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- W1549366943 abstract "A nitrogen-phosphorus detection-gas chromatographic method, which provides improved sensitivity and selectivity for diphenhydramine, is reported. A 25 m X 0.31 mm cross-linked, 5% phenylmethyl silicone-coated fused-silica capillary column (film thickness 0.52 micron) was used for all analyses. The splitless capillary injection mode was employed with a 2-microliter sample being introduced by an automatic liquid sampler. Standard curves, using orphenadrine as an internal standard, were linear in the range 2-320 ng of diphenhydramine per 0.5 ml of sheep plasma. This represents an amount of diphenhydramine from ca. 40 pg to 6.4 ng at the detector. Chromatographic separation of diphenhydramine and orphenadrine was excellent, with no interference from endogenous plasma constituents. Applicability of the method was demonstrated by a placental transfer study in a chronically instrumented pregnant sheep following a 100 mg intravenous injection of diphenhydramine to the ewe." @default.
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- W1549366943 date "1986-01-01" @default.
- W1549366943 modified "2023-10-18" @default.
- W1549366943 title "Determination of diphenhydramine in biological fluids by capillary gas chromatography using nitrogen—phosphorus detection" @default.
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- W1549366943 doi "https://doi.org/10.1016/s0378-4347(00)80734-3" @default.
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