Matches in SemOpenAlex for { <https://semopenalex.org/work/W1549666314> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1549666314 endingPage "11881" @default.
- W1549666314 startingPage "11876" @default.
- W1549666314 abstract "Calsequestrin (Mr = 44,000) is a calcium-binding (KD congruent to 1 mM, congruent to 50 sites/molecule) protein found in the lumen of the sarcoplasmic reticulum of skeletal muscle. The 1H NMR spectrum of calsequestrin in the calcium-free form is presented and is characteristic of a protein largely in the random coil configuration. A number of peaks in the aromatic region have been assigned based on their chemical shifts and sensitivity to pH. The interaction of this protein with Ca2+ and K+ was studied by 1H NMR. Potassium ion binding to calsequestrin caused broadening and concomitant loss of intensity in both the aromatic and aliphatic regions of the spectrum. Calcium ion binding caused similar effects but at much lower metal ion concentrations. It was found that the binding of Ca2+ to calsequestrin was cooperative (Hill coefficient n = 2.9 +/- 0.2) with a dissociation constant of 0.25 +/- 0.06 mM in the absence of K+. In contrast, K+ showed binding to a single class of independent sites (KD = 0.20 +/- 0.04 M). Calcium binding was also studied by circular dichroism at protein concentrations similar to the NMR experiments. The binding profile and cooperativity (n = 2.0 +/- 0.1, KD = 0.19 +/- 0.04 mM) were in agreement with the 1H NMR results. Circular dichroism studies performed at low protein concentrations to reduce the possible effect of calcium binding on the concentration of free calcium gave similar values of n = 2.42 +/- 0.14 and KD = 0.21 +/- 0.005 mM. This cooperativity was also observed in the presence of 100 mM KCl although the affinity for calcium has been significantly reduced (n = 1.65 +/- 0.09, KD = 0.87 +/- 0.036 mM). In view of the large number of calcium binding sites in calsequestrin, these small Hill coefficients show that calcium binding to calsequestrin is only mildly cooperative." @default.
- W1549666314 created "2016-06-24" @default.
- W1549666314 creator A5002229676 @default.
- W1549666314 creator A5048773279 @default.
- W1549666314 creator A5067477469 @default.
- W1549666314 creator A5087326906 @default.
- W1549666314 date "1984-10-01" @default.
- W1549666314 modified "2023-10-16" @default.
- W1549666314 title "Characterization of skeletal muscle calsequestrin by 1H NMR spectroscopy." @default.
- W1549666314 cites W1482605569 @default.
- W1549666314 cites W1500397819 @default.
- W1549666314 cites W1531532159 @default.
- W1549666314 cites W1531765690 @default.
- W1549666314 cites W1543140440 @default.
- W1549666314 cites W1571243084 @default.
- W1549666314 cites W1775749144 @default.
- W1549666314 cites W1980839154 @default.
- W1549666314 cites W1998093640 @default.
- W1549666314 cites W2005392755 @default.
- W1549666314 cites W2007709380 @default.
- W1549666314 cites W2030036949 @default.
- W1549666314 cites W2053584696 @default.
- W1549666314 cites W2062228103 @default.
- W1549666314 cites W2070696315 @default.
- W1549666314 cites W2088194826 @default.
- W1549666314 cites W2100837269 @default.
- W1549666314 cites W2172587676 @default.
- W1549666314 cites W7592053 @default.
- W1549666314 doi "https://doi.org/10.1016/s0021-9258(20)71293-7" @default.
- W1549666314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6480588" @default.
- W1549666314 hasPublicationYear "1984" @default.
- W1549666314 type Work @default.
- W1549666314 sameAs 1549666314 @default.
- W1549666314 citedByCount "49" @default.
- W1549666314 countsByYear W15496663142012 @default.
- W1549666314 countsByYear W15496663142013 @default.
- W1549666314 countsByYear W15496663142015 @default.
- W1549666314 countsByYear W15496663142020 @default.
- W1549666314 countsByYear W15496663142022 @default.
- W1549666314 crossrefType "journal-article" @default.
- W1549666314 hasAuthorship W1549666314A5002229676 @default.
- W1549666314 hasAuthorship W1549666314A5048773279 @default.
- W1549666314 hasAuthorship W1549666314A5067477469 @default.
- W1549666314 hasAuthorship W1549666314A5087326906 @default.
- W1549666314 hasBestOaLocation W15496663141 @default.
- W1549666314 hasConcept C105702510 @default.
- W1549666314 hasConcept C113217602 @default.
- W1549666314 hasConcept C121332964 @default.
- W1549666314 hasConcept C12554922 @default.
- W1549666314 hasConcept C158617107 @default.
- W1549666314 hasConcept C171250308 @default.
- W1549666314 hasConcept C185592680 @default.
- W1549666314 hasConcept C192562407 @default.
- W1549666314 hasConcept C2775953344 @default.
- W1549666314 hasConcept C2779959927 @default.
- W1549666314 hasConcept C2780841128 @default.
- W1549666314 hasConcept C46141821 @default.
- W1549666314 hasConcept C55493867 @default.
- W1549666314 hasConcept C66974803 @default.
- W1549666314 hasConcept C71240020 @default.
- W1549666314 hasConcept C86803240 @default.
- W1549666314 hasConceptScore W1549666314C105702510 @default.
- W1549666314 hasConceptScore W1549666314C113217602 @default.
- W1549666314 hasConceptScore W1549666314C121332964 @default.
- W1549666314 hasConceptScore W1549666314C12554922 @default.
- W1549666314 hasConceptScore W1549666314C158617107 @default.
- W1549666314 hasConceptScore W1549666314C171250308 @default.
- W1549666314 hasConceptScore W1549666314C185592680 @default.
- W1549666314 hasConceptScore W1549666314C192562407 @default.
- W1549666314 hasConceptScore W1549666314C2775953344 @default.
- W1549666314 hasConceptScore W1549666314C2779959927 @default.
- W1549666314 hasConceptScore W1549666314C2780841128 @default.
- W1549666314 hasConceptScore W1549666314C46141821 @default.
- W1549666314 hasConceptScore W1549666314C55493867 @default.
- W1549666314 hasConceptScore W1549666314C66974803 @default.
- W1549666314 hasConceptScore W1549666314C71240020 @default.
- W1549666314 hasConceptScore W1549666314C86803240 @default.
- W1549666314 hasIssue "19" @default.
- W1549666314 hasLocation W15496663141 @default.
- W1549666314 hasOpenAccess W1549666314 @default.
- W1549666314 hasPrimaryLocation W15496663141 @default.
- W1549666314 hasRelatedWork W1981661164 @default.
- W1549666314 hasRelatedWork W1983739817 @default.
- W1549666314 hasRelatedWork W1992915597 @default.
- W1549666314 hasRelatedWork W2026628029 @default.
- W1549666314 hasRelatedWork W2040730438 @default.
- W1549666314 hasRelatedWork W2043210674 @default.
- W1549666314 hasRelatedWork W2087375815 @default.
- W1549666314 hasRelatedWork W2093820398 @default.
- W1549666314 hasRelatedWork W3037456415 @default.
- W1549666314 hasRelatedWork W3110907406 @default.
- W1549666314 hasVolume "259" @default.
- W1549666314 isParatext "false" @default.
- W1549666314 isRetracted "false" @default.
- W1549666314 magId "1549666314" @default.
- W1549666314 workType "article" @default.