FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1550101598> ?p ?o ?g. }

• W1550101598 endingPage "295" @default.
• W1550101598 startingPage "288" @default.
• W1550101598 abstract "Background Hydrodynamic injection of pmCMVenh-hEF-1prom-muIFN-γ, a plasmid DNA (pDNA) expressing murine interferon (IFN)-γ with a murine cytomegalovirus (mCMV) enhancer and a human elongation factor (EF)-1 promoter, has been proven effective for the treatment of cancer and atopic dermatitis in mice. However, the initial peak of IFN-γ soon after injection was quite high compared to the steady level for subsequent periods, which could cause unwanted adverse effects. Therefore, in the present study, aiming to optimize the efficacy/side-effect ratio of IFN-γ gene transfer, we have developed plasmid vectors encoding murine IFN-γ under the control of different combinations of promoter and enhancer sequences. Methods The promoter and enhancer sequence of pmCMVenh-hEF-1prom-huIFN-γ, a prototype plasmid expressing human IFN-γ, was replaced or deleted to obtain various pDNAs. To assess the transgene expression profile, each pDNA was delivered to mice by hydrodynamic injection and the serum IFN-γ concentration was measured periodically. On the basis of the results obtained, murine IFN-γ expressing pDNAs were constructed and the body weight change was monitored as an indicator of adverse effects. Results The prototype pmCMVenh-hEF-1prom-huIFN-γ showed a high but declining concentration of IFN-γ. Those containing hROSA26 promoter expressed the transgene in a more constant manner with no initial high concentrations and scarcely reduced the body weight. Conclusions These results indicate that hROSA26 promoter, irrespective of the presence and type of enhancers, is suitable for achieving constant and steady level of transgene expression and effective in avoiding the body weight loss caused by high concentrations of IFN-γ. Copyright © 2012 John Wiley & Sons, Ltd." @default.
• W1550101598 created "2016-06-24" @default.
• W1550101598 creator A5020909884 @default.
• W1550101598 creator A5028043688 @default.
• W1550101598 creator A5035791716 @default.
• W1550101598 creator A5050031455 @default.
• W1550101598 creator A5071041099 @default.
• W1550101598 date "2012-04-01" @default.
• W1550101598 modified "2023-09-27" @default.
• W1550101598 title "Constant and steady transgene expression of interferon-γ by optimization of plasmid construct for safe and effective interferon-γ gene therapy" @default.
• W1550101598 cites W1860174057 @default.
• W1550101598 cites W1937560614 @default.
• W1550101598 cites W1967686432 @default.
• W1550101598 cites W1974700453 @default.
• W1550101598 cites W1979002716 @default.
• W1550101598 cites W1986655312 @default.
• W1550101598 cites W1995080846 @default.
• W1550101598 cites W1999358069 @default.
• W1550101598 cites W2006846934 @default.
• W1550101598 cites W2009482935 @default.
• W1550101598 cites W2014691694 @default.
• W1550101598 cites W2022515405 @default.
• W1550101598 cites W2033901933 @default.
• W1550101598 cites W2037587513 @default.
• W1550101598 cites W2039618053 @default.
• W1550101598 cites W2045733748 @default.
• W1550101598 cites W2060564760 @default.
• W1550101598 cites W2075068603 @default.
• W1550101598 cites W2092096067 @default.
• W1550101598 cites W2094208778 @default.
• W1550101598 cites W2126770689 @default.
• W1550101598 cites W2149556394 @default.
• W1550101598 cites W2151165319 @default.
• W1550101598 cites W2320147764 @default.
• W1550101598 cites W4248227985 @default.
• W1550101598 doi "https://doi.org/10.1002/jgm.2616" @default.
• W1550101598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22359360" @default.
• W1550101598 hasPublicationYear "2012" @default.
• W1550101598 type Work @default.
• W1550101598 sameAs 1550101598 @default.
• W1550101598 citedByCount "13" @default.
• W1550101598 countsByYear W15501015982012 @default.
• W1550101598 countsByYear W15501015982013 @default.
• W1550101598 countsByYear W15501015982014 @default.
• W1550101598 countsByYear W15501015982015 @default.
• W1550101598 countsByYear W15501015982017 @default.
• W1550101598 countsByYear W15501015982018 @default.
• W1550101598 countsByYear W15501015982020 @default.
• W1550101598 countsByYear W15501015982021 @default.
• W1550101598 crossrefType "journal-article" @default.
• W1550101598 hasAuthorship W1550101598A5020909884 @default.
• W1550101598 hasAuthorship W1550101598A5028043688 @default.
• W1550101598 hasAuthorship W1550101598A5035791716 @default.
• W1550101598 hasAuthorship W1550101598A5050031455 @default.
• W1550101598 hasAuthorship W1550101598A5071041099 @default.
• W1550101598 hasConcept C102230213 @default.
• W1550101598 hasConcept C104317684 @default.
• W1550101598 hasConcept C111599444 @default.
• W1550101598 hasConcept C111936080 @default.
• W1550101598 hasConcept C150194340 @default.
• W1550101598 hasConcept C153911025 @default.
• W1550101598 hasConcept C159047783 @default.
• W1550101598 hasConcept C22744801 @default.
• W1550101598 hasConcept C2776178377 @default.
• W1550101598 hasConcept C40767141 @default.
• W1550101598 hasConcept C54009773 @default.
• W1550101598 hasConcept C54355233 @default.
• W1550101598 hasConcept C86803240 @default.
• W1550101598 hasConceptScore W1550101598C102230213 @default.
• W1550101598 hasConceptScore W1550101598C104317684 @default.
• W1550101598 hasConceptScore W1550101598C111599444 @default.
• W1550101598 hasConceptScore W1550101598C111936080 @default.
• W1550101598 hasConceptScore W1550101598C150194340 @default.
• W1550101598 hasConceptScore W1550101598C153911025 @default.
• W1550101598 hasConceptScore W1550101598C159047783 @default.
• W1550101598 hasConceptScore W1550101598C22744801 @default.
• W1550101598 hasConceptScore W1550101598C2776178377 @default.
• W1550101598 hasConceptScore W1550101598C40767141 @default.
• W1550101598 hasConceptScore W1550101598C54009773 @default.
• W1550101598 hasConceptScore W1550101598C54355233 @default.
• W1550101598 hasConceptScore W1550101598C86803240 @default.
• W1550101598 hasIssue "4" @default.
• W1550101598 hasLocation W15501015981 @default.
• W1550101598 hasLocation W15501015982 @default.
• W1550101598 hasOpenAccess W1550101598 @default.
• W1550101598 hasPrimaryLocation W15501015981 @default.
• W1550101598 hasRelatedWork W2359175313 @default.
• W1550101598 hasRelatedWork W2363386379 @default.
• W1550101598 hasRelatedWork W2365306427 @default.
• W1550101598 hasRelatedWork W2367323167 @default.
• W1550101598 hasRelatedWork W2375453033 @default.
• W1550101598 hasRelatedWork W2376869834 @default.
• W1550101598 hasRelatedWork W2383434177 @default.
• W1550101598 hasRelatedWork W2389479801 @default.
• W1550101598 hasRelatedWork W2394425474 @default.
• W1550101598 hasRelatedWork W2915002842 @default.
• W1550101598 hasVolume "14" @default.
• W1550101598 isParatext "false" @default.

• next