Matches in SemOpenAlex for { <https://semopenalex.org/work/W1549582007> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W1549582007 endingPage "5" @default.
- W1549582007 startingPage "3721" @default.
- W1549582007 abstract "The human colon tumor cell line HCT116 is deficient in wild-type hMLH1, is defective in mismatch repair (MMR), exhibits microsatellite instability, and is tolerant to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Transferring a normal copy of hMLH1 on chromosome 3 into the cell line restores MMR activity, stabilizes microsatellite loci, and increases the sensitivity of the cell to MNNG. Previous studies in other cell lines tolerant to alkylating agents such as MNNG or N-methylnitrosourea have shown cross-tolerance to 6-thioguanine (6TG), leading to a hypothesis that tolerance to MNNG or 6TG may be the result of MMR deficiency. To test this hypothesis, we studied the effects of 6TG on the MNNG-tolerant, MMR-deficient HCT116 cell line and its MNNG-sensitive, MMR-proficient, MNNG-tolerant, and MMR-deficient derivatives. Continuous exposure to low doses of 6TG (0.31-1.25 micrograms/ml) had no apparent effect on colony-forming ability (CFA) in MNNG-tolerant, MMR-deficient cells, whereas MNNG-sensitive, MMR-proficient cells exhibited a dose-dependent decrease in CFA. Growth kinetics and cell cycle analysis revealed that the growth of 6TG-treated HCT116 + chr3 cells was arrested at G2 after exposure to low dose of 6TG. In contrast, the same exposure to 6TG did not induce G2 arrest but rather a G1 delay in HCT116 and HCT116 + chr2. To obtain further evidence for the role of MMR on 6TG and MNNG toxicity, we isolated an MNNG-resistant revertant clone, M2, from the MNNG-sensitive, MMR-proficient HCT116 + chr3 cell line and characterized the MMR activity, hMLH1 status, and 6TG response. The results showed that M2 cells lost MMR activity as well as the previously introduced normal hMLH1 gene. Restoration of the CFA of M2 and an absence of G2 arrest were observed after treatment with low doses of 6TG. These results suggest that the mismatch repair system interacts with the G2 checkpoint in response to 6TG or MNNG-induced DNA lesions. The results further suggest that any agent that induces DNA mispairs will cause G2 arrest in MMR-proficient cells but not in MMR-deficient cells." @default.
- W1549582007 created "2016-06-24" @default.
- W1549582007 creator A5012301986 @default.
- W1549582007 creator A5023899260 @default.
- W1549582007 creator A5043978359 @default.
- W1549582007 creator A5075993653 @default.
- W1549582007 creator A5077415209 @default.
- W1549582007 creator A5078547421 @default.
- W1549582007 creator A5081334740 @default.
- W1549582007 date "1995-09-01" @default.
- W1549582007 modified "2023-10-18" @default.
- W1549582007 title "Evidence for a connection between the mismatch repair system and the G2 cell cycle checkpoint." @default.
- W1549582007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7641183" @default.
- W1549582007 hasPublicationYear "1995" @default.
- W1549582007 type Work @default.
- W1549582007 sameAs 1549582007 @default.
- W1549582007 citedByCount "135" @default.
- W1549582007 countsByYear W15495820072012 @default.
- W1549582007 countsByYear W15495820072013 @default.
- W1549582007 countsByYear W15495820072014 @default.
- W1549582007 countsByYear W15495820072016 @default.
- W1549582007 countsByYear W15495820072018 @default.
- W1549582007 countsByYear W15495820072019 @default.
- W1549582007 countsByYear W15495820072020 @default.
- W1549582007 countsByYear W15495820072023 @default.
- W1549582007 crossrefType "journal-article" @default.
- W1549582007 hasAuthorship W1549582007A5012301986 @default.
- W1549582007 hasAuthorship W1549582007A5023899260 @default.
- W1549582007 hasAuthorship W1549582007A5043978359 @default.
- W1549582007 hasAuthorship W1549582007A5075993653 @default.
- W1549582007 hasAuthorship W1549582007A5077415209 @default.
- W1549582007 hasAuthorship W1549582007A5078547421 @default.
- W1549582007 hasAuthorship W1549582007A5081334740 @default.
- W1549582007 hasConcept C104317684 @default.
- W1549582007 hasConcept C105696609 @default.
- W1549582007 hasConcept C134935766 @default.
- W1549582007 hasConcept C1491633281 @default.
- W1549582007 hasConcept C153911025 @default.
- W1549582007 hasConcept C180754005 @default.
- W1549582007 hasConcept C2779767149 @default.
- W1549582007 hasConcept C29537977 @default.
- W1549582007 hasConcept C502942594 @default.
- W1549582007 hasConcept C54355233 @default.
- W1549582007 hasConcept C552990157 @default.
- W1549582007 hasConcept C60748783 @default.
- W1549582007 hasConcept C61320498 @default.
- W1549582007 hasConcept C62112901 @default.
- W1549582007 hasConcept C81089528 @default.
- W1549582007 hasConcept C81885089 @default.
- W1549582007 hasConcept C86803240 @default.
- W1549582007 hasConceptScore W1549582007C104317684 @default.
- W1549582007 hasConceptScore W1549582007C105696609 @default.
- W1549582007 hasConceptScore W1549582007C134935766 @default.
- W1549582007 hasConceptScore W1549582007C1491633281 @default.
- W1549582007 hasConceptScore W1549582007C153911025 @default.
- W1549582007 hasConceptScore W1549582007C180754005 @default.
- W1549582007 hasConceptScore W1549582007C2779767149 @default.
- W1549582007 hasConceptScore W1549582007C29537977 @default.
- W1549582007 hasConceptScore W1549582007C502942594 @default.
- W1549582007 hasConceptScore W1549582007C54355233 @default.
- W1549582007 hasConceptScore W1549582007C552990157 @default.
- W1549582007 hasConceptScore W1549582007C60748783 @default.
- W1549582007 hasConceptScore W1549582007C61320498 @default.
- W1549582007 hasConceptScore W1549582007C62112901 @default.
- W1549582007 hasConceptScore W1549582007C81089528 @default.
- W1549582007 hasConceptScore W1549582007C81885089 @default.
- W1549582007 hasConceptScore W1549582007C86803240 @default.
- W1549582007 hasIssue "17" @default.
- W1549582007 hasLocation W15495820071 @default.
- W1549582007 hasOpenAccess W1549582007 @default.
- W1549582007 hasPrimaryLocation W15495820071 @default.
- W1549582007 hasRelatedWork W1972088169 @default.
- W1549582007 hasRelatedWork W1975715157 @default.
- W1549582007 hasRelatedWork W2131161059 @default.
- W1549582007 hasRelatedWork W2153616465 @default.
- W1549582007 hasRelatedWork W2284507934 @default.
- W1549582007 hasRelatedWork W2357329258 @default.
- W1549582007 hasRelatedWork W2357728028 @default.
- W1549582007 hasRelatedWork W2374592133 @default.
- W1549582007 hasRelatedWork W2389703265 @default.
- W1549582007 hasRelatedWork W2965708936 @default.
- W1549582007 hasVolume "55" @default.
- W1549582007 isParatext "false" @default.
- W1549582007 isRetracted "false" @default.
- W1549582007 magId "1549582007" @default.
- W1549582007 workType "article" @default.