Matches in SemOpenAlex for { <https://semopenalex.org/work/W1549375498> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1549375498 endingPage "10383" @default.
- W1549375498 startingPage "10378" @default.
- W1549375498 abstract "It was found previously that the enzyme ubiquitin-protein ligase (E3) contains specific protein substrate binding sites that are responsible for the selection of proteins for degradation by the ubiquitin system. In the present study, we have tried to gain more insight into the mode of action of E3 by the characterization of other binding sites of this enzyme. Following the ligation of ubiquitin to 125I-lysozyme, the conjugates produced are very tightly bound to E3, as indicated by size analysis on glycerol density gradient centrifugation. The strong binding of ubiquitin-protein conjugates to the enzyme may account for the apparently processive addition of multiple molecules of ubiquitin to the protein substrate. Both the protein substrate moiety and the ubiquitin moiety participate in the interaction of ubiquitin-protein conjugates with E3, as indicated by competition with specific agents and by the comparison of the binding of ubiquitin-conjugated protein to that of free protein. In addition to the binding of its substrates and products, E3 also appears to interact with some of the enzymes with which it acts in concert. When E3 is incubated with the ubiquitin-carrier protein E2, a complex is formed between the two enzymes as analyzed on glycerol gradients. The formation of an E2.E3 complex may facilitate the transfer of activated ubiquitin from E2 to the protein substrate bound to the ligase." @default.
- W1549375498 created "2016-06-24" @default.
- W1549375498 creator A5001675710 @default.
- W1549375498 creator A5031356617 @default.
- W1549375498 creator A5035630459 @default.
- W1549375498 date "1989-06-01" @default.
- W1549375498 modified "2023-10-14" @default.
- W1549375498 title "Binding Sites of Ubiquitin-Protein Ligase" @default.
- W1549375498 cites W1479851678 @default.
- W1549375498 cites W1489931243 @default.
- W1549375498 cites W1513849811 @default.
- W1549375498 cites W1519470329 @default.
- W1549375498 cites W1529813906 @default.
- W1549375498 cites W1566774081 @default.
- W1549375498 cites W1607209090 @default.
- W1549375498 cites W1611482599 @default.
- W1549375498 cites W1901920202 @default.
- W1549375498 cites W1968229731 @default.
- W1549375498 cites W1998475203 @default.
- W1549375498 cites W2034941135 @default.
- W1549375498 cites W2071056869 @default.
- W1549375498 cites W2083370021 @default.
- W1549375498 cites W2146437721 @default.
- W1549375498 cites W2169996826 @default.
- W1549375498 doi "https://doi.org/10.1016/s0021-9258(18)81630-1" @default.
- W1549375498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2732227" @default.
- W1549375498 hasPublicationYear "1989" @default.
- W1549375498 type Work @default.
- W1549375498 sameAs 1549375498 @default.
- W1549375498 citedByCount "67" @default.
- W1549375498 countsByYear W15493754982012 @default.
- W1549375498 countsByYear W15493754982013 @default.
- W1549375498 countsByYear W15493754982014 @default.
- W1549375498 countsByYear W15493754982019 @default.
- W1549375498 countsByYear W15493754982020 @default.
- W1549375498 crossrefType "journal-article" @default.
- W1549375498 hasAuthorship W1549375498A5001675710 @default.
- W1549375498 hasAuthorship W1549375498A5031356617 @default.
- W1549375498 hasAuthorship W1549375498A5035630459 @default.
- W1549375498 hasBestOaLocation W15493754981 @default.
- W1549375498 hasConcept C104317684 @default.
- W1549375498 hasConcept C134459356 @default.
- W1549375498 hasConcept C140248907 @default.
- W1549375498 hasConcept C175114707 @default.
- W1549375498 hasConcept C185592680 @default.
- W1549375498 hasConcept C25602115 @default.
- W1549375498 hasConcept C2908868211 @default.
- W1549375498 hasConcept C51639874 @default.
- W1549375498 hasConcept C552990157 @default.
- W1549375498 hasConcept C55493867 @default.
- W1549375498 hasConcept C86803240 @default.
- W1549375498 hasConcept C95444343 @default.
- W1549375498 hasConceptScore W1549375498C104317684 @default.
- W1549375498 hasConceptScore W1549375498C134459356 @default.
- W1549375498 hasConceptScore W1549375498C140248907 @default.
- W1549375498 hasConceptScore W1549375498C175114707 @default.
- W1549375498 hasConceptScore W1549375498C185592680 @default.
- W1549375498 hasConceptScore W1549375498C25602115 @default.
- W1549375498 hasConceptScore W1549375498C2908868211 @default.
- W1549375498 hasConceptScore W1549375498C51639874 @default.
- W1549375498 hasConceptScore W1549375498C552990157 @default.
- W1549375498 hasConceptScore W1549375498C55493867 @default.
- W1549375498 hasConceptScore W1549375498C86803240 @default.
- W1549375498 hasConceptScore W1549375498C95444343 @default.
- W1549375498 hasIssue "18" @default.
- W1549375498 hasLocation W15493754981 @default.
- W1549375498 hasOpenAccess W1549375498 @default.
- W1549375498 hasPrimaryLocation W15493754981 @default.
- W1549375498 hasRelatedWork W1964924844 @default.
- W1549375498 hasRelatedWork W1966031852 @default.
- W1549375498 hasRelatedWork W2005618651 @default.
- W1549375498 hasRelatedWork W2104538866 @default.
- W1549375498 hasRelatedWork W2168753845 @default.
- W1549375498 hasRelatedWork W2348681936 @default.
- W1549375498 hasRelatedWork W2511325721 @default.
- W1549375498 hasRelatedWork W2905907959 @default.
- W1549375498 hasRelatedWork W2992624417 @default.
- W1549375498 hasRelatedWork W4295897835 @default.
- W1549375498 hasVolume "264" @default.
- W1549375498 isParatext "false" @default.
- W1549375498 isRetracted "false" @default.
- W1549375498 magId "1549375498" @default.
- W1549375498 workType "article" @default.