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• W1549271283 startingPage "529" @default.
• W1549271283 abstract "In the mutant CC-1047 of Chlamydomonas reinhardtii, LDS-PAGE showed that the chlorophyll-protein complex I (CPI) is almost absent. The mutant could not grow in a culture medium without organic carbon source while the wild type (WT) C. reinhardtii grew quickly. When an organic carbon source was added into the culture medium, the mutant grew almost as well as WT. The rate of photosystem 1 (PS1) electron transport (DCPIP→MV) and the rate of whole chain electron transport (H2O→MV) of chloroplasts of the CC-1047 mutant were both lower than those of WT. The photophosphorylation activity, photosynthetic O2 evolution rate, and rate of NADP+ photoreduction of CC-1047 were also much lower than the activities of WT. There were some differences in ATPase activity between the mutant and WT. Two different activation ways were used to activate the latent ATPase using methanol and dithiothreitol (DTT) as activation substrate. More methanol and DTT were required for the mutant than WT to obtain the maximum activity. Thus the photosynthetic apparatus could not operate normally when CPI was absent because of the abnormal PS1 electron transport. Meanwhile, the other adjacent complexes of the thylakoid membrane, for example, ATP synthase complex, were slightly affected." @default.
• W1549271283 created "2016-06-24" @default.
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• W1549271283 date "2002-12-01" @default.
• W1549271283 modified "2023-10-16" @default.
• W1549271283 title "Effect of Chlorophyll-Protein Complex I Deficiency on the Physiological Character of a Chlamydomonas reinhardtii Mutant" @default.
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• W1549271283 doi "https://doi.org/10.1023/a:1024391700452" @default.
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