FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1549782011> ?p ?o ?g. }

• W1549782011 endingPage "225" @default.
• W1549782011 startingPage "217" @default.
• W1549782011 abstract "Limulus amebocyte lysate was fractionated by heparin-Sepharose chromatography into four components (fractions A, B, C and D). Major coagulation factors, i.e., proclotting enzyme, coagulogen, and proclotting enzyme activating factor precursor (proactivator) in the lysate were eluted, respectively, in fraction A, fraction B and fraction C. Clotting enzyme activity was detected only following recombination of fraction A and fraction C in the presence of endotoxin. The conversion of proactivator to its active form (activator) was an endotoxin-dependent reaction and was inhibited by polymyxin B. Either proactivator is an endotoxin-sensitive factor or another endotoxin-sensitive factor, which activates proactivator, is present in fraction C. Optimal pH for proclotting enzyme activation by activator was broad and ranged from pH 6.0 to 8.0, while that for the endotoxin-mediated activation of proactivator was pH 7.0. No initial latent period was observed during activation of the proactivator or proenzyme. The activator was inhibited by benzamidine, leupeptin, soybean trypsin inhibitor and diisopropyl fluorophosphate, suggesting that the activator is a trypsin-type serine protease. Trypsin, but not thrombin, urokinase, plasmin, papain or alpha-chymotrypsin activated the proclotting enzyme. Therefore, limited proteolysis, i.e., of an arginyl- or lysyl-X bond(s), of the proenzyme molecule is probably involved in its activation." @default.
• W1549782011 created "2016-06-24" @default.
• W1549782011 creator A5070425276 @default.
• W1549782011 creator A5078297770 @default.
• W1549782011 date "1982-10-01" @default.
• W1549782011 modified "2023-09-27" @default.
• W1549782011 title "Fractionation of Limulus amebocyte lysate" @default.
• W1549782011 cites W1484115349 @default.
• W1549782011 cites W14937535 @default.
• W1549782011 cites W1558024408 @default.
• W1549782011 cites W1562797470 @default.
• W1549782011 cites W1625660792 @default.
• W1549782011 cites W171312770 @default.
• W1549782011 cites W1859811746 @default.
• W1549782011 cites W1974730906 @default.
• W1549782011 cites W1975176230 @default.
• W1549782011 cites W1976775709 @default.
• W1549782011 cites W1980235078 @default.
• W1549782011 cites W1988295650 @default.
• W1549782011 cites W2001193614 @default.
• W1549782011 cites W2007630390 @default.
• W1549782011 cites W2018641297 @default.
• W1549782011 cites W2023733345 @default.
• W1549782011 cites W2051272166 @default.
• W1549782011 cites W2055252145 @default.
• W1549782011 cites W2069676891 @default.
• W1549782011 cites W2085717873 @default.
• W1549782011 cites W2102829518 @default.
• W1549782011 cites W2103374224 @default.
• W1549782011 cites W2139236541 @default.
• W1549782011 cites W2154756612 @default.
• W1549782011 cites W2174628005 @default.
• W1549782011 cites W2271316673 @default.
• W1549782011 cites W2411433873 @default.
• W1549782011 cites W2412671666 @default.
• W1549782011 cites W2623326407 @default.
• W1549782011 doi "https://doi.org/10.1016/0167-4838(82)90354-5" @default.
• W1549782011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7138884" @default.
• W1549782011 hasPublicationYear "1982" @default.
• W1549782011 type Work @default.
• W1549782011 sameAs 1549782011 @default.
• W1549782011 citedByCount "22" @default.
• W1549782011 countsByYear W15497820112019 @default.
• W1549782011 countsByYear W15497820112022 @default.
• W1549782011 crossrefType "journal-article" @default.
• W1549782011 hasAuthorship W1549782011A5070425276 @default.
• W1549782011 hasAuthorship W1549782011A5078297770 @default.
• W1549782011 hasConcept C104317684 @default.
• W1549782011 hasConcept C181199279 @default.
• W1549782011 hasConcept C185592680 @default.
• W1549782011 hasConcept C2775883766 @default.
• W1549782011 hasConcept C2776714187 @default.
• W1549782011 hasConcept C2776873056 @default.
• W1549782011 hasConcept C2779288520 @default.
• W1549782011 hasConcept C2779832904 @default.
• W1549782011 hasConcept C2780340462 @default.
• W1549782011 hasConcept C2781071845 @default.
• W1549782011 hasConcept C2781378782 @default.
• W1549782011 hasConcept C43617362 @default.
• W1549782011 hasConcept C52853521 @default.
• W1549782011 hasConcept C55493867 @default.
• W1549782011 hasConcept C57409179 @default.
• W1549782011 hasConcept C88045685 @default.
• W1549782011 hasConceptScore W1549782011C104317684 @default.
• W1549782011 hasConceptScore W1549782011C181199279 @default.
• W1549782011 hasConceptScore W1549782011C185592680 @default.
• W1549782011 hasConceptScore W1549782011C2775883766 @default.
• W1549782011 hasConceptScore W1549782011C2776714187 @default.
• W1549782011 hasConceptScore W1549782011C2776873056 @default.
• W1549782011 hasConceptScore W1549782011C2779288520 @default.
• W1549782011 hasConceptScore W1549782011C2779832904 @default.
• W1549782011 hasConceptScore W1549782011C2780340462 @default.
• W1549782011 hasConceptScore W1549782011C2781071845 @default.
• W1549782011 hasConceptScore W1549782011C2781378782 @default.
• W1549782011 hasConceptScore W1549782011C43617362 @default.
• W1549782011 hasConceptScore W1549782011C52853521 @default.
• W1549782011 hasConceptScore W1549782011C55493867 @default.
• W1549782011 hasConceptScore W1549782011C57409179 @default.
• W1549782011 hasConceptScore W1549782011C88045685 @default.
• W1549782011 hasFunder F4320320912 @default.
• W1549782011 hasFunder F4320337338 @default.
• W1549782011 hasIssue "2" @default.
• W1549782011 hasLocation W15497820111 @default.
• W1549782011 hasLocation W15497820112 @default.
• W1549782011 hasOpenAccess W1549782011 @default.
• W1549782011 hasPrimaryLocation W15497820111 @default.
• W1549782011 hasRelatedWork W1549782011 @default.
• W1549782011 hasRelatedWork W1568336365 @default.
• W1549782011 hasRelatedWork W2034411994 @default.
• W1549782011 hasRelatedWork W2035681261 @default.
• W1549782011 hasRelatedWork W2051999408 @default.
• W1549782011 hasRelatedWork W2058221331 @default.
• W1549782011 hasRelatedWork W2068118913 @default.
• W1549782011 hasRelatedWork W2079957910 @default.
• W1549782011 hasRelatedWork W2083123461 @default.
• W1549782011 hasRelatedWork W2192299483 @default.
• W1549782011 hasVolume "707" @default.
• W1549782011 isParatext "false" @default.

• next