FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1548749287> ?p ?o ?g. }

• W1548749287 abstract "Publisher Summary This chapter describes the methods used to study the kinetics and regulation of some protein-protein interactions involved in the phototransduction cascade. The first step in an experiment using a BIACORE instrument is to immobilize the ligand on the sensor surface. Coupling is based on the same principles developed for affinity chromatography, and a variety of sensor chips from BIACORE provide several alternatives. The gold surface of the basic sensor chip (sensor chip CM5; BIACORE) is covered with linear carboxymethylated dextran, which produces a hydrophilic environment and net negative charge that reduces nonspecific adsorption. Cysteine residues are usually less abundant in proteins than lysines, and therefore coupling through thiol groups is likely to result in a less heterogeneous or even uniform orientation of the coupled protein on the surface. Some proteins cannot be immobilized on the chip directly because they cannot be attracted to the surface (preconcentrated), or they irreversibly denature under the coupling conditions. In such cases, biotinylation is a good alternative because it can be performed in solution and under conditions preserving protein activity. Biotinylated protein is immobilized by injection across an SA chip, following an initial conditioning of the surface by three 1-minute pulses of 1 M NaC1–50 m M NaOH solution, as recommended by Biacore." @default.
• W1548749287 created "2016-06-24" @default.
• W1548749287 creator A5059459393 @default.
• W1548749287 creator A5087824725 @default.
• W1548749287 date "2000-01-01" @default.
• W1548749287 modified "2023-09-27" @default.
• W1548749287 title "[2] Analysis of protein-protein interactions in phototransduction cascade using surface plasmon resonance" @default.
• W1548749287 cites W1016500918 @default.
• W1548749287 cites W1505041459 @default.
• W1548749287 cites W1513138125 @default.
• W1548749287 cites W1670420367 @default.
• W1548749287 cites W1963792323 @default.
• W1548749287 cites W1967556830 @default.
• W1548749287 cites W1971563068 @default.
• W1548749287 cites W1980292799 @default.
• W1548749287 cites W1995152766 @default.
• W1548749287 cites W1996213171 @default.
• W1548749287 cites W1997604473 @default.
• W1548749287 cites W2008478712 @default.
• W1548749287 cites W2010669949 @default.
• W1548749287 cites W2014899116 @default.
• W1548749287 cites W2017362198 @default.
• W1548749287 cites W2017962609 @default.
• W1548749287 cites W2023016770 @default.
• W1548749287 cites W2031095951 @default.
• W1548749287 cites W2035068690 @default.
• W1548749287 cites W2042383380 @default.
• W1548749287 cites W2047663089 @default.
• W1548749287 cites W2061133720 @default.
• W1548749287 cites W2061873937 @default.
• W1548749287 cites W2064258616 @default.
• W1548749287 cites W2064775549 @default.
• W1548749287 cites W2066860834 @default.
• W1548749287 cites W2082411432 @default.
• W1548749287 cites W2084882448 @default.
• W1548749287 cites W2092173694 @default.
• W1548749287 cites W2111668132 @default.
• W1548749287 cites W2118888416 @default.
• W1548749287 cites W2145572269 @default.
• W1548749287 cites W2159646945 @default.
• W1548749287 cites W2171642228 @default.
• W1548749287 cites W2409028660 @default.
• W1548749287 cites W2413424377 @default.
• W1548749287 cites W966756668 @default.
• W1548749287 doi "https://doi.org/10.1016/s0076-6879(00)16714-x" @default.
• W1548749287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10800666" @default.
• W1548749287 hasPublicationYear "2000" @default.
• W1548749287 type Work @default.
• W1548749287 sameAs 1548749287 @default.
• W1548749287 citedByCount "9" @default.
• W1548749287 countsByYear W15487492872014 @default.
• W1548749287 crossrefType "book-chapter" @default.
• W1548749287 hasAuthorship W1548749287A5059459393 @default.
• W1548749287 hasAuthorship W1548749287A5087824725 @default.
• W1548749287 hasConcept C106847996 @default.
• W1548749287 hasConcept C12554922 @default.
• W1548749287 hasConcept C131584629 @default.
• W1548749287 hasConcept C131667965 @default.
• W1548749287 hasConcept C147789679 @default.
• W1548749287 hasConcept C150394285 @default.
• W1548749287 hasConcept C155672457 @default.
• W1548749287 hasConcept C171250308 @default.
• W1548749287 hasConcept C178790620 @default.
• W1548749287 hasConcept C185592680 @default.
• W1548749287 hasConcept C191897082 @default.
• W1548749287 hasConcept C192562407 @default.
• W1548749287 hasConcept C204628709 @default.
• W1548749287 hasConcept C2776190903 @default.
• W1548749287 hasConcept C2778204606 @default.
• W1548749287 hasConcept C2780358262 @default.
• W1548749287 hasConcept C2780827179 @default.
• W1548749287 hasConcept C43617362 @default.
• W1548749287 hasConcept C55493867 @default.
• W1548749287 hasConcept C86803240 @default.
• W1548749287 hasConcept C88001094 @default.
• W1548749287 hasConceptScore W1548749287C106847996 @default.
• W1548749287 hasConceptScore W1548749287C12554922 @default.
• W1548749287 hasConceptScore W1548749287C131584629 @default.
• W1548749287 hasConceptScore W1548749287C131667965 @default.
• W1548749287 hasConceptScore W1548749287C147789679 @default.
• W1548749287 hasConceptScore W1548749287C150394285 @default.
• W1548749287 hasConceptScore W1548749287C155672457 @default.
• W1548749287 hasConceptScore W1548749287C171250308 @default.
• W1548749287 hasConceptScore W1548749287C178790620 @default.
• W1548749287 hasConceptScore W1548749287C185592680 @default.
• W1548749287 hasConceptScore W1548749287C191897082 @default.
• W1548749287 hasConceptScore W1548749287C192562407 @default.
• W1548749287 hasConceptScore W1548749287C204628709 @default.
• W1548749287 hasConceptScore W1548749287C2776190903 @default.
• W1548749287 hasConceptScore W1548749287C2778204606 @default.
• W1548749287 hasConceptScore W1548749287C2780358262 @default.
• W1548749287 hasConceptScore W1548749287C2780827179 @default.
• W1548749287 hasConceptScore W1548749287C43617362 @default.
• W1548749287 hasConceptScore W1548749287C55493867 @default.
• W1548749287 hasConceptScore W1548749287C86803240 @default.
• W1548749287 hasConceptScore W1548749287C88001094 @default.
• W1548749287 hasLocation W15487492871 @default.
• W1548749287 hasLocation W15487492872 @default.
• W1548749287 hasOpenAccess W1548749287 @default.
• W1548749287 hasPrimaryLocation W15487492871 @default.

• next