that obtained with optimal amount (200 micrograms) of the detergent octylglucoside. Phosphatidylglycerol, on the other hand, has virtually no stimulatory activity on the synthesis of ganglioside GD3 either in the presence of Mg2+ or Mn2+, indicating that facilitation by phospholipid of GM3 transport into Golgi vesicles was not the basis of stimulation of GM2 synthesis. Tunicamycin inhibits the synthesis of gangliosides GM2 and GM1 in isolated Golgi vesicles, but only in the absence of detergents. In the presence of phosphatidylglycerol, GM2 synthesis, for example, was inhibited by 60% by 2 micrograms tunicamycin and more than 85% by 10 micrograms tunicamycin, per 50 micrograms Golgi membrane protein. The inhibition was stronger on GM1 synthesis: 85% with 2.5 micrograms of the antibiotic. The dependence on phosphatidylglycerol and the degree of inhibition by tunicamycin of the synthetic activities are strictly dependent on the intactness of the Golgi vesicles: both phenomena become increasingly less evident when the vesicles are pelleted, and frozen and thawed several times, and completely disappear when the vesicles are solubilized by detergents or disrupted by ultrasonication. Furthermore, tunicamycin inhibition is reversible by increased concentration of phosphatidylglycerol. All these results indicate that phosphatidylglycerol does not stimulate, and tunicamycin does not inhibit, the transferases themselves; rather, the two opposing effects might relate to carrier-mediated transport, e.g. of nucleotide sugars, across Golgi vesicles." @default.
• W1548622561 created "2016-06-24" @default.
• W1548622561 creator A5007309669 @default.
• W1548622561 creator A5021624903 @default.
• W1548622561 creator A5029025192 @default.
• W1548622561 creator A5072065214 @default.
• W1548622561 date "1983-07-01" @default.
• W1548622561 modified "2023-10-13" @default.
• W1548622561 title "Ganglioside Biosynthesis in Golgi Apparatus of Rat Liver. Stimulation by Phosphatidylglycerol and Inhibition by Tunicamycin" @default.
• W1548622561 cites W1482044739 @default.
• W1548622561 cites W1483635415 @default.
• W1548622561 cites W1501428107 @default.
• W1548622561 cites W1517330945 @default.
• W1548622561 cites W1536299500 @default.
• W1548622561 cites W1545396655 @default.
• W1548622561 cites W1551460886 @default.
• W1548622561 cites W1556337919 @default.
• W1548622561 cites W1559122076 @default.
• W1548622561 cites W1569504000 @default.
• W1548622561 cites W1575770083 @default.
• W1548622561 cites W1576620622 @default.
• W1548622561 cites W1595378945 @default.
• W1548622561 cites W1707394724 @default.
• W1548622561 cites W1775749144 @default.
• W1548622561 cites W1971338863 @default.
• W1548622561 cites W1980690858 @default.
• W1548622561 cites W1993029367 @default.
• W1548622561 cites W1995847875 @default.
• W1548622561 cites W1997164300 @default.
• W1548622561 cites W2000527155 @default.
• W1548622561 cites W2026276957 @default.
• W1548622561 cites W2041094173 @default.
• W1548622561 cites W2045682314 @default.
• W1548622561 cites W2062790386 @default.
• W1548622561 cites W2063048256 @default.
• W1548622561 cites W2070908410 @default.
• W1548622561 cites W2072773690 @default.
• W1548622561 cites W2181039790 @default.
• W1548622561 cites W2413451605 @default.
• W1548622561 doi "https://doi.org/10.1111/j.1432-1033.1983.tb07529.x" @default.
• W1548622561 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6861762" @default.
• W1548622561 hasPublicationYear "1983" @default.
• W1548622561 type Work @default.
• W1548622561 sameAs 1548622561 @default.
• W1548622561 citedByCount "66" @default.
• W1548622561 countsByYear W15486225612012 @default.
• W1548622561 countsByYear W15486225612018 @default.
• W1548622561 countsByYear W15486225612022 @default.
• W1548622561 crossrefType "journal-article" @default.
• W1548622561 hasAuthorship W1548622561A5007309669 @default.
• W1548622561 hasAuthorship W1548622561A5021624903 @default.
• W1548622561 hasAuthorship W1548622561A5029025192 @default.
• W1548622561 hasAuthorship W1548622561A5072065214 @default.
• W1548622561 hasConcept C119062480 @default.
• W1548622561 hasConcept C130316041 @default.
• W1548622561 hasConcept C139447449 @default.
• W1548622561 hasConcept C158617107 @default.
• W1548622561 hasConcept C185592680 @default.
• W1548622561 hasConcept C2776330855 @default.
• W1548622561 hasConcept C2777575526 @default.
• W1548622561 hasConcept C2778918659 @default.
• W1548622561 hasConcept C2779637612 @default.
• W1548622561 hasConcept C2780726299 @default.
• W1548622561 hasConcept C2781170229 @default.
• W1548622561 hasConcept C2910033705 @default.
• W1548622561 hasConcept C41625074 @default.
• W1548622561 hasConcept C55493867 @default.
• W1548622561 hasConcept C86803240 @default.
• W1548622561 hasConceptScore W1548622561C119062480 @default.
• W1548622561 hasConceptScore W1548622561C130316041 @default.
• W1548622561 hasConceptScore W1548622561C139447449 @default.
• W1548622561 hasConceptScore W1548622561C158617107 @default.
• W1548622561 hasConceptScore W1548622561C185592680 @default.
• W1548622561 hasConceptScore W1548622561C2776330855 @default.
• W1548622561 hasConceptScore W1548622561C2777575526 @default.
• W1548622561 hasConceptScore W1548622561C2778918659 @default.
• W1548622561 hasConceptScore W1548622561C2779637612 @default.
• W1548622561 hasConceptScore W1548622561C2780726299 @default.
• W1548622561 hasConceptScore W1548622561C2781170229 @default.
• W1548622561 hasConceptScore W1548622561C2910033705 @default.
• W1548622561 hasConceptScore W1548622561C41625074 @default.
• W1548622561 hasConceptScore W1548622561C55493867 @default.
• W1548622561 hasConceptScore W1548622561C86803240 @default.
• W1548622561 hasIssue "1" @default.
• W1548622561 hasLocation W15486225611 @default.
• W1548622561 hasLocation W15486225612 @default.
• W1548622561 hasOpenAccess W1548622561 @default.
• W1548622561 hasPrimaryLocation W15486225611 @default.
• W1548622561 hasRelatedWork W1548622561 @default.
• W1548622561 hasRelatedWork W1973900880 @default.
• W1548622561 hasRelatedWork W1990198778 @default.
• W1548622561 hasRelatedWork W2004878901 @default.
• W1548622561 hasRelatedWork W2008804874 @default.
• W1548622561 hasRelatedWork W2051187845 @default.
• W1548622561 hasRelatedWork W2059583682 @default.
• W1548622561 hasRelatedWork W2076570873 @default.
• W1548622561 hasRelatedWork W2086802687 @default.
• W1548622561 hasRelatedWork W2100317204 @default.

• next