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- W1548990523 abstract "THE BRmIVr fluorescence on the long arm of the Y chromosome in human mitoses and interphase nuclei stained with quinacrine mustard [1, 2, 3] or quinacrine [4] seems to be an excellent tool for the detection of this chromosome, even in cases when its presence may not be proved by other morphological criteria [5]. The wide range of chromosomal rearrangements and the difficulties to obtain properly spread mitoses in most solid turnouts interfere with their exact karyotyping. However, the quinacrine technique enables us to determine readily at least the fluorescent part of the male sex chromosome in human neoplasias [6]. We have investigated the occurrence of Y body (YB) in interphase nuclei of human malignant epithelial tumours from 18 males (Table 1) and 9 females. The material was obtained either by autopsy performed within 24 hr of death or surgically. In most cases the non-tumorous liver tissue or gastrointestinal mucosa were also investigated. A 0.Scm ~ volume of tissues was minced by scissors and then thoroughly squashed. The squashed tissue, diluted in physiological saline, was filtered through gauze to obtain a homogeneous cell suspension. Alter centritugation the pellet was fixed in methanol. Cell smears were stained with quinacrine hydrochloride according to" @default.
- W1548990523 created "2016-06-24" @default.
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- W1548990523 date "1972-10-01" @default.
- W1548990523 modified "2023-09-25" @default.
- W1548990523 title "Absence of fluorescent Y bodies in some malignant epithelial tumours in human males" @default.
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- W1548990523 doi "https://doi.org/10.1016/0014-2964(72)90109-0" @default.
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