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- W1549887344 abstract "Abstract The amino acid sequence of glycolate oxidase from spinach has been fitted to an electron density map at 2.2 A resolution. From a refined model we give a detailed description of the flavin mononucleotide-binding site and the residues which might be involved in the catalytic action of the enzyme. The cofactor is bound to the enzyme at the carboxy end of the beta strands in the alpha/beta barrel domain and forms a number of hydrogen bonds both to residues at the end of the beta strands and in the loop regions after the strands. In particular Lys-230 interacts with atoms N1 and O2 of the isoalloxazine ring, which produces an inductive effect that could enhance the nucleophilicity of the electron acceptor N5 of the flavin ring. Almost the entire coenzyme is buried in the interior of the enzyme. The exceptions are one phosphate oxygen atom and a region around the N5 position on the si-face of the isoalloxazine ring, which are accessible to solution. Based on a model of bound glycolate to our structure we propose that the following residues are important in the catalytic reaction: Arg-257, Tyr-24, and Tyr-129 for binding the substrate, and His-254 for abstracting a proton from the C2 atom of the substrate." @default.
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- W1549887344 date "1989-02-01" @default.
- W1549887344 modified "2023-10-02" @default.
- W1549887344 title "The Active Site of Spinach Glycolate Oxidase" @default.
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- W1549887344 doi "https://doi.org/10.1016/s0021-9258(18)94112-8" @default.
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