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- W1549532521 abstract "This chapter reviews that sensitive and accurate kinetic measurements of ionized metal ions are central for the understanding of ion transport in biological systems. Among the various cations interacting with biological materials, H+, Ca2+, and Mg2+ have a key role in regulating enzyme activity and cellular functions. Hence, the study of H+, Ca2+, and Mg2+ transport acquires particular significance for the understanding of (1) energy transduction and cellular events such as contraction, secretion, fusion, and excitation and (2) more complex events such as vision and hormone and neurotransmitter action. The chapter also discusses the methods for measuring H+, Ca2+, and Mg2+, which includes isotope distribution, atomic absorption spectroscopy, specific electrodes, photoluminescent, fluorescence, and absorbance indicators. All of these methods present advantages and disadvantages with respect to selectivity, specificity, time resolution, and sensitivity. It also reviews that metallochromic indicators are substances that undergo color changes when the concentration of free metal ion in the solution changes. The color difference between the free indicator and the indicator-metal ion complex is often very large and can be used for quantitative determination of the concentration of free metal ion in solution." @default.
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- W1549532521 date "1979-01-01" @default.
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- W1549532521 title "[27] Measurements of cation transport with metallochromic indicators" @default.
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- W1549532521 doi "https://doi.org/10.1016/0076-6879(79)56030-3" @default.
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