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• W1550041976 abstract "PKN is a serine/threonine protein kinase, which has a catalytic domain highly homologous to that of protein kinase C (PKC) in the carboxyl-terminal region and three repeats of the antiparallel coiled coil (ACC) domain in the amino-terminal region. Mammalian PKN has three isoforms each derived from different genes, PKN1 (PKNalpha/PRK1/PAK1), PKN2 (PRK2/PAK2/PKNgamma), and PKN3 (PKNbeta). PKN isoforms show different enzymatic properties and tissue distributions and have been implicated in various distinct cellular processes (reviewed in Mukai [2003]). This chapter discusses methods to prepare purified enzymes and to assay substrate phosphorylation activities." @default.
• W1550041976 created "2016-06-24" @default.
• W1550041976 creator A5019968973 @default.
• W1550041976 creator A5079051183 @default.
• W1550041976 date "2006-01-01" @default.
• W1550041976 modified "2023-10-09" @default.
• W1550041976 title "Purification and Kinase Assay of PKN" @default.
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• W1550041976 doi "https://doi.org/10.1016/s0076-6879(06)06017-4" @default.
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